Physicochemical characterization and antimicrobial activity against Erwinia amylovora, Erwinia vitivora, and Diplodia seriata of a light purple Hibiscus syriacus L. Cultivar

Phytochemicals are essential raw materials for the production of formulations that can be helpful in crop protection. In particular, Hibiscus spp., which are often used in traditional medicine, are rich in potential bioactive molecules. This study presents an analysis of the thermal, vibrational, and phytochemical characteristics of a light purple variety of Hibiscus syriacus, using thermal gravimetric and differential scanning calorimetry, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectroscopy techniques. Further, with a view to its valorization, the antimicrobial activity of its extracts has been investigated in vitro against Erwinia amylovora (the phytopathogen responsible for fire blight in apples, pears, and some other members of the family Rosaceae), Erwinia vitivora (the causal agent of the “maladie d’Oléron” in grapevines), and Diplodia seriata (responsible for “Bot canker”). Higher heating values and thermal features showed similarities with kenaf biomass. The main compounds identified in the hydro-methanolic extracts were: in flowers, 1-heptacosanol, heptacosane, 1-tetracosanol, hexadecenoic acid, 9,12,15-octadecatrienoic acid, and 9,12-octadecadienoic acid; and in leaves, the coumarin derivative 4,4,6,8-tetramethyl-2-chromanone, vitamin E, phytol, and sitosterol. MIC values of 500 and 375 μg·mL−1 were obtained against E. amylovora for flower and leaf extracts, respectively, upon conjugation with chitosan oligomers (to improve solubility and bioavailability). In the case of E. vitivora, MIC values of 250 and 500 μg·mL−1, respectively, were registered. Regarding the antifungal activity, EC90 values of 975.8 and 603.5 μg·mL−1, respectively, were found. These findings suggest that H. syriacus (cv. ‘Mathilde’) may be a promising source of antimicrobials for agriculture

Sex-Specific Effects of High Yolk Androgen Levels on Constitutive and Cell-Mediated Immune Responses in Nestlings of an Altricial Passerine

Avian embryos are exposed to yolk androgens that are incorporated into the egg by the ovulating female. These steroids can affect several aspects of embryo development, often resulting in increases in overall size or the speed of growth of different traits. However, several studies suggest that they also entail immune costs to the offspring. In this study, we explored whether variation in yolk androgen concentration affected several measures of the constitutive and cell-mediated immune axes in the spotless starling (Sturnus unicolor). Using a within-brood design, we injected different doses of androgens (testosterone and androstenedione) into the eggs. Our study showed that experimentally increased yolk androgens led to sex-specific immunosuppression in both the innate and adaptive axes of the immune system. Both cell-mediated immune response (CMI) and lysozyme activity decreased with increasing androgen levels injected into the egg in the case of male nestlings, whereas there were no effects on females. The effects that we found were always linear: no quadratic or threshold patterns were detected. We found no effects of the experimental treatment in hemolysis or agglutination capacity, but these measures were negatively correlated with CMI, suggesting negative correlation among different branches of the immune system. Blood (trypanosomes and hemosporidians) and intestinal (coccidia) parasites were not affected by the experimental increase of yolk androgen levels. Our results show that in our study species yolk androgens induce immunosuppression in some axes of the male nestling immune system. Further studies should analyze the proximate causes for these contrasting effects in different axes of the immune system and the reason for the differential impact on males and females

Ionized and neutral gas in the peculiar star/cluster complex in NGC 6946

The characteristics of ionized and HI gas in the peculiar star/cluster complex in NGC 6946, obtained with the 6-m telescope (BTA) SAO RAS, the Gemini North telescope, and the Westerbork Synthesis Radio Telescope (WSRT), are presented. The complex is unusual as hosting a super star cluster, the most massive known in an apparently non-interacting giant galaxy. It contains a number of smaller clusters and is bordered by a sharp C-shaped rim. We found that the complex is additionally unusual in having peculiar gas kinematics. The velocity field of the ionized gas reveals a deep oval minimum, ~300 pc in size, centered 7" east of the supercluster. The Vr of the ionized gas in the dip center is 100 km/s lower than in its surroundings, and emission lines within the dip appear to be shock excited. This dip is near the center of an HI hole and a semi-ring of HII regions. The HI (and less certainly, HII) velocity fields reveal expansion, with the velocity reaching ~30 km/s at a distance about 300 pc from the center of expansion, which is near the deep minimum position. The super star cluster is at the western rim of the minimum. The sharp western rim of the whole complex is plausibly a manifestation of a regular dust arc along the complex edge. Different hypotheses about the complex and the Vr depression origins are discussed, including a HVC/dark mini-halo impact, a BCD galaxy merging, and a gas outflow due to release of energy from the supercluster stars.Comment: MN RAS, accepte

Open Peer Review Module (OPRM). Final Report

Research productivity is increasing at an unprecedented rate. Technological innovations, a surge in available computing power, and the ease with which digital information is stored and communicated is helping researchers to cross experimentation boundaries, to increase data availability, and to facilitate the transfer of knowledge. As a result, traditional research is being transformed into a dynamic and globally interconnected effort where ideas, tools and results can be made instantly accessible to the entire academic community. Institutional and multidisciplinary open access repositories play a crucial role in this emerging landscape by enabling immediate accessibility to all kinds of research output. One important element still missing from open access repositories, however, is a quantitative assessment of the hosted research items that will facilitate the process of selecting the most relevant and distinguished content. Common currently available metrics, such as number of visits and downloads, do not reflect the quality of a research work, which can only be assessed directly by peers offering their expert opinion together with quantitative ratings based on specific criteria. To address this issue we developed an Open Peer Review Module (OPRM) to be installed on existing open access repositories and offered as an overlay service. Any digital research work hosted in a compliant repository can then be evaluated by an unlimited number of peers who offer not only a qualitative assessment in the form of text, but also quantitative measures that are used to build the reputation of the research work and its authors. Crucially, this evaluation system is open and transparent. By open we mean that the full text of the peer reviews are publicly available along with the original research work. By transparent we mean that the identity of the reviewers is disclosed to the authors and to the public. In our model, openness and transparency are two elemental aspects we consider necessary to address the issue of biased or non-expert opinions, which is inherent in the anonymous peer review model, characterized by the unaccountability of reviewers. Importantly, our open peer review module includes a reviewer reputation system based on the assessment of reviews themselves by other peer reviewers. This allows a sophisticated scaling of the importance of each review on the overall assessment of a research work, based on the reputation of the reviewer. The implementation of a peer review layer on top of institutional repositories could have the potential to transform the current academic publication landscape by introducing new scholarly workflows where a research item can be openly evaluated by the world’s experts right at the institutional repository of its authors, before being submitted to an academic journal. This workflow challenges the current practices of peer review research evaluation. In most cases, journals, acting as brands in a competitive market, foster academic competition for a limited number of publication slots, instead of promoting open scholarship and collaboration. The integration of peer review in repositories will enable direct and transparent academic collaboration between authors and reviewers. In addition, the use of the OPRM will produce novel metrics directly reflecting the perceived quality of a research work by expert peers, contrary to current available altmetrics that only indirectly account for quality through usage statistics.OpenAIR

Matching microscopic and macroscopic responses in glasses

We first reproduce on the Janus and Janus II computers a milestone experiment that measures the spin-glass coherence length through the lowering of free-energy barriers induced by the Zeeman effect. Secondly we determine the scaling behavior that allows a quantitative analysis of a new experiment reported in the companion Letter [S. Guchhait and R. Orbach, Phys. Rev. Lett. 118, 157203 (2017)]. The value of the coherence length estimated through the analysis of microscopic correlation functions turns out to be quantitatively consistent with its measurement through macroscopic response functions. Further, non-linear susceptibilities, recently measured in glass-forming liquids, scale as powers of the same microscopic length.Comment: 6 pages, 4 figure

The Mpemba effect in spin glasses is a persistent memory effect

The Mpemba effect occurs when a hot system cools faster than an initially colder one, when both are refrigerated in the same thermal reservoir. Using the custom built supercomputer Janus II, we study the Mpemba effect in spin glasses and show that it is a non-equilibrium process, governed by the coherence length \xi of the system. The effect occurs when the bath temperature lies in the glassy phase, but it is not necessary for the thermal protocol to cross the critical temperature. In fact, the Mpemba effect follows from a strong relationship between the internal energy and \xi that turns out to be a sure-tell sign of being in the glassy phase. Thus, the Mpemba effect presents itself as an intriguing new avenue for the experimental study of the coherence length in supercooled liquids and other glass formers.Comment: Version accepted for publication in PNAS. 6 pages, 7 figure

Spectrophotometric Observations of Blue Compact Dwarf Galaxies: Mrk 370

We present results from a detailed spectrophotometric analysis of the blue compact dwarf galaxy (BCD) Mrk 370, based on deep UBVRI broad-band and Halpha narrow-band observations, and long-slit and two-dimensional spectroscopy of its brightest knots. The spectroscopic data are used to derive the internal extinction, and to compute metallicities, electronic density and temperature in the knots. By subtracting the contribution of the underlying older stellar population, modeled by an exponential function, removing the contribution from emission lines, and correcting for extinction, we can measure the true colors of the young star-forming knots. We show that the colors obtained this way differ significantly from those derived without the above corrections, and lead to different estimates of the ages and star-forming history of the knots. Using predictions of evolutionary synthesis models, we estimate the ages of both the starburst regions and the underlying stellar component. We found that we can reproduce the colors of all the knots with an instantaneous burst of star formation and the Salpeter initial mass function with an upper mass limit of 100 solar masses. The resulting ages range between 3 and 6 Myrs. The colors of the low surface brightness component are consistent with ages larger than 5 Gyr. The kinematic results suggest ordered motion around the major axis of the galaxy.Comment: 26 pages with 14 figures; accepted for publication in Ap

Nature of the spin-glass phase at experimental length scales

We present a massive equilibrium simulation of the three-dimensional Ising spin glass at low temperatures. The Janus special-purpose computer has allowed us to equilibrate, using parallel tempering, L=32 lattices down to T=0.64 Tc. We demonstrate the relevance of equilibrium finite-size simulations to understand experimental non-equilibrium spin glasses in the thermodynamical limit by establishing a time-length dictionary. We conclude that non-equilibrium experiments performed on a time scale of one hour can be matched with equilibrium results on L=110 lattices. A detailed investigation of the probability distribution functions of the spin and link overlap, as well as of their correlation functions, shows that Replica Symmetry Breaking is the appropriate theoretical framework for the physically relevant length scales. Besides, we improve over existing methodologies to ensure equilibration in parallel tempering simulations.Comment: 48 pages, 19 postscript figures, 9 tables. Version accepted for publication in the Journal of Statistical Mechanic