Effects of different weaning strategies on survival and growth in Chinese longsnout catfish (Leiocassis longirostris Gunther) larvae

The effects of different weaning strategies during the larval rearing of Chinese longsnout catfish were determined in two trials. In the first trial, the effect of abrupt-weaning from live prey (Artemia nauplii) to micro-diet at 5, 6, 7, 8, 10 dph, respectively was investigated. The second trial examined the effect of weaning with co-feeding at different ages (6, 8 and 10 dph). The survival, growth, digestive enzymes, coefficient of variation of final body weight (CVFBW) and body length (CVBL), digestive enzyme activities, fish body lysozyme and fish body glucose were significantly influenced by abrupt-introducing of microdiet (P<0.05). When weaning with live prey, only the fish body lysozyme significantly increased in the group introduced to microdiet on 8 and 10 dph (P<0.05). The study showed that abrupt-weaning of Chinese longsnout catfish should be obtained after 10 dph. Co-feeding could reduce the stress to larvae and therefore the weaning could start at 6 dph with co-feeding. (C) 2012 Elsevier B.V. All rights reserved.The effects of different weaning strategies during the larval rearing of Chinese longsnout catfish were determined in two trials. In the first trial, the effect of abrupt-weaning from live prey (Artemia nauplii) to micro-diet at 5, 6, 7, 8, 10 dph, respectively was investigated. The second trial examined the effect of weaning with co-feeding at different ages (6, 8 and 10 dph)

Protection and Utilization of Chinese Giant Salamander Germplasm Resources

Chinese giant salamander (Andrias davidianus) is salamander germplasm resource that only distributed in China. It is the material basis of the evolution principle research, genetic research and artificial breeding. Since the 1990s, overharvesting, water pollution and habitat changes have seriously destroyed the germplasm resources of A. davidianus, their population density has decreased since then. Strengthening the protection and rational utilization of A. davidianus germplasm resources are key factors in impacting the healthy, stable and sustainable development of A. davidianus industry. Under the above background, we discussed the current problems with the protection for A. davidianus germplasm resources, put forward suggestions for the conservation of A. davidianus germplasm resources. It is expected to provide a reference to effective protection for A. davidianus germplasm resources, and provide a scientific foundation for further studies, so as to promote the high-quality development of related industry

Protection and Utilization of Chinese Giant Salamander Germplasm Resources

Chinese giant salamander (Andrias davidianus) is salamander germplasm resource that only distributed in China. It is the material basis of the evolution principle research, genetic research and artificial breeding. Since the 1990s, overharvesting, water pollution and habitat changes have seriously destroyed the germplasm resources of A. davidianus, their population density has decreased since then. Strengthening the protection and rational utilization of A. davidianus germplasm resources are key factors in impacting the healthy, stable and sustainable development of A. davidianus industry. Under the above background, we discussed the current problems with the protection for A. davidianus germplasm resources, put forward suggestions for the conservation of A. davidianus germplasm resources. It is expected to provide a reference to effective protection for A. davidianus germplasm resources, and provide a scientific foundation for further studies, so as to promote the high-quality development of related industry

Dataset on differential gene expression analysis for splenic transcriptome profiling and the transcripts related to six immune pathways in grass carp

The data presented in this paper are related to the research article entitled “Transcriptome profiling of developing spleen tissue and discovery of immune-related genes in grass carp (Ctenopharyngodon idella)” (Li et al. 2016) [1]. Please refer to this article for interpretation of the data. Data provided in this submission are comprised of the expression levels of unigenes, significantly differentially expressed genes(DEGs), significant enrichment GO term and KEGG pathway of DEGs, and information of the transcripts assigned to six immune pathways

Dataset on evolution analysis of splenic transcriptome in bighead carp and silver carp

Bighead carp (Aristichthys nobilis) and silver carp (Hypophthalmichthys molitrix) are closely related species in the subfamily Xenocypridinae within Cyprinidae, and they are also two of the four most important pond-cultured fish species in China. The ability to resist some diseases often differs significantly in silver carp and bighead carp during fishery production. However, the evolutionary divergence of the immune defense functions in these two species is still not understood at the molecular level. The data presented in this article are related to the research article entitled “Comparative analysis of spleen transcriptome detects differences in evolutionary adaptation of immune defense functions in bighead carp and silver carp” (Li et al., 2018). Please refer to this data article for interpretation of the data. Data provided in this submission comprise the Ka/Ks ratios of orthologs as well as adaptive evolution genes, expression levels of orthologs, and TPM value of genes expressed only in spleen of bighead carp or silver carp. These data provide a better understanding of the differences in evolutionary adaptation of immune defense functions in bighead carp and silver carp

Screening and Stability Analysis of Reference Genes for Gene Expression Normalization in Hybrid Yellow Catfish (Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂) Fed Diets Containing Different Soybean Meal Levels

In this study, we screened the expression stability of six reference genes (18S rRNA, β-actin, GAPDH, EF1a, B2M, and HPRT1) in hybrid yellow catfish (n = 6), considering the SBM levels, sampling time points, and different tissues. Four different statistical programs, BestKeeper, NormFinder, Genorm, and Delta Ct, combined with a method that comprehensively considered all results, were used to evaluate the expression stability of these reference genes systematically. The results showed that SBM levels significantly impacted the expression stability of most of the reference genes studied and that this impact was time-, dose-, and tissue-dependent. The expression stability of these six reference genes varied depending on tissue, sampling time point, and SBM dosage. Additionally, more variations were found among different tissues than among different SBM levels or sampling time points. Due to its high expression, 18S rRNA was excluded from the list of candidate reference genes. β-actin and GAPDH in the liver and β-actin, HPRT1 and EF1a in the intestine were the most stable reference genes when SBM levels were considered. HPRT1, and EF1a in tissues sampled at 2 W and EF1a and β-actin in tissues sampled at 4 and 6 W were proposed as two stable reference genes when different tissues were considered. When the sampling time points were considered, β-actin, EF1a, and HPRT1 were the top three stable reference genes in the intestine. In contrast, β-actin and B2M are the most stable reference genes in the liver. In summary, β-actin, EF1a, and HPRT1 were the more stable reference genes in this study. The stability of reference genes depends on the tissues, sampling time points, and SBM diet levels in hybrid yellow catfish. Therefore, attention should be paid to these factors before selecting suitable reference genes for normalizing the target genes