Experimental Study for Optimizing Pedestrian Flows at Bottlenecks of Subway Stations

In subway stations, bottlenecks are the narrowed areas that reduce pedestrian flows in channels. Because pedestrians at bottlenecks are forced to dense together, bottlenecks decrease flow efficiency and pedestrians’ transfer comfort and may trigger serious crowd disasters such as trampling. This study used pedestrian experiments to investigate the methods of optimizing pedestrian traffic at bottlenecks of subway stations. Three optimization measures were proposed and evaluated by analyzing the characteristics of pedestrian flows, including efficiency, smoothness, and security. In this paper, setting the rear sides of the bottleneck entrance as straight and surface funnel shapes is called straight funnel shape and surface funnel shape, respectively. Setting a column at a bottleneck is called the column obstacle. The results showed that when efficiency or security come first, a column on the left is recommended; when comfort comes first, a concave funnel is recommended; when comprehensiveness is prioritized, a column on the left is recommended. Moreover, the larger the volume, the optimization is more obvious. Although many  bottlenecks cannot be prevented when subway stations are constructed, the proposed optimization measures may help ease their adverse effects by improving facility efficiency, smoothness, and security, and by providing recommendations for designing and managing subway stations.</p

Connectivity Contribution to Urban Hub Network Based on Super Network Theory – Case Study of Beijing

With the rapid development of urbanization in China, the number of travel modes and urban passenger transportation hubs has been increasing, gradually forming multi-level and multi-attribute transport hub networks in the cities. At the same time, Super Network Theory (SNT) has advantages in displaying the multi-layer transport hubs. The aim of this paper is to provide a new perspective to study connectivity contribution of potential hubs. Urban transport hubs are ranked through topological features based on Hub Super Network (HSN). This paper proposes two indexes based on Super-Edge (SE), Zero Hub Degree of SE (ZHDoSE) and a number of shared SEes (NSSE), respectively. Then, a case study was conducted in Beijing, which considers four combinations to study the influence of transport modes and subway lines on connectivity. The results show that no-normalization strengthens the contribution of transport modes and subway lines on connectivity. Besides, the transport mode contributes a lot to the connectivity. However, elements normalization strengthens the subway lines under ZHDoSE reciprocal. In addition, various weights of ZHDoSE and NSSE have different influences on the recognition results of SEes in HSN

Genetic effects of polymorphisms in candidate genes and the QTL region on chicken age at first egg

<p>Abstract</p> <p>Background</p> <p>The age at first egg (AFE), an important indicator for sexual maturation in female chickens, is controlled by polygenes. Based on our knowledge of reproductive physiology, 6 genes including gonadotrophin releasing hormone-I (<it>GnRH-I</it>), neuropeptide Y (<it>NPY</it>), dopamine D2 receptor (<it>DRD2</it>), vasoactive intestinal polypeptide (<it>VIP</it>), VIP receptor-1 (<it>VIPR-1</it>), and prolactin (<it>PRL</it>), were selected as candidates for influencing AFE. Additionally, the region between <it>ADL0201 </it>and <it>MCW0241 </it>of chromosome Z was chosen as the candidate QTL region according to some QTL databases. The objective of the present study was to investigate the effects of mutations in candidate genes and the QTL region on chicken AFE.</p> <p>Results</p> <p>Marker-trait association analysis of 8 mutations in those 6 genes in a Chinese native population found a highly significant association (P < 0.01) between <it>G840327C </it>of the <it>GnRH-I </it>gene with AFE, and it remained significant even with Bonferroni correction. Based on the results of the 2-tailed χ²test, mutations <it>T32742394C, T32742468C, G32742603A</it>, and <it>C33379782T </it>in the candidate QTL region of chromosome Z were selected for marker-trait association analysis. The haplotypes of <it>T32742394C </it>and <it>T32742468C </it>were significantly associated (P < 0.05) with AFE. Bioinformatics analysis indicated that <it>T32742394C </it>and <it>T32742468C </it>were located in the intron region of the SH3-domain GRB2-like 2 (<it>SH3GL2</it>) gene, which appeared to be associated in the endocytosis and development of the oocyte.</p> <p>Conclusion</p> <p>This study found that <it>G840327C </it>of the <it>GnRH-I </it>gene and the haplotypes of <it>T32742394C-T32742468C </it>of the <it>SH3GL2 </it>gene were associated with the chicken AFE.</p

The antioxidant activity of polysaccharides from Armillaria gallica

The purpose of this study was to investigate the antioxidant activity of Armillaria gallica polysaccharides. It explored whether Armillaria gallica polysaccharides (AgP) could prevent HepG2 cells from H2O2-induced oxidative damage. The results demonstrated that HepG2 cells were significantly protected by AgP, and efficiently suppressed the production of reactive oxygen species (ROS) in HepG2 cells. Additionally, AgP significantly decreased the abnormal leakage of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) caused by H2O2, protecting cell membrane integrity. It was discovered that AgP was also found to regulate the activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX), while reducing malondialdehyde (MDA), thus protecting cells from oxidative damage. According to the flow cytometry analysis and measurement of caspase-3, caspase-8, and caspase-9 activities, AgP could modulate apoptosis-related proteins and attenuate ROS-mediated cell apoptosis

Case Report: Step-by-step procedures for total intracorporeal laparoscopic kidney autotransplantation in a patient with distal high-risk upper tract urothelial carcinoma

A 47-year-old man presented to the emergency department with right abdominal pain and a new onset of painless haematuria two weeks earlier. Urine cytology test results suggested urothelial carcinoma. Computed tomography urography (CTU) showed a filling defect in the lower right ureter with right hydronephrosis. Lymphadenopathy and any signs of metastatic disease were absent on CTU. Cystoscopy appeared normal. Creatinine level was also normal before surgery. After the treatment options were discussed, the patient chose to undergo 3D total intracorporeal laparoscopic kidney autotransplantation, bladder cuff excision, and segmental resection of the proximal two-thirds of the ureter based on the membrane anatomy concept. After more than one year of follow-up, the patient was in good health and showed no signs of haematuria. Surveillance cystoscopy and CTU examination showed no evidence of disease recurrence. Therefore, it is reasonable to assume that kidney-sparing surgery may be considered for carefully selected patients with high-grade upper tract urothelial carcinoma

SNX14 deficiency-induced defective axonal mitochondrial transport in Purkinje cells underlies cerebellar ataxia and can be reversed by valproate

共济失调是一类以运动协调性紊乱为主要特征的神经系统症状，临床表现包括步态不稳、丧失平衡、吞咽困难、眼球运动异常、肌张力受损等。厦门大学医学院神经科学研究所王鑫教授团队首次从轴突线粒体运输这一全新视角揭示了一类遗传性共济失调的发病机制，并发现抗癫痫药--丙戊酸大幅度减缓模型小鼠的疾病进程，具有较强的转化应用价值，有望为共济失调提供新的治疗手段。 该研究工作由王鑫教授指导完成，厦门大学医学院助理教授张洪峰和博士生洪育娟共同完成主要实验工作。Loss-of-function mutations in SNX14 cause autosomal recessive spinocerebellar ataxia 20, which is a form of early-onset cerebellar ataxia that lacks molecular mechanisms and mouse models. We generated Snx14-deficient mouse models and observed severe motor deficits and cell-autonomous Purkinje cell degeneration. SNX14 deficiency disrupted microtubule organization and mitochondrial transport in axons by destabilizing the microtubule-severing enzyme spastin, which is implicated in dominant hereditary spastic paraplegia with cerebellar ataxia, and compromised axonal integrity and mitochondrial function. Axonal transport disruption and mitochondrial dysfunction further led to degeneration of high-energy-demanding Purkinje cells, which resulted in the pathogenesis of cerebellar ataxia. The antiepileptic drug valproate ameliorated motor deficits and cerebellar degeneration in Snx14-deficient mice via the restoration of mitochondrial transport and function in Purkinje cells. Our study revealed an unprecedented role for SNX14-dependent axonal transport in cerebellar ataxia, demonstrated the convergence of SNX14 and spastin in mitochondrial dysfunction, and suggests valproate as a potential therapeutic agent.We thank Tim Huang for helpful discussion, Wei Mo for sharing mouse lines, Li Zhong for sharing reagents, Aidong Han, Luming Yao, Caiming Wu, Mingxia Zhu, Qingfeng Liu, Lin Zhu, Shuo Zhang, Haiping Zheng, and Changchuan Xie for technical assistance, and Cui Li for providing bioinformatics software. We also thank Novogene Co., Ltd. and PTM Biolab Co., Ltd. for technical assistance in the transcriptomic and proteomic analyses, respectively. 厦门大学医学院许华曦、赵颖俊、张云武、杜丹教授在研究过程中给予大力帮助和支持。本研究工作得到国家重点研发计划项目、国家自然科学基金、福建省自然科学基金、厦门大学校长基金的资助和支持

A Dynamic Time Warping Algorithm Based Analysis of Pedestrian Shockwaves at Bottleneck

Since the quantitative methodology analysis of the high-density pedestrian shockwaves at a bottleneck is limited, this paper proposes a dynamic time warping (DTW) algorithm for identifying, analyzing, and verifying the shockwaves. A set of real-world trajectory data is used to illustrate the proposed algorithm. Results show that the DTW algorithm is capable of depicting the pedestrian shockwaves elaborately and accurately. Results also show that the shockwave velocity is unsteady, as throughout time the gathering wave velocity and the evanescent wave velocity are decreasing and increasing, respectively. The mutual influence between followers and leaders is decreased when the shockwave spreads. There is a linear relationship between the shockwave velocity and density. Furthermore, singularities present a potential match solution to help identify the changing of pedestrian behaviors. The DTW algorithm for evaluating the pedestrian system stability has significant intrinsic features in the pedestrian traffic control and management

The changes of surgical treatment for symptomatic uterine myomas in the past 15 Years

Study Objective: The aim of this study is to elaborate the changes of the surgical approach of treatment for uterine myomas in Yangpu Hospital in the past 15 years. Design: This was retrospective cohort study. Setting: Yangpu Hospital, Tongji University School of Medicine, Shanghai, China. Materials and Methods: A total of 4113 patients with symptomatic uterine myomas underwent surgical treatments. Interventions: Eight kinds of different surgeries were involved in the study, including abdominal or laparoscopic surgery, hysterectomy, or uterus-sparing myomectomy. Measurements: The study collected patients' clinical data and reviewed surgical access and approach, complications, and the results of following up. Results: A total of 1559 cases (37.9%) underwent uterus-sparing myomectomy, 3005 cases (73.1%) performed laparoscopic surgeries. The percentage of laparoscopic surgery was significantly higher than homochronous data of laparotomy after 2003 (P < 0.001). The per year total of uterus-reserved surgery was proved to be negatively correlated with patient's age (R2 = 0.930; P < 0.001). The rate of myomas recurrence was significantly lower in the combined myomectomy and uterine artery occlusion group (4%, 34/910) than in the single myomectomy group (10.5%, 44/420) (P < 0.001). Conclusions: Retaining uterus and minimally invasive surgery were the important trends of surgical treatment for symptomatic uterine myomas. Laparoscopic uterus-sparing myomectomy may be an alternative to hysterectomy to manage to appropriate patients with uterine myomas

Predictors of serological cure after penicillin therapy in HIV-negative patients with early syphilis in Shenzhen, China.

BackgroundSyphilis is a common infectious disease worldwide. Serological monitoring is important for syphilis management. We currently know little about the characteristics of this seronegative response. The aim of this study was to explore the factors associated with serological cure after treatment of early syphilis.MethodsA retrospective cohort study was conducted and the data of patients with early syphilis in a clinic in Shenzhen from 2011 to 2019 were retrieved. Univariable and multiple Cox proportional hazard regression models were utilized to identify factors associated with a serological cure state among syphilis patients with early syphilis two years after treatment.ResultsA total of 346 (85.9%) syphilis patients achieved serological cure. The multivariate analysis results revealed that having a baseline TRUST titer >1:8 was associated with an increased probability of serological cure, compared with having a baseline TRUST titer ≤1:8 (HR = 1.43, 95% CI = 1.10-1.85, PConclusionsTwo years after treatment, a higher percentage of early syphilis patients achieved serological cure. The study indicated that the syphilis stage and baseline serum titer were crucial factors associated with serological cure