Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements

A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water.Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; ArgentinaFil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentin

Plasma-based techniques applied to the determination of 17 elements in partitioned top soils

An investigation was carried out to characterize top soils collected in a prototypical urban area impacted by industrial and agricultural activities. Soils of the studied zone receive also the contribution of traffic from the urban area, marine aerosols from the Atlantic Ocean and dust from the Patagonian steppe. Top soil samples were sampled and then sieved in two fractions (F1 < 37 μm, and 37 < F2 < 50 μm) for subsequent digestion using an acid mixture containing HCl, HNO3 and HF (6:2:1). Elemental determination of Al, As, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sb, Ti, V and Zn was carried out by inductively coupled plasma optical emission spectrometry (ICP OES) and/or inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy tests for the entire analytical procedure were performed by means of the certified reference material NIST SRM 2711 Montana soil. Limit of detection reached varied from 0.08 μg g− 1 (Cd) to 2.4 μg g− 1 (Ba). Elemental concentrations ranged from 0.10 ± 0.01 μg g− 1 (Mo) to 14.6 ± 0.6% (Ca). Aluminum, Ca, Fe, Mg and Ti (geological elements) were found at concentrations in the percentage order. The ratios of elemental concentrations Mo/Ca, Ni/Ca and Ti/Ca were identified as chemical markers suitable for distinguishing the different soil samples by exposing the relative differences in the 4-element chemical profile. The concentrations of metals and metalloids in size-fractionated top soils reported in this study will be an important tool for future studies aimed to characterize soil resuspension and its contribution to airborne particulate matter.Fil: Morales del Mastro, Anabella Mabel. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Jiménez Rebagliati, Raúl Alberto Jorge. Comisión Nacional de Energía Atómica; ArgentinaFil: Pereyra, Marcelo Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Dawidowski, Laura Elena. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Gómez, Darío. Comisión Nacional de Energía Atómica; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

On-line solid phase extraction of bismuth by FI-HG-AAS using l-proline immobilised on carbon nanotubes combined with factorial design

This study explores further the analytical capabilities of functionalised multiwalled carbon nanotubes with l-proline (pro-MWCNTs) for the reliable preconcentration and determination of Bi(III) in waters using an on-line flow injection hydride generation atomic absorption spectrometry (FI-HG-AAS) system. To avoid carbon nanoparticles aggregation, the preconcentration was carried out on a microcolumn filled with functionalised pro-MWCNTs and a low molecular weight polyethylene. The optimisation was performed by employing a full factorial design. The factors related to the optimisation process were the concentration of eluent and pH of the sample. The best performance was achieved packing the substrate in a microcolumn of 2.3mm (i.d)×25mm (pro-MWCNTs net length: 10mm), the pH was 7.0 and the concentration of the eluent (HCl) was 10% (v/v). Under optimal conditions, the adsorption capacity of the substrate was found to be 0.1mgg-1 reaching a preconcentration factor (PF) of 160. A detection limit (3σ) of 0.7ngL-1 was achieved, when using only 3.0mg of substrate. Interferences affecting Bi(III) signal were carefully studied. Precision, expressed as relative standard deviation (RSD) turned to be 1.5% at the 0.1μgL-1 level (n=10). Accuracy test for the entire analytical procedure was performed by means of the Standard Reference Material (SRM) NIST 1643e (trace elements in water) and results were in good agreement with the certified values. The system was evaluated for quantitative determination of Bi(III) in different categories of waters.Fil: Parodi, Maria Belen. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Londonio, Juan Agustín. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Polla, Griselda Ines. Comisión Nacional de Energía Atómica; ArgentinaFil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentin

On-line flow injection solid phase extraction using oxidised carbon nanotubes as the substrate for cold vapour-atomic absorption determination of Hg(II) in different kinds of water

A study was carried out to investigate the concentrations of Hg(II) in different categories of water samples at ng L1 levels. The capabilities of oxidised carbon nanotubes (ox-CNTs) were studied to assess if this substrate serves as an efficient material for Hg(II) preconcentration using an on-line flow injection cold vapour-atomic absorption spectrometry (FI-CV-AAS) system. Carbon nanotubes are characterized by a marked tendency to aggregate, which negatively affects the retention of Hg whenever integrated in flow systems as a packed column. For this reason, the preconcentration was carried out in a microcolumn filled with a mixture of ox-CNTs and a low molecular weight polyethylene. The preparation of the microcolumn was studied in detail. Concerning column design, the best performance was achieved when packing the substrate in a microcolumn of 2.25 mm (i.d.) 20 mm length. The effect of chemical and physical parameters including the pH of the solutions, the eluent type and the concentration was systematically examined. Mercury was retained at pH 5.0 and 15% (v/v) HCl was the best alternative for Hg(II) elution. Under optimal conditions, the adsorption capacity of the substrate was found to be 3.2 mg g1 reaching a preconcentration factor (PF) of 150. The high adsorption capacity of this substrate allowed reaching a detection limit (3s) of 1.9 ng L1, when using a sorbent column containing only 1.0 mg of ox-CNTs. The limit of quantification (10s) resulted to be 6.3 ng L1. Precision, expressed as relative standard deviation (RSD), turned out to be 1.6% at the 0.1 mg L1 level (n ¼ 8). The system was evaluated for quantitative determination of Hg in river water, sea water and effluents.Fil: Parodi, Maria Belen. Instituto Nacional de Tecnología Industrial; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Londonio, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Comisión Nacional de Energía Atómica. Gerencia Química. CAC; ArgentinaFil: Polla, Griselda Ines. Comisión Nacional de Energía Atómica; ArgentinaFil: Savio, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaFil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia Química. CAC; Argentin

Inductively coupled plasma optical emission spectrometric determination of fifteen elements in dietary supplements: Are the concentrations declared in the labels accurate?

A study was carried out to establish a reliable procedure for determining 15 elements (As, Bi, Cd, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sb, Se, V and Zn) in different brands of dietary supplements purchased in Argentina and USA. Supplements were digested with HNO3 and H2O2 using an optimized microwave procedure. Inductively coupled plasma optical emission spectrometry (ICP OES) was selected for total element determination. The overall approach was tested in tablets of: (i) Se supplement, (ii) two multimineral supplements, (iii) cholesterol control tablets, (iv) multivitamins for men, and (v) a multivitamin+multimineral supplement. Arsenic, Cd and Pb concentrations were in all the analyzed samples below the detection limits for these elements (As, 1.2 µg g-1; Cd, 0.09 µg g-1 and Pb, 1.5 µg g-1). Elemental concentrations of the other elements investigated showed a great variability depending on the trade mark analyzed. Measured metal concentration ranged from 0.78±0.19 µg g-1 (Ni) to 13.5±0.7 % (Ca). Most abundant elements, detected as percentage were Ca, Mg and Fe. In general terms, the study evidenced that metal content reported by the manufacturer in labels of dietary supplements agree with found values. On the other hand, significant differences in metal concentration were found among tablets of the same bottle.Fil: Marrero, Julieta. Comision Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Gerencia Ciclo del Combustible Nuclear; ArgentinaFil: Jiménez Rebagliati, Raúl Alberto Jorge. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); ArgentinaFil: Leiva, Emanuel. Comision Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Gerencia Ciclo del Combustible Nuclear; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Effects of common cooking heat treatments on selenium content and speciation in garlic

The effect of traditional thermal treatments, normally applied during cooking practices, on the stability of Se-amino acids and Se-proteins present in Se-enriched garlic was studied. Five heat treatments including, convection oven (180 °C, 10 min), boiling (100 °C, 10 min), microwave oven (800 W, 3 min), steaming (10 min) and stir-frying (180 °C, 3 min) were assayed. Modifications on the molecular weight fractions profiles of protein extracts were evaluated by size exclusion chromatography with UV–visible and inductively coupled plasma mass spectrometry (ICP-MS) detection using a 0.05 M Tris(hidroxymethyl)aminomethane solution at pH 7.3 as the mobile phase. Possible modifications of Se-amino acids were evaluated by anion exchange chromatography (AEC), with a 30 mM Na2HPO4 solution (pH 6) as the mobile phase, coupled to ICP-MS after enzymatic hydrolysis. SEC chromatograms showed the incorporation of Se to high (7 kDa) and low molecular weight fractions (2–4 kDa) while AEC chromatograms showed mostly the presence of Se-methylselenocysteine. However, it could be observed that heat treatments significantly affect this important Se-amino acid.Fil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ávila Maniero, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wuilloud, Rodolfo German. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentin

REE profiling in basic volcanic rocks after ultrasonic sample treatment and ICPMS analysis with oxide ion formation in ICP enriched with O2

ICP-MS methods, and its variants, had been extensively used to determine REEs due to their lower detection limits, high sensitivity and dynamic linear range. However, spectral interferences caused by oxide and hydroxide ions always represents an issue regardless the type of sample been dealt. In the present work it was described the study of REEs oxides formation with the introduction of an auxiliary line directly to the cyclonic chamber to generate a mix Ar/O2 plasma. Plasma conditions were optimized by studying and selecting the best flow rates to induce the formation of REEs oxides. A new analytical method for REE determination was thus proposed using ICPMS with Ar-O2 mixed plasma to aid the formation of MO ions. This method was validated through CRM analysis and through the analysis of real-world samples. The REE concentrations evaluated and normalized to chondrite REE levels allowed the analysis of basaltic rocks study in terms of their origin.Fil: Verni, Ernesto Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; ArgentinaFil: Bazan, Cristian Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Strasser, Edgardo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaFil: Perino, Ernesto Jesús. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentin

A comparative study of two nanosubstrates for the on-line solid phase extraction of antimony by FI-HG-AAS

Nanoporous carbon (NPC) prepared frommetal organic frameworks (MOFs)was studied to serve as a sorbent forpreconcentrating Sb(III) at trace levels. The performance of NPC regarding sensitivity, enrichment factor, analyticalthroughput and recovery was compared with that of multiwalled carbon nanotubes functionalised with Lproline(pro-MWCNTs). In this study, Sb(III)was preconcentrated using both substrates using an on-line flow injectionhydride generation atomic absorption spectrometry (FI-HG-AAS) system. A low molecular weight polyethylenewas added to the sorbent material to fill the preconcentration columns to avoid nanoparticleaggregation. A comprehensive study of the type and concentration of the reactionmedia and the eluentwas carriedout to select the best substrate not only in terms of adsorption capacity but also considering potentialinterferent control. The study evidenced that for Sb(III) retention, phtalate buffers (1.0 mM at pH=6) resultedthe best alternative. Under optimal conditions, both substrates exhibited high adsorption capacities:343 mg Sb g−1 NPC and 559 mg Sb g−1 pro-MWCNTs. The preconcentration factors reached were: 258 and420 for NPC and pro-MWCNTs, respectively. The high adsorption capacity of both substrate allowed reaching detectionlimits (3σ) of 1.47 and 0.52 ng L−1 for NPC and pro-MWCNTs, respectivelywhen using a sorbent columncontaining only 1.0 mg of sorbent. Precision, expressed as relative standard deviation (RSD) turned to be ~2% atthe 0.1 μg L−1 level (n=10) for both substrates. Trueness test for the entire analytical procedurewas performedby means of the Standard Reference Material (SRM) NIST 1643e (Trace elements in water) and results were ingood agreement with the certified values. The system was evaluated for quantitative determination of Sb(III)in different kind of waters. The method is simple and relatively cheap in comparison with other methodologies.Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Parodi, Belén. Universidad Nacional de San Martín; Argentina. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaFil: Alshehri, Saad M.. King Saud University; Arabia SauditaFil: Yamauchi, Yusuke. King Saud University; Arabia Saudita. Tsukuba University. National Institute For Materials Science; JapónFil: Smichowski, Patricia Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentin

On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling

An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average.EEA Concepción del UruguayFil: Farías, Silvia Sara. Comisión Nacional de Energía Atómica; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Soro, Enrique Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Chemical profile of size-fractionated soils collected in a semiarid industrial area of Argentina

A study was undertaken to assess the chemical profile of soil collected in Bahía Blanca (Argentina). In this industrial city, semiarid soils are affected by different industrial and agricultural activities, the presence of a saltpeter extraction facility, traffic and increasing urbanization. Sixteen soil samples (superficial and sub-superficial) were collected. Samples were sieved in two fractions (A Na+ ≅ SO42− > K+ > NO3−. Three indicators, namely, (i) coefficient of variation, (ii) coefficient of divergence and (iii) ratio of elemental concentration with respect to Ca were used to assess chemical, spatial and inter-profile variability. Chloride > Ca > Na+ > Mo > SO42−, dominated the variability indicating that these are key chemical markers for future assessment of crustal contribution to airborne particles in the area. The ratios Xi/Ca allowed discriminating the soil of the semi-arid region surrounding Bahía Blanca. The chemical profiles obtained in this study, particularly those of topsoil, will be a key input to characterize soil resuspension and its contribution to airborne particulate matter in a forthcoming receptor model analysis.Fil: Morales del Mastro, Anabella Mabel. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereyra, Marcelo Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereyra, Victoria. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jiménez Rebagliati, Raúl Alberto Jorge. Comisión Nacional de Energía Atómica; ArgentinaFil: Dawidowski, Laura Elena. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Dario. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin