34 research outputs found
Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot
Sera from 88 patients from Santa Catarina and SĂŁo Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy.Amostras de soro de 88 pacientes dos Estados de Santa Catarina e SĂŁo Paulo, Brasil, com crises epilĂ©pticas e que se submeteram a exame de Tomografia Computadorizada (TC), foram examinadas para detecção de anticorpos anti-cisticercos de Taenia solium por meio de ELISA e Immunoblot (IB) utilizando-se os seguintes antĂgenos: extrato salino total de cisticercos de T. solium (Tso); lĂquido vesicular de Taenia crassiceps (Tcra-vf) e glicoproteĂnas purificadas de cisticercos de T. crassiceps (Tcra-gp). Os resultados de ELISA com os antĂgenos Tso, Tcra-vf e Tcra-gp mostraram 95%, 90% e 80% de sensibilidade, respectivamente, e 68%, 85% e 93% de especificidade, respectivamente. No grupo de pacientes epilĂ©pticos, a positividade do ELISA foi 30%, 51% e 35% com os antĂgenos Tso, Tcra-vf e Tcra-gp, respectivamente. Considerando o IB como teste confirmatĂłrio, a positividade foi de 16% (14/88) no grupo total de pacientes epilĂ©pticos e 22% (12/54) no grupo de pacientes epilĂ©pticos com TC positiva e sinais clĂnicos compatĂveis com neurocisticercose. Foi encontrada correlação estatĂstica significativa entre os resultados de ELISA ou IB e a fase da doença com quaisquer dos antĂgenos utilizados (p < 0,05). Os resultados indicam a necessidade de introduzir na rotina dos laboratĂłrios o diagnĂłstico de neurocisticercose nos pacientes com convulsĂ”es epilĂ©pticas devido ao elevado risco de aquisição da cisticercose em nossa regiĂŁo e sua participação na etiologia da epilepsia
IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis
Taenia antigens detection in the cerebrospinal fluid of patients with neurocysticercosis and its relationship with clinical activity of the disease
Passive haemagglutination test for human neurocysticercosis immunodiagnosis: II - Comparison of two standardized procedures for the passive haemagglutination reagent in the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluids
ImunodiagnĂłstico da neurocisticercose: teste imunoenzimĂĄtico com antĂgenos quimicamente ligados a suportes para pesquisa de anticorpos em soro e lĂquido cefalorraquiano
Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens
2 nd Brazilian Consensus on Chagas Disease, 2015
Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research