Supplementary material for the article: Nikolic, V.; Loncarevic, B.; Popovic, A. Biodegradation of Copolymer Obtained by Grafting Reaction between Methacrylic Acid and Starch. Polymer Bulletin 2019, 76 (5), 2197–2213. https://doi.org/10.1007/s00289-018-2484-x

Supplementary material for: [https://doi.org/10.1007/s00289-018-2484-x]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/341

Supplementary material for the article: Nikolic, V.; Loncarevic, B.; Popovic, A. Biodegradation of Copolymer Obtained by Grafting Reaction between Methacrylic Acid and Starch. Polymer Bulletin 2019, 76 (5), 2197–2213. https://doi.org/10.1007/s00289-018-2484-x

Supplementary material for: [https://doi.org/10.1007/s00289-018-2484-x]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/341

Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002

Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S014765131930630X?via%3Dihub]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3158

The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth

The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3321

Oxidative transformation of levan produced by Bacillus licheniformis strain

In this work microbial polysacharide levan produced by Bacillus licheniformis NS032 was functionalized by introducing aldehyde groups into glycan chains. Resulting polyaldechide polymer was characterized by FT-IR spectral analysis and the content of aldehyde groups was determined titrimetrically

Crude extracts of cinnamon and oregano spices as components of pullulan based edible films

Pullulan based films were prepared with crude extracts of cinnamon and oregano spices. Obtained films were characterized by FTIR-ATR spectroscopy and their antioxidant and antimicrobial properties were evaluated. These biopolymer based films have potential use in food applications as food ingredients and active packaging material.6th Workshop Specific Methods for Food Safety and Quality, 27.09.2018. Belgrade, Serbi

Using Micro-Oxymax respirometer for assesment of copper toxicity on model organism Daphnia magna

Vodeni organizmi su izloženi brojnim hemijskim supstancama različitih mehanizama toksičnosti i da bi se okarakterisali međusobni efekti važna je uporedna analiza hemijskih podataka i odgovarajućeg biološkog odgovora (biomarkeri) [1]. Slatkovodni, planktonski beskičmenjak Daphnia magna je dobro poznat model u ekotoksikologiji, zbog svoje visoke osetljivosti na toksične supstance i relativno lake kultivacije u laboratorijskim uslovima. Većina istraživanja o proceni toksičnosti različitih hemijskih supstanci bazira se na ispitivanju akutne toksičosti i letalne ili efektivne koncentracije supstance koje dovode do neželjenog efekta u 50 % slučajeva (LC50/EC50), međutim neophodne su i detaljnije informacije o toksičnosti, koje bi uključile subletalne efekte i senzitivnije biomerkere [2]. Pošto bakar i njegova jedinjenja spadaju u vodeće zagađujuće supstance vode, sprovedene su i evaluirane brojne procene njegovih opasnosti i/ili negativnih efekata (US EPA 2007;EU 2007) [3]. Homeostaza bakra u akvatičnim organizmima uključuje regulisan unos,transport i ekskreciju koja je slična kao kod sisara. Studije na D. magna, koje su bile izložene subletalnim koncentracijama bakra, pokazuju pojačan oksidativni stres u jedinkama [4]. U ovoj studiji praćena je potrošnja O2 i produkcija CO2 kod jedinki D. magna tokom48 h izlaganja Cu2+ jonima. Respiracija D. magna (MicroBioTests Inc.) neonata praćena je 48 h pomoću dvanaestokanalnog Micro-Oxymax respirometra (Columbus Instruments, SAD). U intervalima od 4 h merena je koncentracija O2, paramagnetnim senzorom, a CO2 infracrvenim senzorom, pri čemu je tokom celokupnog trajanja eksprerimenta sistem bio zatvoren. Uzorci su pripremani u providnim Micro-Oxymax bocama zapremine 500 mL u kojima je bilo po 100 mL tečne podloge i 20 neonata. Korišćen je AdaM medijum (Aachener Daphnien Medium) [5]. Koncentracija Cu2+ jona bila je 50 μg/mL i odabrana je prema literaturno određenoj EC50 vrednosti za CuSO4 [6]. Jedinke nisu hranjene tokom eksperimenta i izlagane su na temperaturi od 20±2 ºC i fotoperiodu 16 h dan/8 h noć. Dobijeni podaci su evaluirani Micro-Oxymax softverom i određena je kumulativna potrošnja/proizvodnja gasova (μL). Nakon izlaganja praćena je imobilizacija neonata, a rezultati su izraženi kao procenat mortaliteta. Dva dana nakon izlaganja D. magna sa Cu2+ jonima, zabeležen je mortalitet jedinki od 75 %. Test je bio validan, jer nije zabeležen mortalitet u kontrolnoj grupi. Kod organizama koji su bili izloženi jonima bakra kumulativna potrošnja O2 je znatno manja tokom celog eksperimenta, dok je kumulativna produkcija CO2 u prvih 24 h manja, a dalje razlike nisu uočene, što se može pripisati dekompoziciji imobilisanih jedinki. Na osnovu dobijenih rezultata zaključeno je da se test na MicroOxymax respirometru može koristiti kao dodatni parametar za procenu akutnih efekata toksičnih susptanci na organizam D. magna

Combined effect of vankomycin and emodin on Staphylococcus aureus MSSA and MRSA isolates

Poster presented at: FEMS Online Conference on Microbiology 2020, Belgrade, 28-31 October 2020Abstract: [https://cer.ihtm.bg.ac.rs/handle/123456789/5149

Combined effect of vankomycin and emodin on Staphylococcus aureus MSSA and MRSA isolates

The aim of the study is to investigate the effect of vancomycin and emodin in combination on biofilm formation of two Staphylococcus aureus clinical isolates derived from wound and blood culture. Furthermore, the effect on bacterial respiration was monitored.Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5150

Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019

Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0141813018332847?via%3Dihub]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/350]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2799