Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle

Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines are available and the development of reverse genetic technologies has raised the prospect of using Eimeria spp. as recombinant vectors to express additional immunoprotective antigens. To study the ability of Eimeria to secrete foreign antigens or display them on the surface of the sporozoite, transiently transfected populations of E. tenella expressing the fluorescent protein mCherry, linked to endogenous signal peptide (SP) and glycophosphatidylinositol-anchor (GPI) sequences, were examined. The SP from microneme protein EtMIC2 (SP2) allowed efficient trafficking of mCherry to cytoplasmic vesicles and following the C-terminal addition of a GPI-anchor (from surface antigen EtSAG1) mCherry was expressed on the sporozoite surface. In stable transgenic populations, mCherry fused to SP2 was secreted into the sporocyst cavity of the oocysts and after excystation, secretion was detected in culture supernatants but not into the parasitophorous vacuole after invasion. When the GPI was incorporated, mCherry was observed on the sporozites surface and in the supernatant of invading sporozoites. The proven secretion and surface exposure of mCherry suggests that antigen fusions with SP2 and GPI of EtSAG1 may be promising candidates to examine induction of protective immunity against heterologous pathogens

Single and Many Particle Correlation Functions and Uniform Phase Bases for Strongly Correlated Systems

The need for suitable many or infinite fermion correlation functions to describe some low dimensional strongly correlated systems is discussed. This is linked to the need for a correlated basis, in which the ground state may be postive definite, and in which single particle correlations may suffice. A particular trial basis is proposed, and applied to a certain quasi-1D model. The model is a strip of the 2D square lattice wrapped around a cylinder, and is related to the ladder geometries, but with periodic instead of open boundary conditions along the edges. Analysis involves a novel mean-field approach and exact diagonalisation. The model has a paramagnetic region and a Nagaoka ferromagnetic region. The proposed basis is well suited to the model, and single particle correlations in it have power law decay for the paramagnet, where the charge motion is qualitatively hard core bosonic. The mean field also leads to a BCS-type model with single particle long range order.Comment: 23 pages, in plain tex, 12 Postscript figures included. Accepted for publication in J.Physics : Condensed Matte

The Higgs Sector of the Minimal 3 3 1 Model Revisited

The mass spectrum and the eigenstates of the Higgs sector of the minimal 3 3 1 model are revisited in detail. There are discrepancies between our results and previous results by another author.Comment: 20 pages, latex, two figures. One note and one reference are adde

Using Muonic Hydrogen in Optical Spectroscopy Experiment to Detect Extra Dimensions

Considering that gravitational force might deviate from Newton's inverse-square law (ISL) and become much stronger in small scale, we propose a kind of optical spectroscopy experiment to detect this possible deviation and take electronic, muonic and tauonic hydrogen atoms as examples. This experiment might be used to indirectly detect the deviation of ISL down to nanometer scale and to explore the possibility of three extra dimensions in ADD's model, while current direct gravity tests cannot break through micron scale and go beyond two extra dimensions scenario.Comment: 9 pages, 2 figures. To appear in IJT

Valley Splitting Theory of SiGe/Si/SiGe Quantum Wells

We present an effective mass theory for SiGe/Si/SiGe quantum wells, with an emphasis on calculating the valley splitting. The theory introduces a valley coupling parameter, $v_v$, which encapsulates the physics of the quantum well interface. The new effective mass parameter is computed by means of a tight binding theory. The resulting formalism provides rather simple analytical results for several geometries of interest, including a finite square well, a quantum well in an electric field, and a modulation doped two-dimensional electron gas. Of particular importance is the problem of a quantum well in a magnetic field, grown on a miscut substrate. The latter may pose a numerical challenge for atomistic techniques like tight-binding, because of its two-dimensional nature. In the effective mass theory, however, the results are straightforward and analytical. We compare our effective mass results with those of the tight binding theory, obtaining excellent agreement.Comment: 13 pages, 7 figures. Version submitted to PR

Local spin fluctuations in iron-based superconductors: 77Se and 87Rb NMR measurements of Tl0.47Rb0.34Fe1.63Se2

We report nuclear magnetic resonance (NMR) studies of the intercalated iron selenide superconductor (Tl, Rb)$_{y}$Fe$_{2-x}$Se$_2$ ($T_c = 32$ K). Single-crystal measurements up to 480 K on both $^{77}$Se and $^{87}$Rb nuclei show a superconducting phase with no magnetic order. The Knight shifts $K$ and relaxation rates $1/T_1T$ increase very strongly with temperature above $T_c$, before flattening at 400 K. The quadratic $T$-dependence and perfect proportionality of both $K$ and $1/T_1T$ data demonstrate their origin in paramagnetic moments. A minimal model for this pseudogap-like response is not a missing density of states but two additive contributions from the itinerant electronic and local magnetic components, a framework unifying the $K$ and $1/T_1 T$ data in many iron-based superconductors

Effects of dynamical phases in Shor's factoring algorithm with operational delays

Ideal quantum algorithms usually assume that quantum computing is performed continuously by a sequence of unitary transformations. However, there always exist idle finite time intervals between consecutive operations in a realistic quantum computing process. During these delays, coherent "errors" will accumulate from the dynamical phases of the superposed wave functions. Here we explore the sensitivity of Shor's quantum factoring algorithm to such errors. Our results clearly show a severe sensitivity of Shor's factorization algorithm to the presence of delay times between successive unitary transformations. Specifically, in the presence of these {\it coherent "errors"}, the probability of obtaining the correct answer decreases exponentially with the number of qubits of the work register. A particularly simple phase-matching approach is proposed in this paper to {\it avoid} or suppress these {\it coherent errors} when using Shor's algorithm to factorize integers. The robustness of this phase-matching condition is evaluated analytically or numerically for the factorization of several integers: $4, 15, 21$, and 33.Comment: 8 pages with 5 figure