Direct observation of significant hot carrier cooling suppression in a two-dimensional silicon phononic crystal

Finding hot carrier cooling suppression in new material structures is fundamentally important for developing promising technological applications. These phenomenona have not been reported for crystalline silicon phononic crystals. Herein, we experimentally design two-dimensional (2D) silicon samples consisting of airy hole arrays in a crystalline silicon matrix. For reference, the determined hot carrier cooling times were 0.45 ps and 0.37 ps, respectively, at probe wavelengths of 1080 nm and 1100 nm. Surprisingly, when the 2D structured silicon possessed the properties of a phononic crystal, significant suppression of hot carrier cooling was observed. In these cases, the observed hot carrier cooling times were as long as 15.9 ps and 10.7 ps at probe wavelengths of 1080 nm and 1100 nm, respectively, indicating prolongation by orders of magnitude. This remarkable enhancement was also observed with other probe wavelengths. The present work presents experimental evidence for hot carrier cooling suppression in 2D silicon phononic crystals and opens opportunities for promising applications

Super-multiplex vibrational imaging

The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have successfully been used to explore structure–function relationships in nervous systems, to profile RNA in situ, to reveal the heterogeneity of tumour microenvironments and to study dynamic macromolecular assembly, it remains challenging to image many species with high selectivity and sensitivity under biological conditions. For instance, fluorescence microscopy faces a ‘colour barrier’, owing to the intrinsically broad (about 1,500 inverse centimetres) and featureless nature of fluorescence spectra that limits the number of resolvable colours to two to five (or seven to nine if using complicated instrumentation and analysis). Spontaneous Raman microscopy probes vibrational transitions with much narrower resonances (peak width of about 10 inverse centimetres) and so does not suffer from this problem, but weak signals make many bio-imaging applications impossible. Although surface-enhanced Raman scattering offers high sensitivity and multiplicity, it cannot be readily used to image specific molecular targets quantitatively inside live cells. Here we use stimulated Raman scattering under electronic pre-resonance conditions to image target molecules inside living cells with very high vibrational selectivity and sensitivity (down to 250 nanomolar with a time constant of 1 millisecond). We create a palette of triple-bond-conjugated near-infrared dyes that each displays a single peak in the cell-silent Raman spectral window; when combined with available fluorescent probes, this palette provides 24 resolvable colours, with the potential for further expansion. Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type-dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this 24-colour (super-multiplex) optical imaging approach for elucidating intricate interactions in complex biological systems

Seismogenic structures and spatiotemporal seismicity patterns of the 2022 Ms6.0 Maerkang earthquake sequence, Sichuan, China

The 2022 Ms6.0 Maerkang earthquake sequence, Sichuan, China, occurred in an unexpected area with historically rare seismicity in the Bayan Har block. Here we relocated the earthquake sequence, inverted for the focal mechanisms of the larger events, and calculated the rupture directivity of the earthquake sequence to reveal the seismogenic structures and mechanisms of this sequence. The high-precision relocations indicate that the seismogenic structures consist of several clusters that are generally parallel to the nearby NW-trending Songgang fault, and relatively small-scale conjugate faults are also identified. The seismicity migrated from cluster one in the south to cluster two in the north during the sequence. Furthermore, the hypocenters were largely located at 5–10 km depth, thereby highlighting that the seismogenic structures are buried. The vertical fault planes of the seismogenic structures are consistent with the high-dip focal mechanism solutions from seven events. A stress field inversion based on the focal mechanisms indicates that the sequence occurred in a strike-slip environment that was controlled by a NNW–SSE-striking principal compressive stress. The different rupture directivities of the Ms5.8 (southwestward) and Ms6.0 (southeastward) events prove the existence of conjugate faults. The Ms5.8 event induced a coseismic Coulomb stress change of 1.6 MPa where the Ms6.0 event subsequently occurred, thereby highlighting that the Ms5.8 event triggered the Ms6.0 event and produced the spatiotemporal seismicity pattern of the sequence. We therefore conclude that the seismogenic structures of the 2022 Ms6.0 Maerkang earthquake sequence are previously unknown concealed conjugate structures associated with the main Songgang fault. The complex seismogenic structures and their potential to generate large earthquakes warrant the need to better understand the seismogenesis of this area and the seismic risks that may be present

Super-multiplex vibrational imaging

The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have successfully been used to explore structure–function relationships in nervous systems, to profile RNA in situ, to reveal the heterogeneity of tumour microenvironments and to study dynamic macromolecular assembly, it remains challenging to image many species with high selectivity and sensitivity under biological conditions. For instance, fluorescence microscopy faces a ‘colour barrier’, owing to the intrinsically broad (about 1,500 inverse centimetres) and featureless nature of fluorescence spectra that limits the number of resolvable colours to two to five (or seven to nine if using complicated instrumentation and analysis). Spontaneous Raman microscopy probes vibrational transitions with much narrower resonances (peak width of about 10 inverse centimetres) and so does not suffer from this problem, but weak signals make many bio-imaging applications impossible. Although surface-enhanced Raman scattering offers high sensitivity and multiplicity, it cannot be readily used to image specific molecular targets quantitatively inside live cells. Here we use stimulated Raman scattering under electronic pre-resonance conditions to image target molecules inside living cells with very high vibrational selectivity and sensitivity (down to 250 nanomolar with a time constant of 1 millisecond). We create a palette of triple-bond-conjugated near-infrared dyes that each displays a single peak in the cell-silent Raman spectral window; when combined with available fluorescent probes, this palette provides 24 resolvable colours, with the potential for further expansion. Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type-dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this 24-colour (super-multiplex) optical imaging approach for elucidating intricate interactions in complex biological systems

Food Security and Climate Stabilization: Can Cereal Production Systems Address Both?

There is abundant evidence that greenhouse gas (GHG) emissions of cereal products, expressed per ton of grain output, have been trending downward over the past 20 years. This has largely been achieved through agricultural intensification that has concurrently increased area-based GHG emissions. The challenge is for agriculture to increase grain yields to meet the food demands of a growing world population while also contributing to climate stabilization goals by reducing net GHG emissions. This study assessed yield-based and area-based emissions and efficiencies for the winter wheat–summer maize (WWSM) rotation system over the period 1996 to 2016 using long-term, longitudinal, farm survey data and detailed soil emission data in Huantai county, Shandong Province, which is an archetype for cereal production across the North China Plain (NCP). In this region, yields have been increasing over time. However, nitrogen fertilizer inputs have decreased substantially with greater adoption of soil nutrient testing. In addition, there has been widespread adoption of residue incorporation into soils. As such, since 2002, the product carbon footprints of wheat and maize have reduced by 25% and 30%, respectively. Meanwhile, area-based carbon footprints for the rotation system have reduced by around 15% over the same period. These findings demonstrate the importance of detailed assessment of soil N2O emissions and rates of soil organic carbon sequestration. They also show the potential for net reductions in GHG emissions in cropping without loss of grain yields