Ha/tcp Scaffolds Coated By Pla And Gelatin: Preliminary In Vitro Evaluation

Tissue engineering is an interdisciplinary field that applies biological and engineering knowledge to develop replacement tissue, for example, bone tissue. Normally, scaffolds are used to provide a support for cell migration, attachment, and proliferation. However, biomimetic surface modification of scaffolds has proved to be an improvement of interaction between materials and biological tissues. These changes can induce a specific cellular response and thereby enhance the desired effects. This paper describes fabrication and coating processes of scaffolds. The coating process consist in immersed the scaffold in solutions of PLA and gelatin, followed evaporative drying in ambient temperature. For characterization were used: scanning electron microscopy (SEM), optical microscopy (OP), stereoscopy, transmission electron microscopy (TEM), X-ray microtomography (Micro-CT), X-ray diffraction (XRD) and X-ray fluorescence (XRF). The bioceramic materials used in this work showed particles size between 10 and 45 nm, and low chemical contamination. The scaffolds showed morphology with adequate porosity for tissue engineering. We suggest that the particle size used improved the process of polymer foam replication, and the coated scaffolds showed evidence of not being cytotoxic.631289294Santos, A.R., Jr., Lombello, C.B., Genari, S.C., (2012) Book Tissue Regeneration-From Basic Biology to Clinical Application, p. 512. , Chapter 16, 339-366, Edited by Prof. Jamie DaviesVogt, C.D., Xing, Q., Frost, M., Zhao, F., (2012) Biomedical Engineering Society-Advancing Human Health and Well Being, , Atlanta, Georgia, EUA(2008) Sigma Aldrich., 3 (8), pp. 1-28Li, D., Frey, M.W., Baeumner, A.J., (2006) Journal of Membrane Science, 279, pp. 354-367Mellado, P., McLlwee, H.A., Badrossamay, M.R., Goss, J.A., Mahadevan, L., Parker, K.K., (2011) Applied Physics Letters., 99, pp. 2031071-2031073Haimi, S., Suuriniemi, N., Haaparanta, A.M., Ella, V., Lindroos, B., Huhtala, H., Raty, S., Suuronen, R., (2009) Tissue Engineering Part A., 15 (7), pp. 1473-1480Dimai, H.P., Linkhart, T.A., Linkhart, S.G., Donahue, L.R., Beamer, W.G., Rosen, C.J., Farley, J.R., Baylink, D.J., (1998) Bone., 22 (3), pp. 211-216Mohamadnia, A.R., Shahbazkia, H.R., Sharifi, S., Shafaei, I., (2007) Comparative Clinical Pathology., 16 (4), pp. 265-270Rodrigues, L.R., D'Avila, M.A., Monteiro, F.J., Zavaglia, C.A.C., (2012) Materials Research., 15 (6), pp. 974-980Wu, S.C., Hsu, H.C., Hsu, S.K., Wang, W.H., Ho, W.F., (2011) Materials Characterization., 62, pp. 526-534Zhou, Z., Buchanan, F., Mitchell, C., Dunne, N., (2014) Materials Science and Engineering C., 38, pp. 1-10Franca, R., Samani, T.D., Bayade, G., Yahia, L.H., Sacher, E., (2014) Journal of Colloid and Interface Science, 420, pp. 182-188Zhu, X., Eibl, O., Berthold, C., Scheideler, L., Geis-Gerstorfer, J., (2006) Nanotechnology, 17, pp. 2711-2721Naldoni, A., Minguzzi, A., Vertova, A., Dal Santo, V., Borgesec, L., Bianchi, C.L., (2010) Journal of Materials Chemistry, 21, pp. 400-407Rodrigues, L.R., Motisuke, M., Zavaglia, C.A.C., (2009) Key Engineering Materials, 396-398, pp. 623-626Rodrigues, L.R., Motisuke, M., Zavaglia, C.A.C., Synthesis of b-TCP nanoparticles by sol-gel process (2010) 6th Latin American Congress of Artificial Organs and Biomaterials, , Gramado, Rio Grande do Sul, BrazilKalita, S.J., Bhatt, H.A., (2007) Materials Science and Engineering C., 27, pp. 837-848Motisuke, M., Carrodeguas, R.G., Zavaglia, C.A.C., (2011) Materials Research, 14 (4), pp. 493-498ASTMF1635-1

Sulforaphane loaded hyaluronic acid poloxamer hybrid hydrogel enhances cartilage protection in osteoarthritis models

Sulforaphane SFN is an isothiocyanate with anti arthritic and immuno regulatory activities, supported by the downregulation of NF amp; 954;B pathway, reduction on metalloproteinases expression and prevention of cytokine induced cartilage degeneration implicated in OA progression. SFN promising pharmacological effects associated to its possible use, by intra articular route and directly in contact to the site of action, highlight SFN as promising candidate for the development of drug delivery systems. The association of poloxamers PL and hyaluronic acid HA supports the development of osteotrophic and chondroprotective pharmaceutical formulations. This study aims to develop PL HA hybrid hydrogels as delivery systems for SFN intra articular release and evaluate their biocompatibility and efficacy for osteoarthritis treatment. All formulations showed viscoelastic behavior and cubic phase organization. SFN incorporation and drug loading showed a concentration dependent behavior following HA addition. Drug release profiles were influenced by both diffusion and relaxation of polymeric chains mechanisms. The PL407 PL338 HA SFN hydrogel did not evoke pronounced cytotoxic effects on either osteoblast or chondrosarcoma cell lines. In vitro ex vivo pharmacological evaluation interfered with an elevated activation of NF amp; 954;B and COX 2, increased the type II collagen expression, and inhibited proteoglycan depletion. These results highlight the biocompatibility and the pharmacological efficacy of PL HA hybrid hydrogels as delivery systems for SFN intra articular release for OA treatmen