6 research outputs found
Gaschromatographische Bestimmung von Hydroxyprolin in biologischem Material des Menschen
Peer Reviewe
Loss of NKG2D in murine NK cells leads to increased perforin production upon long‐term stimulation with IL‐2
NK cells are innate lymphocytes responsible for lysis of pathogen-infected and transformed cells. One of the major activating receptors required for target cell recognition is
the NK group 2D (NKG2D) receptor. Numerous reports show the necessity of NKG2D for
effective tumor immune surveillance. Further studies identified NKG2D as a key element
allowing tumor immune escape. We here use a mouse model with restricted deletion
of NKG2D in mature NKp46+ cells (NKG2DNK). NKG2DNK NK cells develop normally,
have an unaltered IFN-γ production but kill tumor cell lines expressing NKG2D ligands (NKG2DLs) less efficiently. However, upon long-term stimulation with IL-2, NKG2Ddeficient NK cells show increased levels of the lytic molecule perforin. Thus, our findings
demonstrate a dual function of NKG2D for NK cell cytotoxicity; while NKG2D is a crucial
trigger for cytotoxicity of tumor cells expressing activating ligands it is also capable to
limit perforin production in IL-2 activated NK cells