Effect of space flight factors on alfalfa seeds

To explore the effect of space flight factors on the early development of alfalfa seedling, dry seeds were placed onboard a satellite for a 15-day flight. After retrieval, the ultra structure of seed coat and the chemical content of seed were tested, followed by tests for germinate ability, seedling growth, and mitotic and chromosome aberrations. Results showed that space flight factors have both positive and negative effects on alfalfa seeds. Positive effects include: (1) A 6.2% increase in germinate potential and (2) an 80% decrease in the number of hard seed in flight seeds. Meanwhile, negative effects included a decrease of 3.0 and 33.2% in the index of germination and vigor of flight seeds, respectively, which may be partly due to the inhibition of cell mitotic (26% less than ground control) and root growth (29.0% less than ground control) after the space flight. Moreover, the DNA and Ca2+ content of alfalfa seeds increased after the space flight, while the reserve energy content of alfalfa seeds, such as saccharine and fatty acid, decreased after the space flight. Conclusively, space flight factors accelerate the germination process of alfalfa seeds but restrain the root from growing due to chromosomal damage and abnormal mitosis induced by cosmic radiation.Key words: Alfalfa, space flight factors, germination, chromosome aberration

Mars Dust Storm Effects in the Ionosphere and Magnetosphere and Implications for Atmospheric Carbon Loss

Mars regional and global dust storms are able to impact the lower/upper atmospheres through dust aerosol radiative heating and cooling and atmospheric circulation. Here we present the first attempt to globally investigate how the dust impact transfers from the neutral upper atmosphere to the ionosphere and the induced magnetosphere above 100‐km altitude. This is achieved by running a multifluid magnetohydrodynamic model under nondusty and dusty atmospheric conditions for the 2017 late‐winter regional storm and the 1971–1972 global storm. Our results show that the dayside main ionospheric layer (below ∼250‐km altitude) undergoes an overall upwelling, where photochemical reactions dominate. The peak electron density remains unchanged, and the peak altitude shift is in accordance with the upper atmospheric expansion (∼5 and ∼15 km for the regional and global storms, respectively). Controlled by the day‐to‐night transport, the nightside ionosphere responds to the dust storms in a close connection with what happens on the dayside but not apparently with the ambient atmospheric change. At higher altitudes, dust‐induced perturbations propagate upward from the ionosphere to the magnetosphere and extend from the dayside to the nightside, within a broad region bounded by the induced magnetospheric boundary. It is found that the global dust storm is able to dramatically enhance the CO2+ loss by a factor of ∼3, which amounts to an increase of ∼20% or more for total carbon loss (in the forms of neutrals and ions). Strong dust storms are a potentially important factor in atmospheric evolution at Mars.Key PointsThe dayside main ionosphere is lifted in accordance with dust‐induced atmospheric expansion, with peak electron densities unchangedDust‐induced perturbations propagate upward from the ionosphere to the magnetosphere and extend from the dayside to the nightsideStrong dust storms may enhance CO2+ loss by a factor of ∼3 and increase total carbon loss (neutrals and ions) by ∼20% or morePeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154511/1/jgra55184_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154511/2/jgra-sup-0001-2019JA026838-Text_SI-S01.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154511/3/jgra55184.pd

Seven-level inverter with switched capacitors

The seven-level (7L) inverter with a nearly sinusoidal output voltage is suitable for renewable energy conversion applications. A novel 7L inverter topology with switched capacitors is proposed here. The topology is composed of an active clamping inverter and a switched-capacitor part in series connection. The switched-capacitor part, consisting of three switches and two clamped capacitors, can provide 1/3 or 2/3 level of output voltage. By connecting switched-capacitor part in forward or backward series to the inverter output, a 7L output voltage waveform can be obtained with enough redundancy switching states to balance the voltage of switching capacitors. A modified modulation method is also proposed to make the topology operating properly. The voltage of switched-capacitor part can be sustained E and 2E alternately by adjusting a modulation index according to the capacitor voltage and load current. The effectiveness of the proposed topology is validated by both simulative and experimental results. Comparing with conventional 7L inverter topology, less number of devices were adopted in the proposed topology with a simple and practicable modulation method

Investigational Microbiological Therapy for Glioma

Glioma is the most common primary malignancy of the central nervous system (CNS), and 50% of patients present with glioblastoma (GBM), which is the most aggressive type. Currently, the most popular therapies are progressive chemotherapy and treatment with temozolomide (TMZ), but the median survival of glioma patients is still low as a result of the emergence of drug resistance, so we urgently need to find new therapies. A growing number of studies have shown that the diversity, bioactivity, and manipulability of microorganisms make microbial therapy a promising approach for cancer treatment. However, the many studies on the research progress of microorganisms and their derivatives in the development and treatment of glioma are scattered, and nobody has yet provided a comprehensive summary of them. Therefore, in this paper, we review the research progress of microorganisms and their derivatives in the development and treatment of glioma and conclude that it is possible to treat glioma by exogenous microbial therapies and targeting the gut–brain axis. In this article, we discuss the prospects and pressing issues relating to these therapies with the aim of providing new ideas for the treatment of glioma

Evolution Analysis of the Aux/IAA Gene Family in Plants Shows Dual Origins and Variable Nuclear Localization Signals

The plant hormone auxin plays pivotal roles in many aspects of plant growth and development. The auxin/indole-3-acetic acid (Aux/IAA) gene family encodes short-lived nuclear proteins acting on auxin perception and signaling, but the evolutionary history of this gene family remains to be elucidated. In this study, the Aux/IAA gene family in 17 plant species covering all major lineages of plants is identified and analyzed by using multiple bioinformatics methods. A total of 434 Aux/IAA genes was found among these plant species, and the gene copy number ranges from three (Physcomitrella patens) to 63 (Glycine max). The phylogenetic analysis shows that the canonical Aux/IAA proteins can be generally divided into five major clades, and the origin of Aux/IAA proteins could be traced back to the common ancestor of land plants and green algae. Many truncated Aux/IAA proteins were found, and some of these truncated Aux/IAA proteins may be generated from the C-terminal truncation of auxin response factor (ARF) proteins. Our results indicate that tandem and segmental duplications play dominant roles for the expansion of the Aux/IAA gene family mainly under purifying selection. The putative nuclear localization signals (NLSs) in Aux/IAA proteins are conservative, and two kinds of new primordial bipartite NLSs in P. patens and Selaginella moellendorffii were discovered. Our findings not only give insights into the origin and expansion of the Aux/IAA gene family, but also provide a basis for understanding their functions during the course of evolution

Correction: Wentao Wu, et al. Evolution Analysis of the Aux/IAA Gene Family in Plants Shows Dual Origins and Variable Nuclear Localization Signals. Int. J. Mol. Sci. 2017, 18, 2107

The authors would like to insert some websites and citations in the following sentence, “First, the complete proteomes of these species were downloaded from the Phytozome website (Version 11; Available online: www.phytozome.org)” in the “Materials and Methods” section on page 13, paragraph 3.1 of their paper published in the International Journal of Molecular Sciences [1].[...

Distribution, Source Apportionment and Risk Assessment of Phthalate Esters in the Overlying Water of Baiyang Lake, China

As a kind of endocrine disruptor compounds, the presence of phthalate esters (PAEs) has become a global concern. In this study, the pollution levels and spatial distribution of sixteen PAEs were investigated. Their potential sources and eco-environmental health risk were discussed in Baiyang Lake and its upstream rivers during different periods. PAEs were detected in all of samples, ranging from 1215 to 3014 ng·L−1 in October 2020 and 1384 to 3399 ng·L−1 in May 2021. Dibutyl phthalate (DBP) and di-isobutyl phthalate (DIBP) were the predominant monomers, with a detection rate of 100% and the highest concentrations in the overlying water. Restricted by multiple factors, the spatial distribution difference between Baiyang Lake and its upstream rivers in October was more significant than in May. The source apportionment revealed that agricultural cultivation and disorderly use and disposal of plastic products were the primary factors for the contamination. The human health risk assessment indicated that eight PAE congeners did not pose significant carcinogenic and non-carcinogenic harms to males, females and children. However, the ecological risks of DBP, DIBP and di (2-ethylhexyl) phthalate to algae, crustaceans and fish species were moderate or high-risk levels. This study provides an appropriate dataset for the assessment of the pollution of PEs to the water ecosystem affected by anthropogenic activities