Detection Statistics of Pulse Signals at Declinations from +42o+42^o to +52o+52^o at the Frequency 111 MHz

A search for pulse signals was carried out in a new sky area included in the monitoring program for the search for pulsars and transients. Processing of several months data recorded in six frequency channels with a total bandwidth of 2.5 MHz showed that, on average, 4 pulses per hour are observed in each of the 24 connected stationary beams. Of these pulses, 18.3% are similar to those of pulsars. They are visible in one or two neighboring beams and have a pronounced dispersion shift, that is, they are recorded first at a high and then at a low frequency, which indicates the possible passage of the signal through the interstellar medium. Almost 68% of such detected pulses belong to six known pulsars with dispersion measures from 9 to 141 $pc/cm^3$, and almost all of the remaining pulses are either noise of an unknown nature or artifacts of the proposed pulse separation technique. An additional study of the selected array of 3650 obvious pulsar pulses revealed 13 pulses belonging to four rotating radio transients (RRATs). Their dispersion measures are in the range of 17-51 $pc/cm^3$. A search for regular (periodic) RRAT emission was carried out using power spectra summed over 121 days. Periodic radiation was not detected, but for two RRATs, upper estimates of the periods were obtained from measurements of the time intervals between pulses. The upper estimates of the integrated flux density of the detected RRATs are in the range 2-4 mJy at the frequency 111 MHz.Comment: published in Astronomy Report, translated by Yandex translator with correction of scientific lexis, 6 pages, 3 figures, 2 table

Detection of two new RRATs at 111 MHz

A search for pulse signals in a area with declinations of +52\degr <\delta <+55\degr was carried out on the LPA LPI radio telescope. When processing ten months of observations recorded in six frequency channels with a channel width of 415 kHz and a total bandwidth of 2.5 MHz, 22 thousand events were found with a pronounced dispersion delay of signals over frequency channels, i.e. having signs of pulsar pulses. It turned out that the found pulses belong to four known pulsars and two new rotating radio transients (RRATs). An additional pulse search conducted in 32-channel data with a channel width of 78 kHz revealed 8 pulses for the transient J0249+52 and 7 pulses for the transient J0744+55. Periodic radiation of transients was not detected. The analysis of observations shows that the found RRATs are most likely pulsars with nullings, where the proportion of nulling is greater than 99.9\%.Comment: published in Astronomy Reports, translated by Yandex translator with correction of scientific lexis, 5 pages, 2 figures, 1 tabl

Polymorphism of genes associated with infectious lung diseases in Northern Asian populations and in patients with community-acquired pneumonia

The innate Iнн\mmune system is the first to respond to invading pathogens. It is responsible for invader recognition, immune-cell recruitment, adaptive-immunity activation, and regulation of inflammation intensity. Previously, two single-nucleotide polymorphisms of innate-immunity genes – rs5743708 (Arg753Gln) of the TLR2 gene and rs8177374 (Ser180Leu) of the TIRAP gene – have been shown to be associated with both pneumonia and tuberculosis in humans, but the data are contradictory among different ethnic groups. It has also been reported that rs10902158 at the PKP3-SIGGIR-TMEM16J genetic locus belongs to a haplotype race-specifically associated with tuberculosis. Meanwhile, a gradient of its frequency is observed in Asia. The aim of this work was to assess the effect of selection for the genotypes of the above-mentioned SNPs on the gene pools of populations living in harsh climatic conditions that contribute to the development of infectious lung diseases. We estimated the prevalence of these variants in white and Asian (Chukchis and Yakuts) population samples from Northern Asia and among patients with community-acquired pneumonia (CAP). Carriage of the rs5743708 A allele was found to predispose to severe CAP (odds ratio 2.77, p = 0.021), whereas the GG/CT genotype of rs5743708/rs8177374 proved to be protective against it (odds ratio 0.478, p = 0.022) in white patients. No association of rs10902158 with CAP (total or severe) was found among whites. Stratification of CAP by causative pathogen may help eliminate the current discrepancies between different studies. No significant difference in rs5743708 or rs8177374 was found between adolescent and long-lived white samples. Carriage of the alleles studied is probably not associated with predisposition to longevity among whites in Siberia. Both white and Asian populations studied were different from Western European and East Asian populations in the variants’ prevalence. The frequency of the rs8177374 T (Ser180Leu) variant was significantly higher in the Chukchi sample (p = 0, χ2 = 63.22) relative to the East Asian populations. This result may confirm the hypothesis about the selection of this allele in the course of human migration into areas with unfavorable climatic conditions

Individual athletes’ biomechanical features of interaction with objects in art gymnastics

Purpose: To design a biomechanical model of interaction of athlete with the subject, as well as the development of areas of its use in practice. Material: The study involved 10 students - athletes. Results: The presented computational schemes create direction of flight of different items (rope, hoop, ball, ribbon, clubs). The characteristics of the time of flight trajectories and with regard for the resistance force of the air environment. Shows the influence of initial parameters on departure flight time items. Graphic characteristics are presented trajectories of objects depending on the parameters of their departure. Conclusions: It is recommended to improve the judicial assessment and effective implementation gymnast exercises during the flight characteristics of the various items to consider trajectories of objects. Note that age, height and distance from the athletes at the end of the flight object defined biomechanical characteristics that can realize an athlete: absolute initial velocity of departure, departure angle, height of the center of mass manufacture items

Detection of Five New RRATs at 111 MHz

Results of 111-MHz monitoring observations carried out on the Big Scanning Antenna of the Pushchino Radio Astronomy Observatory during September 1-28, 2015 are presented. Fifty-four pulsating sources were detected at declinations $-9^o < \delta < +42^o$. Forty-seven of these are known pulsars, five are new sources, and two are previously discovered transients. Estimates of the peak flux densities and dispersion measures are presented or all these sources.Comment: published in Astronomy Report, translated by Yandex translator with correction of scientific lexis, 8 pages, 2 figures, 3 table

GEMINGA: NEW OBSERVATIONS AT LOW RADIO FREQUENCIES

ABSTRACT. After nearly 10 years, we have succeeded to detect radio emission from Geminga more again. In this report we present new evidence for presence of radio emission from Geminga in the range 42-112 MHz. The observations were carried out on two sensitive transit radio telescopes We used three new digital receivers to detect the pulses and to obtain dynamic spectra.The examples of mean pulse profiles are presented. Exact value of the dispersion measure have been calculated using the simultaneous observations at three frequencies

STUDYING DEVELOPMENT OF POST-VACCINAL CELLULAR IMMUNITY AGAINST BRUCELLOSIS BY MEANS OF LYMPHOCYTE <i>IN VITRO</i> TESTS USING AN EXPERIMENTAL ANTIGENIC COMPLEX

Regulatory framework and methodological approaches to evaluation of immunological effects of vaccination against brucellosis are not established, and the degree of immunological post-vaccinal rearrangement is not yet developed. Due to leading role of cellular immunity in formation of immune protection against brucellosis, evaluation the cellular response in response to antigenic stimulation may be considered the most informative and objective approach to analysis of immune changes in the body during vaccination. In order to develop the most diagnostically informative methods for design of antigen-stimulation cell tests in vitro, a careful selection of a stimulating agent (antigen) is required, which should have a sufficient activating potential, thus providing specificity of reaction under in vitro conditions. The aim of the present study is to study the in vitro specific activity of a protein-polysaccharide antigenic complex from the Brucella abortus 19 BA strain (BrAg), and an opportunity of its application in order to assess the formation of post-vaccinal cellular immunity against brucellosis.The study was performed with white laboratory mice (n = 50) immunized with the Brucella abortus 19 BA strain. The control group (n = 50) consisted of laboratory mice that received a sterile saline solution in a volume of 0.5 ml. Blood samples were taken from immunized and control animals before vaccination, and 7, 14, 21, and 30 days after immunization. By means of flow cytometry, the activation molecules CD25, CD69, MHC II and CD95, expressed on T lymphocytes (CD3+CD69+, CD3+CD25+, CD3+CD95+, CD3+MHC+) were determined. To observe the development of immunity, the intensity of expression of T lymphocyte activation markers was calculated using the stimulation quotient. BrAg was used for specific in vitro stimulation of T lymphocytes. The liquid brucellosis allergen (brucellin) was used as an antigen for comparison, when studying opportunity of BrAg usage for assessing the postvaccinal immunity development.The following results were obtained: BrAg has pronounced specific activity, it did not cause non-specific in vitro reactions (activation) of T lymphocytes, thus enabling its application as a test antigen when evaluating development of adaptive vaccine immunity against brucella.Experimental testing of brucellosis antigen for carrying out the in vitro antigen-stimulated cellular reactions, aiming for evaluation of post-vaccinal immunity development against brucellosis, showed that the usage of BrAg promotes increase in diagnostic sensitivity of cellular reactions under in vitro experimental conditions. The applied experimental antigen is a quite promising tool for development of laboratory algorithms for brucellosis diagnostics, and assessment of actual vaccination efficiency in cohorts previously vaccinated against brucellosis

Анализ эффективности цифровой реализации измерения параметров сигналов

The article reviews an algorithm of digital signal processing to implement computation of the amplitude of signal envelope, its frequency and initial phase. It also estimates performance accuracy reached owing to ideal model algorithm and its implementation. The practical implementation of the algorithm is part of the system exploring signal parameters.The goal was to test the algorithm capability to work in conditions of a priori uncertainty, having only input samples and measuring radio signal Gate [1], its capability to calculate the parameters of the radio signal with different modulation types while maintaining accuracy. Characterisation of radio signals is a very important process in radio engineering as having radio signal parameters can provide information about modulation radio signal and, consequently, information contained therein.Due to the high complexity of the algorithm, its mathematical description seems to be impossible, so construction of simulation models was chosen as a research tool. Practical implementation of the algorithm comprises a Hilbert transformer based on the FIR filter, a calculating unit of the amplitude envelope, a calculating unit of the phase detector signal, and a detector of the phase jumps. The article has discussed the algorithm in-detail and has shown deviations of values obtained in the practical implementation of the algorithm against the modeling ones. For the development and debugging of the algorithm programme, was created the Mathcad environment. The simulation results are also presented in the article.The running algorithm was tested under pulsed signal at the input, pulse signal at the FMmodulated input, and FM modulation noisy signal. Its accuracy is evaluated in comparison with the model. Dependence of envelope amplitude RMS versus dimensionality of the input signal was investigated as well. As a result, the optimum dimension of input signal was selected, as well as the accuracy of the algorithm in terms of a priori uncertainties was estimated.В статье рассмотрен алгоритм цифровой обработки сигналов, реализующий вычисление амплитуды огибающей сигнала, его частоты и начальной фазы, а так же была проведена оценка точности вычисленных характеристик. Практическая реализация алгоритма является частью системы, исследующей параметры сигнала. Целью работы было проверить способность алгоритма работать в условиях априорной неопределенности, имея на входе только отсчеты радиосигнала и измерительного строба [1]. Ввиду высокой сложности алгоритма, описание его математически не представляется возможным, поэтому в качестве аппарата для исследования было выбрано построение имитационной модели. В результате работы была исследована зависимость СКО амплитуды огибающей в зависимости от размерности входного сигнала, а так же оценена точность работы алгоритма в условиях априорной неопределенности. DOI: 10.7463/rdopt.0515.081296

Using CAST-test to investigate human specific hypersensitivity to the anthrax pathogen

We present the results of applying functional cytometric test of antigen-stimulated activation basophils to assess specific immunological reactivity in the people with anthrax, and immunized with anthrax vaccine. As a criterion for antigen-specific basophil activation, we measured expression of the CD63 membrane receptor, which reflects the process of anaphylactic basophil degranulation. To determine spontaneous and antigen-induced activation of basophils (CCR3+CD63+), a FlowCAST reagent kit (Buhlmann laboratories AG, Switzerland) was used. Anthraxin, an experimental anthrax allergen (a hydrolysate the Bacillus anthracis STI-1 strain), manufactured by the Stavropol Anti-Plague Institute, was used as a specific antigen. As based on clinical and experimental data, a threshold value of &gt; 10% of anthraxin-activated (CCR3+CD63+) basophils was accepted for the in vitro immunodiagnostic CAST test, as a laboratory criterion for the subjects exhibiting specific immune response, i.e., IgE-mediated sensitization. It was shown that, in anthrax patients within one week after onset of the disease (3-7 days), a positive CAST result was obtained in 92.3% cases; the levels of specific basophil activation with anthraxin averaged 37.9% (12.01 ÷ 78.9%). Immunological examination of individuals three weeks (21 days) after vaccination against anthrax revealed CAST-positivity in all the vaccinated persons. Intensity of anthraxin-induced basophil activation the vaccinated subjects was ranged from 10.87 to 30.03%, averaging 17.86%. The overall values of spontaneous and specific activation ranged within 12.39 ÷ 41.46%. The study opens prospectives for implementation of basophil antigenic activation test in the Flow CAST format in diagnostics of anthrax and to identify specific immune rearrangements after vaccination in humans, as an index of actual vaccination rates. Usage of CAST test with anthraxin makes it possible to identify anthrax patients at the early stages (2-4 days after onset of the disease) including, among patients with an increased CCR3+CD63+ background values, evaluation of immunological efficiency in the cohorts at risk for vaccination. At the same time, it was found that a significant decrease in diagnostic sensitivity of CAST test could be observed in the patients immune to anthrax pathogen who received intensive antibacterial and pathogenetic therapy at the early stages of infection, including glucocorticosteroids (anti-inflammatory drugs) and desensitizing agents that inhibit the degree of hypersensitivity development and its expression

IgG4-RELATED DISEASE. CLINICAL NOTES

IgG4-related diseases are a new nosological entity that encompasses a few previously known diseases. IgG4-related systemic disease is diagnosed if two or more affected organs are detected. This group of diseases has two similar signs: serological (elevated serum IgG4 subclass concentrations) and histological (organ and tissue infiltration from plasmo-cytes secreting IgG4, and eosinophils, and the development of fibrosclerosis and phlebitis obliterans). The paper describes two cases. In one case, a multisystemic disease was observed virtually at its onset whereas in the other this lesion was diagnosed several years after the natural course of the disease