Normobaric hyperoxia protects the blood brain barrier through inhibiting Nox2 containing NADPH oxidase in ischemic stroke

Normobaric hyperoxia (NBO) has been shown to be neuro- and vaso-protective during ischemic stroke. However, the underlying mechanisms remain to be fully elucidated. Activation of NADPH oxidase critically contributes to ischemic brain damage via increase in ROS production. We herein tested the hypothesis that NBO protects the blood-brain barrier (BBB) via inhibiting gp91phox (or called Nox2) containing NADPH oxidase in a mouse model of middle cerebral artery occlusion (MCAO). Wild-type C57/BL6 mice and gp91phoxknockout mice were given NBO (95% O2) or normoxia (21% O2) during 90-min MCAO, followed by 22.5 hrs of reperfusion. BBB damage was quantified by measuring Evans blue extravasation. The protein levels of matrix metalloproteinase-9 (MMP-9), tight junction protein occludin and gp91phox were assessed with western blot. Gel zymography was used to assess the gelatinolytic activity of MMP-9. In the wild type mice, cerebral ischemia and reperfusion led to remarkable Evans blue extravasation, significantly increased gp91phox and MMP-9 levels and decreased occludin levels in the ischemic brain tissue. In gp91phox knockout mice, the changes in Evans blue extravasation, MMP-9 and occludin were at much smaller magnitudes when compared to the wild type. Importantly, NBO treatment significantly reduced the changes in all measured parameters in wild type mice, while did not cause additional reductions in these changes when gp91phox was knocked out. These results indicate that activation of Nox2 containing NADPH oxidase is implicated in the induction of MMP-9, loss of occludin and BBB disruption in ischemic stroke, and inhibition of Nox2 may be an important mechanism underlying NBO-afforded BBB protection

Nrdp1 Increases Ischemia Induced Primary Rat Cerebral Cortical Neurons and Pheochromocytoma Cells Apoptosis Via Downregulation of HIF-1α Protein

Neuregulin receptor degradation protein-1 (Nrdp1) is an E3 ubiquitin ligase that targets proteins for degradation and regulates cell growth, apoptosis and oxidative stress in various cell types. We have previously shown that Nrdp1 is implicated in ischemic cardiomyocyte death. In this study, we investigated the change of Nrdp1 expression in ischemic neurons and its role in ischemic neuronal injury. Primary rat cerebral cortical neurons and pheochromocytoma (PC12) cells were infected with adenoviral constructs expressing Nrdp1 gene or its siRNA before exposing to oxygen-glucose deprivation (OGD) treatment. Our data showed that Nrdp1 was upregulated in ischemic brain tissue 3 h after middle cerebral artery occlusion (MCAO) and in OGD-treated neurons. Of note, Nrdp1 overexpression by Ad-Nrdp1 enhanced OGD-induced neuron apoptosis, while knockdown of Nrdp1 with siRNA attenuated this effect, implicating a role of Nrdp1 in ischemic neuron injury. Moreover, Nrdp1 upregulation is accompanied by increased protein ubiquitylation and decreased protein levels of ubiquitin-specific protease 8 (USP8) in OGD-treated neurons, which led to a suppressed interaction between USP8 and HIF-1α and subsequently a reduction in HIF-1α protein accumulation in neurons under OGD conditions. In conclusion, our data support an important role of Nrdp1 upregulation in ischemic neuronal death, and suppressing the interaction between USP8 and HIF-1α and consequently the hypoxic adaptive response of neurons may account for this detrimental effect

Calibrating the absorption imaging of cold atoms under high magnetic fields

We develop a theoretical model for calibrating the absorption imaging of cold atoms under high magnetic fields. Comparing to zero or low magnetic fields, the efficiency of the absorption imaging becomes lower while it requires an additional correction factor to obtain the absolute atom number under the Beer-Lambert law. Our model is based on the rate equations and can account many experimental imperfections such as Zeeman level crossing, failures of hyperfine structures, off-resonant couplings, and low repumping efficiency, etc. Based on this method, we can precisely calculate the correction factor for atom number measurement without any empirical or fitting parameters. Meanwhile, we use a cold-atom apparatus of rubidium-85 to experimentally verify our model. Besides these, we find our work can also serve as a benchmark to measure the polarization impurity of a circular-polarized laser beam with high sensitivities. We believe this work will bring convenience for most of cold-atom experiments using absorption imaging.Comment: 9 pages, 5 figure

Protective effect of Yiguanjian decoction against DNA damage on concanavalin A-induced liver injury mice model

AbstractObjectiveTo investigate the inhibitory effect of Yiguanjian decoction (YD) on DNA damage in Concanavalin A (Con A)-induced liver injury mice model and to explain the possible mechanism.MethodsTotally 120 male BALB/c mice were randomly divided into 6 groups, 20 mice each: normal group, model group, Bifendate group, YD low dose group, YD middle dose group and YD high dose group. Except normal group, liver injury model induced by Con A was established. While modeling, each mouse in YD group was given YD (0.4 mL/20 g per day) by intragastric administration (0.13 g YD for YD low dose group; 0.26 g for YD middle dose group; 0.52 g for YD high dose group). Bifendate group was given Bifendate (0.2 g·kg−1·d−1) by gavage. Normal group and model group were fed with same volume of physiological saline daily. After 8 weeks, the serum alanine transaminase (ALT) and aspartate transaminase (AST) were tested. The hematoxylin-eosin staining was used to evaluate the grade of liver inflammation and liver fibrosis stage. Hepatocellular DNA damage was detected by single cell gel electrophoresis technology. The protein expression of tumor necrosis factor-α (TNF-α), Bax and MutT Homolog 1 (MTH1) was detected by western blotting and enzyme linked immunosorbent assay. Bax mRNA and MTH1 mRNA were detected by Real-time Polymerase Chain Reaction (PCR).ResultsYD can improve the degree of liver inflammation and fibrosis in the liver of chronic hepatitis mice, the dose effect relationship is remarkable (P < 0.05). YD can reduce liver cell DNA damage. The difference between YD middle dose group and model group was statistically significant (P < 0.05). YD middle dose group had decreased the protein expression of TNF-α in the mice liver of immunological liver injury (P < 0.05). YD can increase the protein expression of Bax (P < 0.05). Compared with normal group, the protein expression of MTH1 was decreased (P < 0.05), but there was no statistical significance between YD group and model group (P > 0.05). YD can increase the mRNA expression of Bax and MTH1 (both P < 0.05).ConclusionYD can effectively inhibit the DNA damage in immunological liver injury mice, the mechanism may be that it can decrease the TNF-α and increase the Bax and MTH1 expression

Social Interaction Patterns of the Disabled People in Asymmetric Social Dilemmas

The social participation of the disabled people is unsatisfactory and low, one of the reasons often overlooked but of great importance may lie in the disparate patterns of social interaction between the disabled people and the abled people. The current study respectively recruited 41 and 80 disabled people in two experiments and adopted give-some games and public good dilemma to explore social interaction patterns between the disabled abled people. The results were as follows: (1) the disabled people preferred to interact with the disabled people and the abled people preferred to interact with the abled people. (2) The disabled abled people had higher cooperation, satisfaction and sense of justice when interacting with the disabled people than interacting with the abled people. (3) Advantage in the number of the disabled people could reverse their disadvantage in the identity. These results are of important practical value, which provides related theoretical support for the disabled people’s federation and communities when carrying out activities for the disabled people

The Effect of the Irreversible Inequality on Pro-social Behaviors of People With Disabilities

Inequalities have always been central to psychology, sociology and related fields such as social policy, gender studies, critical race studies, and human geography. Although inequality affects pro-social behaviors, there are still some controversies over this issue among people with disabilities. The current study aimed to investigate pro-social behaviors of people with disabilities and the effect of the irreversible inequality on pro-social behaviors. A dictator game was used to explore the difference of pro-social behaviors between people with disabilities and people without disabilities, when facing intra- or inter-group members. The results showed that compared to people with disabilities, people without disabilities were likely to show more pro-social behaviors. People with disabilities preferred intra-group cooperation, while people without disabilities preferred inter-group cooperation. Indeed, the intra-group cooperation was significantly greater than the expected cooperation of the intra-group members for people with disabilities. When facing the inter-group members, people without disabilities showed more than expected, that others would cooperate with them. These findings indicated that social avoidance was a common phenomenon for people with disabilities in China, but the situation would be different when they faced different groups. In addition, irreversible inequality could influence individuals’ cooperative strategies when facing individuals in a different status

Reduced Intrinsic Non-Radiative Losses Allow Room-Temperature Triplet Emission from Purely Organic Emitters

Persistent luminescence from triplet excitons in organic molecules is rare, as fast non-radiative deactivation typically dominates over radiative transitions. This work demonstrates that the substitution of a hydrogen atom in a derivative of phenanthroimidazole with an N-phenyl ring can substantially stabilize the excited state. This stabilization converts an organic material without phosphorescence emission into a molecular system exhibiting efficient and ultralong afterglow phosphorescence at room temperature. Results from systematic photophysical investigations, kinetic modeling, excited-state dynamic modeling, and single-crystal structure analysis identify that the long-lived triplets originate from a reduction of intrinsic non-radiative molecular relaxations. Further modification of the N-phenyl ring with halogen atoms affects the afterglow lifetime and quantum yield. As a proof-of-concept, an anticounterfeiting device is demonstrated with a time-dependent Morse code feature for data encryption based on these emitters. A fundamental design principle is outlined to achieve long-lived and emissive triplet states by suppressing intrinsic non-radiative relaxations in the form of molecular vibrations or rotations

Evaluation of LAI Estimation of Mangrove Communities Using DLR and ELR Algorithms With UAV, Hyperspectral, and SAR Images

The high-precision estimation of mangrove leaf area index (LAI) using a deep learning regression algorithm (DLR) always requires a large amount of training sample data. However, it is difficult for LAI field measurements to collect a sufficient amount of sample data in mangrove wetlands. To tackle this challenge, this paper proposed an approach for expanding training samples and quantitatively evaluated the performance of estimating LAI for mangrove communities using Deep Neural Networks (DNN) and Transformer algorithms. This study also explored the effects of unmanned aerial vehicle (UAV) and Sentinel-2A multispectral, orbital hyper spectral (OHS), and GF-3 SAR images on LAI estimation of different mangrove communities. Finally, this paper evaluated the LAI estimation ability of mangrove communities using ensemble learning regression (ELR) and DLR algorithms. The results showed that: (1) the UAV images achieved the better LAI estimation of different mangrove communities (R2 = 0.5974–0.6186), and GF-3 SAR images were better for LAI estimation of Avicennia marina with high coverage (R2 = 0.567). The optimal spectral range for estimating LAI for mangroves in the optical images was between 650–680 nm. (2) The ELR model outperformed single base model, and produced the high-accuracy LAI estimation (R2 = 0.5266–0.713) for different mangrove communities. (3) The average accuracy (R2) of the ELR model was higher by 0.0019–0.149 than the DLR models, which demonstrated that the ELR model had a better capability (R2 = 0.5865–0.6416) in LAI estimation. The Transformer-based LAI estimation of A. marina (R2 = 0.6355) was better than the DNN model, while the DNN model produced higher accuracy for Kandelia candel (KC) (R2 = 0.5577). (4) With the increase in the expansion ratio of the training sample (10–50%), the LAI estimation accuracy (R2) of DNN and Transformer models for different mangrove communities increased by 0.1166–0.2037 and 0.1037–0.1644, respectively. Under the same estimation accuracy, the sample enhancement method in this paper could reduce the number of filed measurements by 20–40%

Intrinsic efficiency limits in low-bandgap non-fullerene acceptor organic solar cells

In bulk heterojunction (BHJ) organic solar cells (OSCs) both the electron affinity (EA) and ionization energy (IE) offsets at the donor–acceptor interface should equally control exciton dissociation. Here, we demonstrate that in low-bandgap non-fullerene acceptor (NFA) BHJs ultrafast donor-to-acceptor energy transfer precedes hole transfer from the acceptor to the donor and thus renders the EA offset virtually unimportant. Moreover, sizeable bulk IE offsets of about 0.5 eV are needed for efficient charge transfer and high internal quantum efficiencies, since energy level bending at the donor–NFA interface caused by the acceptors’ quadrupole moments prevents efficient exciton-to-charge-transfer state conversion at low IE offsets. The same bending, however, is the origin of the barrier-less charge transfer state to free charge conversion. Our results provide a comprehensive picture of the photophysics of NFA-based blends, and show that sizeable bulk IE offsets are essential to design efficient BHJ OSCs based on low-bandgap NFAs