Design of Blended Teaching Model Based on Emotion Recognition and Language Learning

With the vigorous development of Internet technology, great changes have taken place in all aspects of human society. This change is also having an increasingly significant impact on the education sector. It is even a trend to subvert the tradition. This also makes the student's identity a passive recipient of knowledge. At the same time, the final orientation of China's education model is the result of examinations, and there is little guidance for students' interest. Of course, traditional teaching also has the ability to enable students' subject knowledge to be systematically established, and to communicate with teachers in the teaching process, which can improve students' learning efficiency in the classroom. In the face of the information explosion in today's society and the rapid development of the above-mentioned technologies, the change of students' learning mode of knowledge has also ushered in an opportunity to change. The purpose of this paper is to study the establishment of a blended teaching model that combines traditional classrooms with network applications. With the help of the characteristics of network big data, it transforms students' passive learning identities, and combines offline traditional learning classrooms with online learning. In addition to the advantages mentioned above, the advantage of online learning is that some network science and technology can be used for the online learning platform to serve the entire teaching process. Therefore, this paper proposes a blended teaching model based on the network platform for students' emotion recognition and language learning result analysis. And from the experimental results in this paper, it can be seen that the recognition evaluation rate of HTMC, the feature of emotion recognition, is 71.52% and the recognition frequency of ETMC is 73.89%. The above two recognition parameters can better reflect the emotional changes in the mixed teaching process

A Single‐Cell Perspective of the Mammalian Liver in Health and Disease

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154943/1/hep31149_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154943/2/hep31149.pd

Cloning and function analysis of DlWRKY9 gene in longan (Dimocarpus longan)

WRKY is one of the largest plant transcription factors (TFs) which is widely involved in plant growth, development, and responses to stresses. In the present study, a WRKY TF DlWRKY9 was cloned from longan (Dimocarpus longan). The coding sequence (CDS) of DlWRKY9 is 762 bp in length and encodes 253 amino acids. It has a typical WRKY domain and zinc finger structure which belongs to type IIa WRKY protein. The molecular weight of DlWRKY9 protein was 30.27kda and the theoretical isoelectric point (PI) was 5.24. It is an unstable hydrophilic protein. The secondary structure of DlWRKY9 protein consists of helical structure (17.39%), extended chain (8.70%) and other structures (turn and random coil) (73.91%). The amino acid sequence of DlWRKY9 protein had the highest similarity with DlWRKY9 (xp_006450293.1) of citrus Clementina. DlWRKY9 gene promoter elements contain light, abscisic acid, gibberellin, jasmonic acid and other response elements. The results of qRT-PCR showed that the relative expression level of DlWRKY9 gene in pericarp was higher, followed by young fruits and floral organs. Meanwhile, DlWRKY9 gene specifically down-regulated in the early stage of flower induction in ‘Sijimi’ (SJ) longan. The results of transient expression of Arabidopsis protoplasts showed that the fluorescence signal was mainly concentrated in the nucleus. Moreover, overexpression of DlWRKY9 in Arabidopsis promoted early flowering. These results provide useful information for revealing the biological roles of DlWRKY9 in longan and increase our understanding of the WRKY family in fruit trees

Antisense inhibition of ATM gene enhances the radiosensitivity of head and neck squamous cell carcinoma in mice

<p>Abstract</p> <p>Background</p> <p>Treatment failure after radiotherapy of head and neck squamous cell carcinoma (HNSCC) could be a significant problem. Our objective is to sensitize SCCVII cells to ionizing radiation <it>in vitro </it>and <it>in vivo </it>through inhibiting ATM expression using antisense oligodeoxynucleotides (AS-ODNs), and investigate the potential mechanism of radiosensitization.</p> <p>Methods</p> <p>We designed and synthesized AS-ODNs that target ATM mRNA to reduce the ATM expression. The influence on the expression of ATM mRNA and protein in SCCVII cells were analysed by real-time quantitative PCR and western blotting respectively. Clonogenic survival assay was performed to detect the survival ability of SCCVII cells after irradiation, while flow cytometry used to analyse the cell cycle and apoptosis. The volume of solid tumors generated with SCCVII cells was measured, and cell apoptosis was analysed by TUNEL assay after irradiation.</p> <p>Results</p> <p>The relative ATM mRNA and protein expression in SCCVII cells treated with ATM AS-ODNs were decreased to 25.7 ± 3.1% and 24.1 ± 2.8% of that in untreated cells respectively (<it>P </it>< 0.05). After irradiation, the survival fraction (SF) of cells treated with ATM AS-ODNs was lower than that of other groups at the same dose of radiation (<it>P </it>< 0.05), while the percentage of cells in G2/M phase decreased and apoptotic rate of cells increased(<it>P </it>< 0.05). The inhibition rate in SCCVII cells solid tumor exposed to X-ray alone was 23.2 ± 2.7%, while it was 56.1 ± 3.8% in the group which irradiated in combination with the treatment of ATM AS-ODNs (<it>P </it>< 0.05). The apoptotic index for the group irradiated in combination with ATM AS-ODNs injection was 19.6 ± 3.2, which was significantly higher than that of others (<it>P </it>< 0.05)</p> <p>Conclusion</p> <p>Inhibition of ATM expression sensitized SCCVII cells to ionizing radiation <it>in vitro </it>and <it>in vivo</it>. The potential mechanism should be the defective G2/M cell cycle checkpoint control and enhanced radiation-induced apoptosis.</p

Combination of thymosin α1 with conventional therapy improves APC and IL-1R1 levels in children with severe pneumonia

Purpose: To investigate the effect of a combination of thymosin α1 with  conventional regimen on APC and IL-1R1 levels in children with severe pneumonia, and to provide a reference for its clinical treatment.Methods: A total of 96 children with severe pneumonia over a period of two years were divided into control and observation groups (48 cases per  group) by random number table method. The patients consisted of 46 males and 50 females within the age range of 0.1 - 6 years (mean age = 3.0 ± 1.2 years), and mean duration of 13.4 ± 2.2 days). Their acute physiology and chronic health evaluation II (APACHE II) was 20.3 ± 3.2 points. Patients in the control group were placed on conventional treatment programs, while those in the observation group, in addition to the  conventional treatments, received thymosin α1 (subcutaneous injection of thymosin α1 at a dose of 1.5 mg per injection) twice daily for the first 3 days, then once daily until the 7th day, after which the adverse reactions were observed and treated. Pulmonary function indices, and levels of APC, and IL-1R1 were also determined in the patients’ sera before and after the treatment regime.Results: The clinical efficacy of the observation group was significantly better (p &lt; 0.05) than that of control. There were no significant differences (p &gt; 0.05) in the indices of pulmonary function (maximal inspiratory and expiratory pressure, and peak expiratory flow) between the two groups before treatment. However, after treatment they were significantly higher (p &lt; 0.05) in the observation group than in control. There were no significant differences (p &gt; 0.05) in APC and IL-1R1 levels between the two groups before treatment, but after treatment, while the level of APC in the observation group was significantly increased (p &lt; 0.05), the IL-1R1 level was significantly decreased (p &lt; 0.05), when compared to the control group. Both groups showed no obvious adverse reactions during the treatmentregime.Conclusion: Combining thymosin α1 with routine treatment in the  management of children with severe pneumonia can significantly alleviate the symptoms of patients, greatly stimulate recovery of pulmonary function, improve APC and IL-1R1 levels, and prevent inflammation.Keywords: Pneumonia, Thymosin α1, Routine treatment, Inflammatory factors, Pulmonary functio

A Framing Link Based Tabu Search Algorithm for Large-Scale Multidepot Vehicle Routing Problems

A framing link (FL) based tabu search algorithm is proposed in this paper for a large-scale multidepot vehicle routing problem (LSMDVRP). Framing links are generated during continuous great optimization of current solutions and then taken as skeletons so as to improve optimal seeking ability, speed up the process of optimization, and obtain better results. Based on the comparison between pre- and postmutation routes in the current solution, different parts are extracted. In the current optimization period, links involved in the optimal solution are regarded as candidates to the FL base. Multiple optimization periods exist in the whole algorithm, and there are several potential FLs in each period. If the update condition is satisfied, the FL base is updated, new FLs are added into the current route, and the next period starts. Through adjusting the borderline of multidepot sharing area with dynamic parameters, the authors define candidate selection principles for three kinds of customer connections, respectively. Link split and the roulette approach are employed to choose FLs. 18 LSMDVRP instances in three groups are studied and new optimal solution values for nine of them are obtained, with higher computation speed and reliability

hnRNPU/TrkB Defines a Chromatin Accessibility Checkpoint for Liver Injury and Nonalcoholic Steatohepatitis Pathogenesis

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154982/1/hep30921-sup-0001-Supinfo.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154982/2/hep30921.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154982/3/hep30921_am.pd