PO-225 The Effects on Mitochondria of Exercise Intervention PINK1 RNAi Drosophila Model of Parkinson's Disease Progression

Objective This research were using PINK1 RNAi drosophila model as object to  investigate the effects of the 3 weeks duration exercise intervention drosophila model of Parkinson’s disease progression. Including mitochondria gather situation and mitochondria complex I activity and climbing activity and wing posture. Trying to prove that Exercise-training can help with the Parkinson disease’s symptom, and also to find out the mechanism of the result.  Methods Two genotype were used in this research, the normal type VAS-mtGFPo  Mhc/Mhc and the Parkinson type VAS-mtGFP/Cyo Mhc-RNAi/TM2 and all with fluorescent labeling mitochondria by GFP. The exercise-training last for 3 weeks, 5 times per week, we separated the Parkinson type in 4 groups by different training time: 0min per day, 30min per day, 60min per day and 90min per day. The healthy type was control group. We measured the climbing ability and the wings posture every week, also used confocal microscope to watch the aggregation of mitochondria of the chest muscle every week. After 3 weeks training, we grinded all the drosophila to get the mitochondria, and measured the activity of mitochondria complex I. At last, we compared every index of each group with T-text by using SPSS 17.0 and the significance level as the criterion. Results 1）The climbing ability: No significant difference found in first week, there was a significant difference between 0min group and 30min group in second week and there was a very significant difference between 0min group and 30min group in third week. （P＜0.01）.2) Wing posture: There was a very significant difference between 0min group and 30min group in third week. （P＜0.01）. 3) Activity of mitochondria complex I: Because of the small sample of this experiment, we can’t get a appropriate concentration of mitochondria, but we still got some changes between 30min and 0min. 4) The pictures of mitochondria by confocal microscope: The 30min group has the least aggregations of the mitochondria but a lot aggregations were found in other pictures of other group.  Conclusions 1)The suitable  Exercise-training can improve the climbing ability and the account of wing posture and extend the life span of drosophila model of Parkinson’s disease. 2) The suitable   Exercise-training can relieve the situation of mitochondrial gathering and improve the activity of mitochondrial complex I.&nbsp

TRADITIONAL CHINESE MEDICINE ENDOTHERAPY OF DIABETIC WOUNDS: A SCOPING REVIEW

In recent years, the incidence of diabetes has been increasing, and the diabetic foot is a complication of diabetes, which seriously affects the life and health of patients. The effect of Chinese medicine in the treatment of diabetes is more and more recognized. To search for the treatment of diabetic foot using Traditional Chinese medicine - Just to collect research literature on TCM treatment of diabetes in order to summarize the common syndrome types and medication rules in recent years, and to provide a new treatment. Common syndrome has syndrome of the dual deficiency of qi and yin、syndrome of dual deficiency of qi and yin, syndrome of meridian stasis-heat, syndrome of meridian stasis-heat and so on. Common endotherapy medicine is clear heat medicinal, tonify qi medicine, activate blood and resolve stasis and so on. Traditional Chinese medicine has certain advantages and effects in the treatment of diabetes, but there is still no uniform standard.                                          Peer Review History: Received 20 February 2019;   Revised 2 March; Accepted 11 March, Available online 15 March 2020 Academic Editor: Dr. Ali Abdullah Al-yahawi, Al-Razi university, Department of Pharmacy, Yemen, [email protected] Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Nuray Ari, Ankara University, Turkiye, [email protected] Dr. Asia Selman Abdullah, Al-Razi university, Department of Pharmacy, Yemen, [email protected] Similar Articles: EFFECTS OF EMODIN ON BLOOD GLUCOSE AND BODY WEIGHT IN TYPE 1 DIABETIC RATS PLASMA FERRITIN AND HEPCIDIN LEVELS IN PATIENTS WITH TYPE 2 DIABETES MELLITU

How social distance affects the intention and behavior of collaborative consumption: a study based on online car-hailing service

In recent years, advances in mobile communications technology have enabled collaborative consumption or product sharing between consumers on a large scale. Unlike traditional consumption, collaborative consumption is based on collaboration among individuals, so that the decision-making mechanisms of individual consumers may be different from those in traditional consumption scenarios. The current study focuses on how the social distance between consumers and drivers affects collaborative consumption intention in the case of online car-hailing services. In this study, the theory of planned behavior (TPB) is used as the foundational framework, and we innovatively add the concept of social distance to the TPB to form a new, and integrated model. We test the model based on data collected from 315 online car-hailing users. The results shows that behavioral attitudes, subjective norms, and perceived behavioral control, positively influence collaborative consumption intention and behavior. More interestingly, we find that social distance has both direct and indirect impacts on collaborative consumption intention: The greater the social distance, the lower the collaborative consumption intention. Moreover, social distance also moderates the influence of subjective norms and perceived behavioral control on collaborative consumption intention. To be specific, the influence of subjective norms and perceived behavioral control on collaborative consumption intention is weakened when consumers perceive less social distance. The results suggest that the integrated model has a stronger explanatory power on collaborative consumption behavior. This study enhances the traditional TPB model and offers insight into promoting collaborative consumption in the context of the sharing economy

R.A.P.I.D. (Root Aggregated Prioritized Information Display): A single screen display for efficient digital triaging of medical reports

AbstractObjectiveThe timely acknowledgement of critical patient clinical reports is vital for the delivery of safe patient care. With current EHR systems, critical reports reside on different screens. This leads to treatment delays and inefficient work flows. As a remedy, the R.A.P.I.D. (Root Aggregated Prioritized Information Display) system represents all data on a single screen, and its simple and intuitive “button” array structure allows triaged sign-off/sign-out of critical and non-critical reports.Materials and methodsWith 100 hematology and chemistry reports from each of two EHR systems Meditech (Westwood, MA) and Orchard Labs, Inc. (Carmel, IN), we generated files of the reports in their individual standard display formats (enhanced Meditech-EM and enhanced Orchard-EO). We also displayed the same 200 reports in the R.A.P.I.D. format. We then conducted a randomized trial to compare the time and accuracy of acknowledgement of critical and non-critical results.ResultsThe sign-off times for reviewing the results for physician and non-physician providers, respectively, in seconds (with 95% confidence intervals) were for EM 1.78 (1.40–2.26) and 1.99 (1.72–2.30), for EO 2.69 (2.12–3.42) and 2.78 (2.40–3.21), and for R.A.P.I.D. 0.83 (0.70–0.98) and 1.58 (1.43–1.76). Non-physician providers reassigned system-defined non-critical results as critical with a frequency of 15.2% for EM, 18.4% for EO, and 7.83% for R.A.P.I.D., and critical results as non-critical with a frequency of 14.7%, 5.6%, and 5.8% respectively.DiscussionThe new display system was superior to two standard EHR systems that were significantly enhanced by first collecting the reports from their usual distributed locations and then by creating for each of the two standard EHRs a single file of reports for acknowledgement.ConclusionsFrom a single screen display of all reports, the new display system enables timely acknowledgement of critical reports for patient safety and non-critical report triage for improved provider work flows

Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells

Background: Recent evidence from rodent ovaries has demonstrated expression of fractalkine and the existence of fractalkine receptor, and showed that there is a significant increase in steroidogenesis in response to fractalkine, yet the role of fractalkine and CX3CR1 in the human ovary is still unknown. This study aimed to determine the expression levels of fractalkine and CX3CR1 in the human ovary and to investigate their roles in sexual hormone biosynthesis by human luteinising granulosa cells. This is the first detailed report of fractalkine and CX3CR1 expression and function in the human ovary. Methods: Fractalkine and CX3CR1 expression levels were measured by immunohistochemistry using ovarian tissue from pathological specimens from five individuals. Granulosa cells were obtained from patients during IVF treatment. They were cultured and treated with increasing doses of hCG with or without fractalkine. Media were collected to detect estradiol and progesterone by chemiluminescence. StAR, 3 beta HSD and CYP11A expression were determined in granulosa cells treated with or without fractalkine by real-time RT-PCR. Results: Fractalkine and CX3CR1 were expressed in the human ovary and in luteinising granulosa cells. However, fractalkine expression was stronger in luteinising granulosa cells. Treatment with fractalkine augmented hCG stimulation of progesterone production in a dose-dependent manner with concomitant increases in transcript levels for key steroidogenic enzymes (StAR, 3-beta HSD and CYP11A) but had no effect on estradiol biosynthesis(P < 0.05). Conclusions: Fractalkine and CX3CR1 were found to express in human ovary and luteinising granulosa cells. Fractalkine can increase the biosynthesis of progesterone in a dose-dependent manner by enhancing transcript levels of key steroidogenic enzymes.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292939500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Endocrinology & MetabolismReproductive BiologySCI(E)PubMed2ARTICLE95

Molecular characterization of Listeria monocytogenes strains isolated from imported food in China from 14 countries/regions, 2003-2018

Listeria monocytogenes (Lm) is associated with severe foodborne infections and ubiquitous in the nature. Identification of characteristics of Lm transmission through trading of food products is essential for rapidly tracking Lm sources and controlling dissemination of listeriosis. In this study, a total of 44 Lm strains were isolated from food products originating from 14 countries/regions during 2003-2018 at the Shanghai port. The genomes of these Lm strains were sequenced by high-throughput sequencing. Multilocus sequence typing (MLST) analysis showed that 43 isolates were divided into 17 sequence types (STs). The distribution of STs was decentralized, with the dominant ST2 accounting for only 18.18% of the strains. The LM63 strain did not match with any of the existing STs. Core-genome MLST (cgMLST) analysis based on 1748 core genes categorized the 44 strains into 30 cgMLST types (CTs), with CT10153 and CT7892 as the most predominant CTs. Notably, LM63 and LM67 shared the same CT in the cgMLST analysis. The phylogenetic analysis based on single-copy homologous genes revealed that the 44 Lm strains were primarily classified into two lineages. The SNP analysis also indicated that these strains were roughly divided into two clades, with strains in the first clade mainly collected earlier than those in the second clade, which were predominantly collected from 2010 onwards. The analysis using the virulence factor database (VFDB) indicated that the virulence gene inlJ was the most prevalent among these 44 strains. Notably, ddrA, msbA, and sugC were enriched in this dataset, requiring further clarification of their roles in Listeria through future studies. These results might provide a clue for understanding of the global epidemiology and surveillance of Lm and present insights for implementing effective measures to reduce or prevent Listeria contamination outbreaks in imported food products

The ratio between digestible protein and digestible energy affects accumulation and depuration of geosmin and 2-methylisoborneol (2-MIB) in Japanese seabass (Lateolabrax japonicus) raised in a recirculated aquaculture system

acceptedVersio

Long-term efficacy of non-steroid immunosuppressive agents in anti-muscle-specific kinase positive myasthenia gravis patients: a prospective study

Background and Purpose: Anti-muscle-specific kinase (MuSK) positive myasthenia gravis (MG) is characterized by a high relapsing rate, thus, choosing the appropriate oral drug regimen is a challenge. This study aimed to evaluate the efficacy of oral immunosuppressants (IS) in preventing relapse in MuSK-MG. Methods: This prospective cohort observational study included patients with MuSK-MG at Peking Union Medical College Hospital between January 1, 2018, and November 15, 2021. The patients were divided into 2 groups: those with (IS+) or without (IS-) non-steroid immunosuppressive agents. The primary outcome was relapsed at follow-up, and the log-rank test was used to compare the proportion of maintenance-free relapse between the groups; hazard ratio (HR) was calculated using the Cox proportional hazards models. Results: Fifty-three of 59 patients with MuSK-MG were included in the cohort, 14 were in the IS+ group, and 39 were in the IS- group. Twenty-four cases in the cohort experienced relapse at least once; the relapse rate was 2/14 (14.3%) in the IS+ group and 22/39 (56.4%) in the IS- group. At the end of follow-up, the proportion of maintenance-free relapse was significantly different between the two groups (log-rank χ2 = 4.94, P = 0.02). Of all the potential confounders, only the use of IS was associated with a reduced risk of relapse. The HR for relapse among patients in the IS+ group was 0.21 (95%CI 0.05–0.58) and was 0.23 (95%CI 0.05–0.93) in a model adjusted for age, sex, relapse history, highest Myasthenia Gravis Foundation of America (MGFA), and accumulated time of steroid therapy. Conclusions: This study provides evidence that oral non-steroid immunosuppressive agents may be beneficial in reducing relapse in patients with MuSK-MG