Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens

Atmospheric pressure plasmas have gained attention in recent years for several environmental applications. This technology could potentially be used to deactivate airborne microorganisms, surface-bound microorganisms, and biofilms. In this work, the authors explore the efficacy of the atmospheric pressure dielectric barrier discharge (DBD) to inactivate airborne Staphylococcus epidermidis and Aspergillus niger that are opportunistic pathogens associated with nosocomial infections. This technology uses air as the source of gas and does not require any process gas such as helium, argon, nitrogen, or hydrogen. The effect of DBD was studied on aerosolized S. epidermidis and aerosolized A. niger spores via scanning electron microscopy (SEM). The morphology observed on the SEM micrographs showed deformations in the cellular structure of both microor- ganisms. Cell structure damage upon interaction with the DBD suggests leakage of vital cellular materials, which is a key mechanism for microbial inactivation. The chemical structure of the cell surface of S. epidermidis was also analyzed by near edge x-ray absorption fine structure spectros- copy before and after DBD exposure. Results from surface analysis revealed that reactive oxygen species from the DBD discharge contributed to alterations on the chemistry of the cell membrane/ cell wall of S. epidermidis

Postharvest heat treatments to inhibit Penicillium digitatum growth and maintain quality of Mandarin (Citrus reticulata blanco)

Use of fungicides is a common practice as a postharvest treatment to control fruit decay. Nowadays, environment-friendly technologies, such as heat treatments, are viable replacements. This study evaluated the effects of post-harvest heat treatments (traditional and microwave-assisted) on mandarins intentionally inoculated with Penicillium digitatum. For the studied heat treatments, the target temperature was 50C, which was held for 2.5 min. After heating, mandarins were cooled and stored at 25C for 13 days. MW treatments effectively prevented mold growth during storage, while HW only delayed it. Control mandarins (without treatment) showed the highest significant weight loss. Neither thermal treatment nor storage affected fruit juice pH (p \u3e 0.05). Treated mandarins had a significantly lower vitamin C content than control fruits throughout storage, and all mandarins lost firmness by the 13th day (p \u3c 0.05). Control and MW-treated mandarins had lower citric acid content; however, they retained color, total soluble solids (TSS) and had a higher maturity index. While HW mandarins did not have changes in citric acid content, they had higher TSS, and lower maturity index. MW-assisted treatments were effective at inactivating molds and helped retain some nutritional and physical-chemical characteristics of mandarins. However, juice of MW-treated mandarins was not preferred by judges in the sensory tests, the juice was rated lower than that obtained from the other treatment. Postharvest heat treatments may constitute a helpful application to control mandarin’ fungal decay

TH3.2: Youth transitions and livestock in Kenya

Globally, youth transitions to adulthood are becoming more complex. The relative importance of key milestones that are used to describe the transition from youth to adulthood are culturally embedded and change across generations. So called ‘critical moments' may include completing education, moving from school to the labour force, leaving home, marriage, and parenthood. In the global south, sub–Saharan Africa in particular, agricultural and livestock assets are also culturally valued and often critical to supporting major achievements in transitions. What can we learn from youth studies to better understand youth engagement in agriculture and livestock sectors? This work aims to bridge youth and gender studies to explore young women's and men's transitions and the various ways that livestock feature in the achievement of key milestones. What are young women's and men's values and experiences as they enter adulthood? How does livestock feature in youth transitions and for whom? Recognizing that gender and age intersect to create and constrain youth opportunities and experiences, we undertake a gendered analysis of livestock value chain actors to explore their perspectives on the importance and attainment of milestones. We use a mixed-methods approach that includes 700 market surveys, 38 focus group discussions, and 20 key informant interviews in 6 counties in Kenya that include pastoral, agro-pastoral, and mixed crop systems in both peri-urban and rural settings. We describe the shifts in cultural values across diverse contexts over time and the various roles that livestock play in supporting youth transitions

FR2.3: Participatory Rangeland Management: Understanding Women's Engagement and Implications for Social Change

Participatory Rangeland Management (PRM) works with customary institutions in dryland pastoral settings to strengthen communities' abilities to manage their rangelands. The process includes the creation of management committees that develop plans to manage resources that support resilience to climate change, such as restoration of grazing areas. This study describes the process of women's engagement in PRM and what it means for women's participation in decision-making processes in resource management and broader gender relations in the community and household. The study takes place in 4 communities in Baringo County, Kenya, an area severely affected by climate change effects such as drought. We use a mixed-methods approach and draw upon 56 intra-household interviews, 34 key informant interviews, and 8 FGDs. We first contextualize social change in pastoral settings, that includes describing the influence of gender sensitization efforts of development organizations and increasing numbers of women assuming leadership positions. Women actively participate in PRM committees through negotiating and advocating for protection of resources, notably those that they frequently use, such as water sources. Women also earn income from PRM related activities, that garners more support from their spouses to attend meetings. Women have also taken up so called ‘men's' activities such as beekeeping. PRM processes, that are part of wider social, economic and environmental change, bolster women's participation in decision making processes across multiple domains. Constraints however persist and limit women's potential to lead groups that include men, attend seminars, and source labor to manage domestic tasks while they participate in civic activities. These findings contribute to empirical research concerning governance of rangelands, to understanding the potential of existing frameworks to measure women's participation and provide practical lessons regarding gender responsive development

National, regional, and global causes of mortality in 5-19-year-olds from 2000 to 2019 : a systematic analysis

Background: Investments in the survival of older children and adolescents (aged 5-19 years) bring triple dividends for now, their future, and the next generation. However, 1·5 million deaths occurred in this age group globally in 2019, nearly all from preventable causes. To better focus the attention of the global community on improving survival of children and adolescents and to guide effective policy and programmes, sound and timely cause of death data are crucial, but often scarce. Methods: In this systematic analysis, we provide updated time-series for 2000-19 of national, regional, and global cause of death estimates for 5-19-year-olds with age-sex disaggregation. We estimated separately for countries with high versus low mortality, by data availability, and for four age-sex groups (5-9-year-olds [both sexes], 10-14-year-olds [both sexes], 15-19-year-old females, and 15-19-year-old males). Only studies reporting at least two causes of death were included in our analysis. We obtained empirical cause of death data through systematic review, known investigator tracing, and acquisition of known national and subnational cause of death studies. We adapted the Bayesian Least Absolute Shrinkage and Selection Operator approach to address data scarcity, enhance covariate selection, produce more robust estimates, offer increased flexibility, allow country random effects, propagate coherent uncertainty, and improve model stability. We harmonised all-cause mortality estimates with the UN Inter-agency Group for Child Mortality Estimation and systematically integrated single cause estimates as needed from WHO and UNAIDS. Findings: In 2019, the global leading specific causes of death were road traffic injuries (115 843 [95% uncertainty interval 110 672-125 054] deaths; 7·8% [7·5-8·1]); neoplasms (95 401 [90 744-104 812]; 6·4% [6·1-6·8]); malaria (81 516 [72 150-94 477]; 5·5% [4·9-6·2]); drowning (77 460 [72 474-85 952]; 5·2% [4·9-5·5]); and diarrhoea (72 679 [66 599-82 002], 4·9% [4·5-5·3]). The leading causes varied substantially across regions. The contribution of communicable, maternal, perinatal, and nutritional conditions declined with age, whereas the number of deaths associated with injuries increased. The leading causes of death were diarrhoea (51 630 [47 206-56 235] deaths; 10·0% [9·5-10·5]) in 5-9-year-olds; malaria (31 587 [23 940-43 116]; 8·6% [6·6-10·4]) in 10-14-year-olds; self-harm (32 646 [29 530-36 416]; 13·4% [12·6-14·3]) in 15-19-year-old females; and road traffic injuries (48 757 [45 692-52 625]; 13·9% [13·3-14·3]) in 15-19-year-old males. Widespread declines in cause-specific mortality were estimated across age-sex groups and geographies in 2000-19, with few exceptions like collective violence. Interpretation: Child and adolescent survival needs focused attention. To translate the vision into actions, more investments in the health information infrastructure for cause of death and in the related life-saving interventions are needed

Designing for change through “reflecting and doing”: the CGIAR Community of Practice on Gender- Transformative Research Methodologies

Gender-transformative change requires a commitment from everyone involved in agricultural research for development (AR4D) including organizations at international and national level, individual researchers and practitioners, farmers, development agencies, policy-makers and consumers, to transform the existing values, practices and priorities that (re)produce and perpetuate gender biases and inequities in agrifood systems. However, the adoption of a gender transformative agenda can be challenging, especially for AR4D organizations whose primary focus is not necessarily the attainment of gender equality. This paper looks at a collective, bottom-up, transformative effort within the AR4D organization of CGIAR. It advances the emerging CGIAR Community of Practice on Gender Transformative Research Methodologies (GTRM-CoP) as a case study to explore the potential of CoPs as social learning systems that create the conditions for transformation-oriented learning. Driven by an ethos of reflecting and doing anchored in critical and feminist principles and social learning praxis, the GTRM-CoP aims to be a safe space to spur reflexivity, creativity and collaboration to support existing work on gender transformation in CGIAR while re-imagining how gender in AR4D is conceptualized, negotiated and advanced. The paper focuses on the process leading to the development of the CoP, that is, designing for change, which is crucial for sustained transformation

Autophagy in Myf5+ progenitors regulates energy and glucose homeostasis through control of brown fat and skeletal muscle development

Macroautophagy (MA) regulates cellular quality control and energy balance. For example, loss of MA in aP2-positive adipocytes converts white adipose tissue (WAT) into brown adipose tissue (BAT)-like, enhancing BAT function and thereby insulin sensitivity. However, whether MA regulates early BAT development is unknown. We report that deleting Atg7 in myogenic Myf5+ progenitors inhibits MA in Myf5-cell-derived BAT and muscle. Knock out (KO) mice have defective BAT differentiation and function. Surprisingly, their body temperature is higher due to WAT lipolysis-driven increases in fatty acid oxidation in 'Beige' cells in inguinal WAT, BAT and muscle. KO mice also present impaired muscle differentiation, reduced muscle mass and glucose intolerance. Our studies show that ATG7 in Myf5+ progenitors is required to maintain energy and glucose homeostasis through effects on BAT and muscle development. Decreased MA in myogenic progenitors with age and/or overnutrition might contribute to the metabolic defects and sarcopenia observed in these conditions