Simultaneous imaging of ultrasonic relative backscatter and attenuation coefficients for quantitative liver steatosis assessment

Abstract Prevalence of liver disease is continuously increasing and nonalcoholic fatty liver disease (NAFLD) is the most common etiology. We present an approach to detect the progression of liver steatosis based on quantitative ultrasound (QUS) imaging. This study was performed on a group of 55 rats that were subjected to a control or methionine and choline deficient (MCD) diet known to induce NAFLD. Ultrasound (US) measurements were performed at 2 and 6 weeks. Thereafter, animals were humanely euthanized and livers excised for histological analysis. Relative backscatter and attenuation coefficients were simultaneously estimated from the US data and envelope signal-to-noise ratio was calculated to train a regression model for: (1) fat fraction percentage estimation and (2) performing classification according to Brunt’s criteria in grades (0 33–66%; 3, >66%) of liver steatosis. The trained regression model achieved an $$R^2$$ R 2 of 0.97 (p-value < 0.01) and a RMSE of 3.64. Moreover, the classification task reached an accuracy of 94.55%. Our results suggest that in vivo QUS is a promising noninvasive imaging modality for the early assessment of NAFLD

Understanding the Contrast Mechanism in Rotation Elastogram: A Parametric Study

Ultrasound elastography has been found to be useful in different clinical applications. For example, in breast imaging, axial strain elastography provides information related to tissue stiffness, which is used to characterize breast lesions as either benign or malignant. In addition, these lesions also differ in their bonding properties. Benign breast lesions are loosely bonded and malignant breast lesions are firmly bonded to the surrounding tissues. Therefore, only benign breast lesions will rotate/slip on the application of deformation. This rotation of lesions can be visualized with rotation elastography, which utilizes axial and lateral shear strain components. The contrast obtained in rotation elastography depends on various mechanical as well as ultrasound elastography parameters. However, there is no reported work that provides an understanding of the influence of these parameters on the visualized rotation contrast. In this work, the authors studied the rotation contrast by varying the mechanical parameters such as the inclusion b/a ratio, relative inclusion-background Young's modulus, amount of applied deformation and orientation of the inclusion. First, the authors performed finite-element analysis to understand the fundamental rotation contrast of the inclusion. Next, rotation elastograms obtained from ultrasound simulations in Field II and experiments on tissue-mimicking phantoms were investigated. Mean contrast was used as a metric to evaluate the quality of rotation elastograms in finite-element analysis, and contrast-to-noise ratio was used in Field II simulations and phantom experiments. The results indicate that rotation contrast was observed only in the case of loosely bonded inclusions. Further, the rotation contrast was found to depend on the inclusion asymmetry and its orientation with respect to the axis of deformation. Interestingly, it was found that a loosely bonded inclusion contrasts with surrounding tissue in rotation elastography, even in the absence of any inclusion-background modulus contrast

Mechanical and tribological characterization of self-lubricating Mg-SiC-Gr hybrid metal matrix composite (MMC) fabricated via mechanical alloying

This study deals with the fabrication and tribo-mechanical characterization of magnesium (Mg)-silicon carbide (SiC)- graphite (Gr) hybrid metal matrix composites (MMCs). The hybrid MMCs were fabricated by a mechanical alloying process and their mechanical and tribological properties were investigated as well as compared with those of the base material and the Mg-Gr composite. The morphology analysis of the constituent powders and the hybrid powder mixture was conducted through SEM, EDX and XRD. The microstructure of the sintered MMCs showed a uniform distribution of the reinforcement on the matrix. The developed hybrid MMCs exhibited greater mechanical properties when compared to the base material and the Mg-Gr composite. The tribological characterization of the fabricated hybrid MMCs was studied using the pin-on-disc tribometer under dry sliding conditions. The hybrid MMC composed of 5% Gr and 10%SiC (Mgþ5%Grþ10%SiC) demonstrates 92% low wear rate and 53% low friction coefficient than the matrix Mg. Therefore, this hybrid MMC can be a potential candidate to develop components used in the tribological operation

Intercellular Arc Signaling Regulates Vasodilation.

Injury responses require communication between different cell types in the skin. Sensory neurons contribute to inflammation and can secrete signaling molecules that affect non-neuronal cells. Despite the pervasive role of translational regulation in nociception, the contribution of activity-dependent protein synthesis to inflammation is not well understood. To address this problem, we examined the landscape of nascent translation in murine dorsal root ganglion (DRG) neurons treated with inflammatory mediators using ribosome profiling. We identified the activity-dependent gene, Arc, as a target of translation in vitro and in vivo Inflammatory cues promote local translation of Arc in the skin. Arc-deficient male mice display exaggerated paw temperatures and vasodilation in response to an inflammatory challenge. Since Arc has recently been shown to be released from neurons in extracellular vesicles (EVs), we hypothesized that intercellular Arc signaling regulates the inflammatory response in skin. We found that the excessive thermal responses and vasodilation observed in Arc defective mice are rescued by injection of Arc-containing EVs into the skin. Our findings suggest that activity-dependent production of Arc in afferent fibers regulates neurogenic inflammation potentially through intercellular signaling.SIGNIFICANCE STATEMENT Nociceptors play prominent roles in pain and inflammation. We examined rapid changes in the landscape of nascent translation in cultured dorsal root ganglia (DRGs) treated with a combination of inflammatory mediators using ribosome profiling. We identified several hundred transcripts subject to rapid preferential translation. Among them is the immediate early gene (IEG) Arc. We provide evidence that Arc is translated in afferent fibers in the skin. Arc-deficient mice display several signs of exaggerated inflammation which is normalized on injection of Arc containing extracellular vesicles (EVs). Our work suggests that noxious cues can trigger Arc production by nociceptors which in turn constrains neurogenic inflammation in the skin