SEQUENCING AND COMPARATIVE ANALYSIS OF THE MITOCHONDRIAL CYTOCHROME B GENE IN THE MARION UPLANDS FLORIDA SAND SKINK (PLESTIODON REYNOLDSI)

The Florida sand skink (Plestiodon reynoldsi)—a small (~10 cm) lizard endemic to the peninsula—is a ‘sand-swimming’ specialist restricted to Florida scrub habitat on the state’s central highland ridges. Florida scrub has been severely fragmented through urban growth and citrus farming, and less than 10% of this ecosystem remains. Given the skink’s limited geographic range and extensive population fragmentation, P. reynoldsi was listed as a federally threatened species in 1987. I surveyed skink populations from the Marion Uplands, where suitable lizard habitat is naturally (and has been historically) isolated from scrub on nearby Mt. Dora and Lake Wales ridges. I wanted to determine genetic relatedness of Marion Uplands skinks to those inhabiting these two ridges and hypothesized that Marion populations should be more similar genetically to those on the Mt. Dora ridge, given their geographic proximity. Mitochondrial DNA sequence analysis confirmed this hypothesis but also revealed unexpectedly high levels of genetic divergence between the Marion and Mt. Dora populations. Indeed, observed genetic divergence was comparable to that detected between Marion and Lake Wales populations

Presentation, Clinical Pathology Abnormalities, and Identification of Gastrointestinal Parasites in Camels (Camelus bactrianus and Camelus dromedarius) Presenting to Two North American Veterinary Teaching Hospitals. A Retrospective Study: 1980–2020

Old World Camelids (OWC) represent two species (Camelus bactrianus and Camelus dromedarius) with increasing numbers in North America. Gastrointestinal (GI) parasitism is a major cause of clinical disease in camelids and leads to significant economic impacts. Literature reporting on clinical parasitism of camels is localized to India, Africa, and the Middle East, with limited information available on OWCs in North America. Objectives of this study were to report on clinical presentation and diagnostic findings in Camelus bactrianus and Camelus dromedarius with GI parasitism and provide a comparative analysis between geographic regions. Medical records of OWCs presenting to two veterinary teaching hospitals (of the University of Tennessee and University of Wisconsin) were evaluated. Thirty-one camels including 11 Bactrians and six dromedaries (14 species not recorded) were included for the clinical component of this study, reporting on signalment, presenting complaint, and clinical pathology. Anorexia, weight loss, and diarrhea were the most common presenting complaint. Clinical pathology findings included eosinophilia, hypoproteinemia, and hyponatremia. For the second component of this study, a total of 77 fecal parasite examination results were evaluated for parasite identification and regional variation. Trichuris, Capillaria, Strongyloides, Nematodirus, Dictyocaulus, Moniezia, and protozoan parasites (Eimeria, Cryptosporidium, Giardia) were recorded. Strongyle-type eggs predominated, followed by Trichuris and Eimeria spp. There was a statistically significant variation in prevalence of coccidia between the two regions, with fecal examinations from Tennessee more likely to contain Eimeria (P = 0.0193). Clinicians treating camels in North America should recognize anorexia, weight loss, and diarrhea combined with clinical pathologic changes of hypoproteinemia, eosinophilia and hyponatremia as possible indications of GI parasitism. Clinicians should also consider the potential for regional variation to exist for GI parasites of camels in different areas of North America

SEQUENCING AND COMPARATIVE ANALYSIS OF THE MITOCHONDRIAL CYTOCHROME B GENE IN THE MARION UPLANDS FLORIDA SAND SKINK (PLESTIODON REYNOLDSI)

The Florida sand skink (Plestiodon reynoldsi)—a small (~10 cm) lizard endemic to the peninsula—is a ‘sand-swimming’ specialist restricted to Florida scrub habitat on the state’s central highland ridges. Florida scrub has been severely fragmented through urban growth and citrus farming, and less than 10% of this ecosystem remains. Given the skink’s limited geographic range and extensive population fragmentation, P. reynoldsi was listed as a federally threatened species in 1987. I surveyed skink populations from the Marion Uplands, where suitable lizard habitat is naturally (and has been historically) isolated from scrub on nearby Mt. Dora and Lake Wales ridges. I wanted to determine genetic relatedness of Marion Uplands skinks to those inhabiting these two ridges and hypothesized that Marion populations should be more similar genetically to those on the Mt. Dora ridge, given their geographic proximity. Mitochondrial DNA sequence analysis confirmed this hypothesis but also revealed unexpectedly high levels of genetic divergence between the Marion and Mt. Dora populations. Indeed, observed genetic divergence was comparable to that detected between Marion and Lake Wales populations

Presentation, Clinical Pathology Abnormalities, and Identification of Gastrointestinal Parasites in Camels (Camelus bactrianus and Camelus dromedarius) Presenting to Two North American Veterinary Teaching Hospitals. A Retrospective Study: 1980–2020

Old World Camelids (OWC) represent two species (Camelus bactrianus and Camelus dromedarius) with increasing numbers in North America. Gastrointestinal (GI) parasitism is a major cause of clinical disease in camelids and leads to significant economic impacts. Literature reporting on clinical parasitism of camels is localized to India, Africa, and the Middle East, with limited information available on OWCs in North America. Objectives of this study were to report on clinical presentation and diagnostic findings in Camelus bactrianus and Camelus dromedarius with GI parasitism and provide a comparative analysis between geographic regions. Medical records of OWCs presenting to two veterinary teaching hospitals (of the University of Tennessee and University of Wisconsin) were evaluated. Thirty-one camels including 11 Bactrians and six dromedaries (14 species not recorded) were included for the clinical component of this study, reporting on signalment, presenting complaint, and clinical pathology. Anorexia, weight loss, and diarrhea were the most common presenting complaint. Clinical pathology findings included eosinophilia, hypoproteinemia, and hyponatremia. For the second component of this study, a total of 77 fecal parasite examination results were evaluated for parasite identification and regional variation. Trichuris, Capillaria, Strongyloides, Nematodirus, Dictyocaulus, Moniezia, and protozoan parasites (Eimeria, Cryptosporidium, Giardia) were recorded. Strongyle-type eggs predominated, followed by Trichuris and Eimeria spp. There was a statistically significant variation in prevalence of coccidia between the two regions, with fecal examinations from Tennessee more likely to contain Eimeria (P = 0.0193). Clinicians treating camels in North America should recognize anorexia, weight loss, and diarrhea combined with clinical pathologic changes of hypoproteinemia, eosinophilia and hyponatremia as possible indications of GI parasitism. Clinicians should also consider the potential for regional variation to exist for GI parasites of camels in different areas of North America.This article is published as Locklear TR, Videla R, Breuer RM, Mulon P-Y, Passmore M, Mochel JP, Gerhold R, Schaefer JJ and Smith JS, "Presentation, Clinical Pathology Abnormalities, and Identification of Gastrointestinal Parasites in Camels (Camelus bactrianus and Camelus dromedarius) Presenting to Two North American Veterinary Teaching Hospitals. A Retrospective Study: 1980–2020." Frontiers in Veterinary Science 8 (2021):651672. DOI: 10.3389/fvets.2021.651672. Copyright 2021 Locklear, Videla, Breuer, Mulon, Passmore, Mochel, Gerhold, Schaefer and Smith. Attribution 4.0 International (CC BY 4.0). Posted with permission