10 research outputs found
Indium Tin Oxide Thin-Film Thermocouple Probe Based on Sapphire Microrod
Indium tin oxide (ITO) thin-film thermocouples monitor the temperature of hot section components in gas turbines. As an in situ measuring technology, the main challenge of a thin-film thermocouple is its installation on complex geometric surfaces. In this study, an ITO thin-film thermocouple probe based on a sapphire microrod was used to access narrow areas. The performance of the probe, i.e., the thermoelectricity and stability, was analyzed. This novel sensor resolves the installation difficulties of thin-film devices
A Through-Hole Lead Connection Method for Thin-Film Thermocouples on Turbine Blades
To solve the current problems with thin-film thermocouple signals on turbine blades in ultra-high temperature environments, this study explores the use of a through-hole lead connection technology for high-temperature resistant nickel alloys. The technique includes through-hole processing, insulation layer preparation, and filling and fixing of a high-temperature resistant conductive paste. The through-hole lead connection preparation process was optimized by investigating the influence of the inner diameter of the through-hole, solder volume, and temperature treatment on the contact strength and surface roughness of the thin-film for contact resistance. Finally, the technology was combined with a thin-film thermocouple to perform multiple thermal cycling experiments on the surface of the turbine blade at a temperature of 1000 °C. The results show that the through-hole lead connection technology can achieve a stable output of the thin-film thermocouple signal on the turbine blade
Diagram summarizing our findings miR-130b inversely target both <i>PGC-1α</i> and <i>PPAR-γ</i> in DCMECs.
<p>The down regulation of miR-130b increases <i>PGC-1α</i> and <i>PPAR-γ</i>expression levels in DCMECs.</p
Expression of fat metabolism related genes.
<p>GMECs were transfected with miR-130b mimic or inhibitor for 48h, the mRNA expression of <i>ACSL</i>,<i>CPT</i>, <i>ACOX1</i>, <i>HSL</i>, <i>ATGL</i>, <i>SCD</i>, <i>DGAT1</i>, <i>LPL</i>, <i>CD36</i>, <i>SLA27A6</i>, <i>SREBP1 and PPARγ</i> were quantified by RT-qPCR (n = 6). White bars, negative control; black bars, miR-130b mimic or inhibitor. All experiments were performed in duplicate and repeated three times. Values are presented as means ± standard errors, *, <i>P</i><0.05; **, <i>P</i><0.01.</p
miR-130b suppresses Lipid metabolism in GMECs.
<p>A: miR-130bmimic treatment (60nM); B: miR-130b inhibitor treatment (60nM); C: Triglyceride levels in cells transfected with miR-130b mimic or inhibitor; Triglyceride levels were compared with that of control (n = 6). White bars, negative control; black bars, miR-130b mimic or inhibitor. D: Cell proliferation levels in GMECs. White bars, negative control; black bars, miR-130b mimic or inhibitor. All experiments were performed in duplicate and repeated three times. Values are presented as means ± standard errors, *, <i>P</i><0.05; **, <i>P</i><0.01.</p
Fat droplet detection after transecting with miR-130b mimic or inhibitor in GMEC.
<p>Changes in the lipid contents of GMECs transfected with miR-130b mimic (60nM) or miR-130b inhibitor (60nM). Cells were stained by Oil Red. After examined microscopically, the oil red O was extracted with 400 μl of isopropanol and its absorbance was determined at 510 nm. The relative fat droplet content was normalized to control transfected cells. A: NC mimic treatment, B: miR-130b mimic treatment, C: NC inhibior treatment, D: miR-130b inhibior treatment. E: fat droplet content in cells. Fat droplet was compared with that of control (n = 6). White bars, negative control; black bars, miR-130b mimic or inhibitor. All experiments were performed in duplicate and repeated three times. Values are presented as means ± standard errors, *, <i>P</i><0.05; **, <i>P</i><0.01.</p
PGC-1α was identified as a target of miR-130b in GMECs.
<p>A: GMECs were transfected with miR-130b mimic or inhibitor for 48h, <i>PGC-1α</i> expression level was quantified by RT-qPCR (n = 4). White bars, negative control; black bars, miR-130b mimic or inhibitor. B and E: Target site of miR-130b in <i>PGC-1α</i> 3’UTR and the construction of the luciferase (Luc) expression vector fused with the <i>PGC-1α</i> 3’UTR. WT, Luc reporter vector with the WT <i>PGC-1α</i> 3’UTR (1958 to 1981); MU, Luc reporter vector with the mutation at miR-130b site in <i>PGC-1α</i> 3’UTR. C: Western blot analysis of <i>PGC-1α</i> expression in the miR-130b mimic and NC treatment experiments. The effect of miR-130b mimics on PGC-1α protein expression was evaluated by western blot analysis in GMECs. Total protein was harvested 24 h or 18 h post-transfection, respectively. NC, negative control. D: GMECs were transfected with siRNA of <i>PGC-1α</i> for 48h later, miR-130b expression levels was quantified by RT-qPCR (n = 6). All experiments were performed in duplicate and repeated three times. Values are presented as means ± standard errors, *, <i>P</i><0.05; **, <i>P</i><0.01</p
BoletÃn de Segovia: Número 30 - 1865 marzo 8
Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200