1,054 research outputs found
Disrupted seasonal clockwork in the population dynamics of a freshwater copepod by climate warming
Life history responses are expected to accompany climate warming, yet little is known how long-term effects of climate and environmental change affect the seasonal dynamics of planktonic organisms. We used an historical data set from Lake Washington (U.S.A.) to quantify population responses of a calanoid copepod (Leptodiaptomus ashlandi) to long-term changes in temperature and resource availability and explore potential mechanisms for the responses. Increasing water temperatures (annual mean increase of 1.5 degrees C in the upper 10-m water volume) and longer stratification periods (about 4 weeks) were observed between 1962 and 2005, coincident with a pronounced decline in Leptodiaptomus densities. However, production was maintained because of an increase in the production to biomass ratio and a life cycle shift in Leptodiaptomus from an annual to a 6-month cycle. Cross-wavelet analyses demonstrated that the annual thermal forcing of copepod recruitment observed during the first two decades of the study weakened substantially, leading to more stochastic population dynamics during the past two decades. This shift from one to two generations per year was most likely produced by a longer and warmer growing period combined with changing fluctuations in resource (phytoplankton) availability. Climate change can lead to higher-frequency voltinism in ectothermic organisms and to temporal reorganization of their population dynamics
Genetic and Neuroanatomical Support for Functional Brain Network Dynamics in Epilepsy
Focal epilepsy is a devastating neurological disorder that affects an
overwhelming number of patients worldwide, many of whom prove resistant to
medication. The efficacy of current innovative technologies for the treatment
of these patients has been stalled by the lack of accurate and effective
methods to fuse multimodal neuroimaging data to map anatomical targets driving
seizure dynamics. Here we propose a parsimonious model that explains how
large-scale anatomical networks and shared genetic constraints shape
inter-regional communication in focal epilepsy. In extensive ECoG recordings
acquired from a group of patients with medically refractory focal-onset
epilepsy, we find that ictal and preictal functional brain network dynamics can
be accurately predicted from features of brain anatomy and geometry, patterns
of white matter connectivity, and constraints complicit in patterns of gene
coexpression, all of which are conserved across healthy adult populations.
Moreover, we uncover evidence that markers of non-conserved architecture,
potentially driven by idiosyncratic pathology of single subjects, are most
prevalent in high frequency ictal dynamics and low frequency preictal dynamics.
Finally, we find that ictal dynamics are better predicted by white matter
features and more poorly predicted by geometry and genetic constraints than
preictal dynamics, suggesting that the functional brain network dynamics
manifest in seizures rely on - and may directly propagate along - underlying
white matter structure that is largely conserved across humans. Broadly, our
work offers insights into the generic architectural principles of the human
brain that impact seizure dynamics, and could be extended to further our
understanding, models, and predictions of subject-level pathology and response
to intervention
Simulation Study of Sulfonate Cluster Swelling in Ionomers
We have performed simulations to study how increasing humidity affects the
structure of Nafion-like ionomers under conditions of low sulfonate
concentration and low humidity. At the onset of membrane hydration, the
clusters split into smaller parts. These subsequently swell, but then maintain
constant the number of sulfonates per cluster. We find that the distribution of
water in low-sulfonate membranes depends strongly on the sulfonate
concentration. For a relatively low sulfonate concentration, nearly all the
side-chain terminal groups are within cluster formations, and the average water
loading per cluster matches the water content of membrane. However, for a
relatively higher sulfonate concentration the water-to-sulfonate ratio becomes
non-uniform. The clusters become wetter, while the inter-cluster bridges become
drier. We note the formation of unusual shells of water-rich material that
surround the sulfonate clusters.Comment: 24 pages, 15 figure
Arginine-rich peptides destabilize the plasma membrane, consistent with a pore formation translocation mechanism of cell-penetrating peptides
Recent molecular-dynamics simulations have suggested that the arginine-rich HIV Tat peptides translocate by destabilizing and inducing transient pores in phospholipid bilayers. In this pathway for peptide translocation, Arg residues play a fundamental role not only in the binding of the peptide to the surface of the membrane, but also in the destabilization and nucleation of transient pores across the bilayer. Here we present a molecular-dynamics simulation of a peptide composed of nine Args (Arg-9) that shows that this peptide follows the same translocation pathway previously found for the Tat peptide. We test experimentally the hypothesis that transient pores open by measuring ionic currents across phospholipid bilayers and cell membranes through the pores induced by Arg-9 peptides. We find that Arg-9 peptides, in the presence of an electrostatic potential gradient, induce ionic currents across planar phospholipid bilayers, as well as in cultured osteosarcoma cells and human smooth muscle cells. Our results suggest that the mechanism of action of Arg-9 peptides involves the creation of transient pores in lipid bilayers and cell membranes.Facultad de Ciencias Exacta
Life at high Deborah number
In many biological systems, microorganisms swim through complex polymeric
fluids, and usually deform the medium at a rate faster than the inverse fluid
relaxation time. We address the basic properties of such life at high Deborah
number analytically by considering the small-amplitude swimming of a body in an
arbitrary complex fluid. Using asymptotic analysis and differential geometry,
we show that for a given swimming gait, the time-averaged leading-order
swimming kinematics of the body can be expressed as an integral equation on the
solution to a series of simpler Newtonian problems. We then use our results to
demonstrate that Purcell's scallop theorem, which states that time-reversible
body motion cannot be used for locomotion in a Newtonian fluid, breaks down in
polymeric fluid environments
Propulsion in a viscoelastic fluid
Flagella beating in complex fluids are significantly influenced by
viscoelastic stresses. Relevant examples include the ciliary transport of
respiratory airway mucus and the motion of spermatozoa in the mucus-filled
female reproductive tract. We consider the simplest model of such propulsion
and transport in a complex fluid, a waving sheet of small amplitude free to
move in a polymeric fluid with a single relaxation time. We show that, compared
to self-propulsion in a Newtonian fluid occurring at a velocity U_N, the sheet
swims (or transports fluid) with velocity U / U_N = [1+De^2 (eta_s)/(eta)
]/[1+De^2], where eta_s is the viscosity of the Newtonian solvent, eta is the
zero-shear-rate viscosity of the polymeric fluid, and De is the Deborah number
for the wave motion, product of the wave frequency by the fluid relaxation
time. Similar expressions are derived for the rate of work of the sheet and the
mechanical efficiency of the motion. These results are shown to be independent
of the particular nonlinear constitutive equations chosen for the fluid, and
are valid for both waves of tangential and normal motion. The generalization to
more than one relaxation time is also provided. In stark contrast with the
Newtonian case, these calculations suggest that transport and locomotion in a
non-Newtonian fluid can be conveniently tuned without having to modify the
waving gait of the sheet but instead by passively modulating the material
properties of the liquid.Comment: 21 pages, 1 figur
Bioresorbable silicon electronics for transient spatiotemporal mapping of electrical activity from the cerebral cortex.
Bioresorbable silicon electronics technology offers unprecedented opportunities to deploy advanced implantable monitoring systems that eliminate risks, cost and discomfort associated with surgical extraction. Applications include postoperative monitoring and transient physiologic recording after percutaneous or minimally invasive placement of vascular, cardiac, orthopaedic, neural or other devices. We present an embodiment of these materials in both passive and actively addressed arrays of bioresorbable silicon electrodes with multiplexing capabilities, which record in vivo electrophysiological signals from the cortical surface and the subgaleal space. The devices detect normal physiologic and epileptiform activity, both in acute and chronic recordings. Comparative studies show sensor performance comparable to standard clinical systems and reduced tissue reactivity relative to conventional clinical electrocorticography (ECoG) electrodes. This technology offers general applicability in neural interfaces, with additional potential utility in treatment of disorders where transient monitoring and modulation of physiologic function, implant integrity and tissue recovery or regeneration are required
Simultaneous free-volume modeling of the self-diffusion coefficient and dynamic viscosity at high pressure
International audienceA free-volume model of the dynamic viscosity and the self-diffusion coefficients was discussed. The temperature-pressure variations of the dynamic viscosity and the self-diffusion coefficients of small molecules were predicted. The compounds, carbon tetrachloride, cyclohexane, benzene, chlorotrifluoromethane, tetramethylsilane and methylcyclohexane were used for the investigation. The relation between microstructure, free volume and different complex thermophysical properties were emphasized by the model
Evidence to support magnetic resonance conditional labelling of all pacemaker and defibrillator leads in patients with cardiac implantable electronic devices
Aims:
Many cardiac pacemakers and defibrillators are not approved by regulators for magnetic resonance imaging (MRI). Even following generator exchange to an approved magnetic resonance (MR)-conditional model, many systems remain classified ‘non-MR conditional’ due to the leads. This classification makes patient access to MRI challenging, but there is no evidence of increased clinical risk. We compared the effect of MRI on non-MR conditional and MR-conditional pacemaker and defibrillator leads. //
Methods and results:
Patients undergoing clinical 1.5T MRI with pacemakers and defibrillators in three centres over 5 years were included. Magnetic resonance imaging protocols were similar for MR-conditional and non-MR conditional systems. Devices were interrogated pre- and immediately post-scan, and at follow-up, and adverse clinical events recorded. Lead parameter changes peri-scan were stratified by MR-conditional labelling. A total of 1148 MRI examinations were performed in 970 patients (54% non-MR conditional systems, 39% defibrillators, 15% pacing-dependent) with 2268 leads. There were no lead-related adverse clinical events, and no clinically significant immediate or late lead parameter changes following MRI in either MR-conditional or non-MR conditional leads. Small reductions in atrial and right ventricular sensed amplitudes and impedances were similar between groups, with no difference in the proportion of leads with parameter changes greater than pre-defined thresholds (7.1%, 95% confidence interval: 6.1–8.3). //
Conclusions:
There was no increased risk of MRI in patients with non-MR conditional pacemaker or defibrillator leads when following recommended protocols. Standardizing MR conditions for all leads would significantly improve access to MRI by enabling patients to be scanned in non-specialist centres, with no discernible incremental risk
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