An Enhanced Electroosmotic Micromixer with an Efficient Asymmetric Lateral Structure

Homogeneous and rapid mixing in microfluidic devices is difficult to accomplish, owing to the low Reynolds number associated with most flows in microfluidic channels. Here, an efficient electroosmotic micromixer based on a carefully designed lateral structure is demonstrated. The electroosmotic flow in this mixer with an asymmetrical structure induces enhanced disturbance in the micro channel, helping the fluid streams’ folding and stretching, thereby enabling appreciable mixing. Quantitative analysis of the mixing efficiency with respect to the potential applied and the flow rate suggests that the electroosmotic microfluidic mixer developed in the present work can achieve efficient mixing with low applied potential

Mixing Mechanism of Microfluidic Mixer with Staggered Virtual Electrode Based on Light-Actuated AC Electroosmosis

In this paper, we present a novel microfluidic mixer with staggered virtual electrode based on light-actuated AC electroosmosis (LACE). We solve the coupled system of the flow field described by Navier–Stokes equations, the described electric field by a Laplace equation, and the concentration field described by a convection–diffusion equation via a finite-element method (FEM). Moreover, we study the distribution of the flow, electric, and concentration fields in the microchannel, and reveal the generating mechanism of the rotating vortex on the cross-section of the microchannel and the mixing mechanism of the fluid sample. We also explore the influence of several key geometric parameters such as the length, width, and spacing of the virtual electrode, and the height of the microchannel on mixing performance; the relatively optimal mixer structure is thus obtained. The current micromixer provides a favorable fluid-mixing method based on an optical virtual electrode, and could promote the comprehensive integration of functions in modern microfluidic-analysis systems

The Influence of Electric Field Intensity and Particle Length on the Electrokinetic Transport of Cylindrical Particles Passing through Nanopore

The electric transport of nanoparticles passing through nanopores leads to a change in the ion current, which is essential for the detection technology of DNA sequencing and protein determination. In order to further illustrate the electrokinetic transport mechanism of particles passing through nanopores, a fully coupled continuum model is constructed by using the arbitrary Lagrangian–Eulerian (ALE) method. The model consists of the electric field described by the Poisson equation, the concentration field described by Nernst–Planck equation, and the flow field described by the Navier–Stokes equation. Based on this model, the influence of imposed electric field and particle length on the electrokinetic transport of cylindrical particles is investigated. It is found firstly the translation velocities for the longer particles remain constant when they locate inside the nanopore. Both the ion current blockade effect and the ion current enhancement effect occur when cylindrical particles enter and exit the nanopore, respectively, for the experimental parameters employed in this research. Moreover, the particle translation velocity and current fluctuation amplitude are dominated by the electric field intensity, which can be used to adjust the particle transmission efficiency and the ion current detectability. In addition, the increase in particle length changes the particle position corresponding to the peak value of the ion current, which contributes to distinguishing particles with different lengths as well

Numerical Investigation of DC Dielectrophoretic Deformable Particle–Particle Interactions and Assembly

In a non-uniform electric field, the surface charge of the deformable particle is polarized, resulting in the dielectrophoretic force acting on the surface of the particle, which causes the electrophoresis. Due to dielectrophoretic force, the two deformable particles approach each other, and distort the flow field between them, which cause the hydrodynamic force correspondingly. The dielectrophoresis (DEP) force and the hydrodynamic force together form the net force acting on the particles. In this paper, based on a thin electric double layer (EDL) assumption, we developed a mathematical model under the arbitrary Lagrangian–Eulerian (ALE) numerical approach method to simulate the flow field, electric field, and deformable particles simultaneously. Simulation results show that, when two deformable particles’ distances are in a certain range, no matter the initial position of the two particles immersed in the fluid field, the particles will eventually form a particle–particle chain parallel to the direction of the electric field. In actual experiments, the biological cells used are deformable. Compared with the previous study on the DEP motion of the rigid particles, the research conclusion of this paper provides a more rigorous reference for the design of microfluidics

Fish Assemblage Responses to a Low-head Dam Removal in the Lancang River

Dam removal is becoming an effective approach for aquatic biodiversity restoration in damming river in order to balance the aquatic ecosystem conservation with large-scale cascade damming. However, the effects of dam removal on fish communities in Asian mountainous rivers, which are dominated by Cypriniformes fishes, are still not well known. To determine whether dam removal on a mountainous river benefit restoration of fish diversity, we investigated the response of fish assemblage to dam removal using a before- after-control-impact design in two tributaries of the Lancang River (dam removal river: the Jidu River, and control river: the Fengdian River). Fish surveys were conducted one year prior to dam removal (2012) and three years (2013-2015) following dam removal. We observed rapidly and notably spatio-temporal changes in fish biodiversity metrics and assemblage structure, occurring in the Jidu River within the first year after dam removal. Overall, fish species richness, density and Shannon-Wiener diversity all increased immediately in above- and below-dam sites, and maintained a stable level in subsequent years, compared to unchanged situation in the control river. All sites in the Jidu River experienced shifts in fish composition after dam removal, with the greatest temporal changes occurred in sites below- and above- the former dam, resulting in a temporal homogenization tendency in the dam removed river. These findings suggest that dam removal can benefit the recovery of habitat conditions and fish community in Asian mountainous rivers, but the results should be further evaluated when apply to other dammed rivers since the dam age, fluvial geomorphology and situation of source populations could all affect the responses of fish assemblages