Preliminary results of 45 patients with trigeminal neuralgia treated with radiosurgery compared to hypofractionated stereotactic radiotherapy, using a dedicated linear accelerator.

Abstract Radiosurgery (RS) and hypofractionated stereotactic radiotherapy (HSRT) were performed in 23 and 22 patients respectively for the treatment of trigeminal neuralgia. RS and HSRT were performed with a dedicated linear accelerator (LINAC): an invasive frame (for RS) or a relocatable stereotactic frame fitted with a thermoplastic mask and bite blocks (HSRT) were used for positioning patients. The RS treatment delivered 40 Gy in a single fraction, or for HSRT, the equivalent radiobiological fractionated dose - a total of 72 Gy in six fractions. The target (the retrogasserian cisternal portion of the trigeminal nerve) was identified by fusion of CT scans with 1-mm-thick T2-weighted MRI, and the radiant dose was delivered by a 10-mm-diameter cylindrical collimator. The results were evaluated using the Barrow Neurological Institute pain scale during follow-up (mean 3.9 years). The 95% isodose was applied to the entire target volume. After RS (23 patients), Class 1 results were observed in 10 patients; Class II in nine, Class IIIa in two, Class IIIb in one, and Class V results in one patient. Facial numbness occurred in two (8.7%) patients, and the trigeminal neuralgia recurred in two patients (8.7%). Following HSRT (22 patients), Class I results were achieved in eight patients, Class II in eight, Class IIIa in four, and Class IIIb in two patients; recurrence occurred in six (27.5%), and there were no complications. Thus, both RS and HSRT provided effective and safe therapy for the treatment of trigeminal neuralgia. Patients who underwent RS experienced better pain relief and a lower recurrence rate, whereas those who underwent HRST had no side effects, and in particular, no facial numbness

Facilitating the Child–Robot Interaction by Endowing the Robot with the Capability of Understanding the Child Engagement: The Case of Mio Amico Robot

AbstractSocial Robots (SRs) are substantially becoming part of modern society, given their frequent use in many areas of application including education, communication, assistance, and entertainment. The main challenge in human–robot interaction is in achieving human-like and affective interaction between the two groups. This study is aimed at endowing SRs with the capability of assessing the emotional state of the interlocutor, by analyzing his/her psychophysiological signals. The methodology is focused on remote evaluations of the subject's peripheral neuro-vegetative activity by means of thermal infrared imaging. The approach was developed and tested for a particularly challenging use case: the interaction between children and a commercial educational robot, Mio Amico Robot, produced by LiscianiGiochi©. The emotional state classified from the thermal signal analysis was compared to the emotional state recognized by a facial action coding system. The proposed approach was reliable and accurate and favored a personalized and improved interaction of children with SRs

In order to lower the antinutritional activity of serine protease inhibitors, we need to understand their role in seed development

DATA AVAILABILITY STATEMENT : The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author.Proteases, including serine proteases, are involved in the entire life cycle of plants. Proteases are controlled by protease inhibitors (PI) to limit any uncontrolled or harmful protease activity. The role of PIs in biotic and abiotic stress tolerance is well documented, however their role in various other plant processes has not been fully elucidated. Seed development is one such area that lack detailed work on the function of PIs despite the fact that this is a key process in the life cycle of the plant. Serine protease inhibitors (SPI) such as the Bowman- Birk inhibitors and Kunitz-type inhibitors, are abundant in legume seeds and act as antinutrients in humans and animals. Their role in seed development is not fully understood and present an interesting research target. Whether lowering the levels and activity of PIs, in order to lower the anti-nutrient levels in seed will affect the development of viable seed, remains an important question. Studies on the function of SPI in seed development are therefore required. In this Perspective paper, we provide an overview on the current knowledge of seed storage proteins, their degradation as well as on the serine protease-SPI system in seeds and what is known about the consequences when this system is modified. We discuss areas that require investigation. This includes the identification of seed specific SPIs; screening of germplasms, to identify plants with low seed inhibitor content, establishing serine protease-SPI ratios and lastly a focus on molecular techniques that can be used to modify seed SPI activity.NRF, South Africa incentive funding, was also partially supported by the ERA-NET co-funding on Food Systems and Climate (FOSC) BIO-BELIEF project.http://www.frontiersin.org/Plant_Scienceam2024Plant Production and Soil ScienceSDG-15:Life on lan

Antinutritional factors, nutritional improvement, and future food use of common beans : a perspective

Common bean seeds are an excellent source of protein as well as of carbohydrates, minerals, vitamins, and bioactive compounds reducing, when in the diet, the risks of diseases. The presence of bioactive compounds with antinutritional properties (e.g., phytic acid, lectins, raffinosaccharides, protease inhibitors) limits, however, the bean’s nutritional value and its wider use in food preparations. In the last decades, concerted efforts have been, therefore, made to develop new common bean genotypes with reduced antinutritional compounds by exploiting the natural genetic variability of common bean and also applying induced mutagenesis. However, possible negative, or positive, pleiotropic effects due to these modifications, in terms of plant performance in response to stresses or in the resulting technological properties of the developed mutant genotypes, have yet not been thoroughly investigated. The purpose of the perspective paper is to first highlight the current advances, which have been already made in mutant bean characterization. A view will be further provided on future research directions to specifically explore further advantages and disadvantages of these bean mutants, their potential use in innovative foods and representing a valuable genetic reservoir of combinations to assess the true functional role of specific seed bioactive components directly in the food matrix.The ERA-NET co-funding on Food Systems and Climate (FOSC) BIO-BELIEF project.https://www.frontiersin.org/journals/plant-sciencedm2022Plant Production and Soil Scienc

New polymeric hydrogel formulations for the controlled release of protein drugs

Combinations of partial esters of alternating maleic anhydride/mono-methoxyoligoethyleneglycol vinyl ethers copolymers with human serum albumin have been found suitable to give transparent, flexible and coherent films that display a marked hydrogel character and safely pass preliminary "in vitro" and "in vivo" toxicity tests. They have been used in the fabrication of polymeric inserts containing alpha-interferon (IFN(alpha)) by a low temperature casting procedure. Water uptake, dissolution levels, and kinetic release profiles of the active principle from inserts are presented and analyzed in terms of the different extent of hydrophilic interactions of proteins with the synthetic component in the hybrid polymer matrices