Application of three-dimensional technology in video-assisted thoracoscopic surgery sublobectomy

BackgroundDue to the widespread use of imaging techniques, the detection rate of early-stage lung cancer has increased. Video-assisted thoracoscopic surgery (VATS) sublobectomy has emerged as a prominent alternative to lobectomy, offering advantages like reduced resection range, better preservation of lung function, and enhanced postoperative quality of life. However, sublobectomy is more intricate than lobectomy, necessitating a higher level of surgical proficiency and anatomical understanding.MethodsThree electronic databases were searched to capture relevant studies from January 2016 to March 2023, which related to the application of three-dimensional(3D) technology in VATS sublobectomy.ResultsCurrently, clinical departments such as orthopedics, hepatobiliary surgery, and urology have started using 3D technology. This technology is expected to be widely used in thoracic surgery in future. Now 3D technology assists in preoperative planning, intraoperative navigation and doctor-patient communication.Conclusion3D technologies, instrumental in locating pulmonary nodules and identifying variations in target lung segmental vessels and bronchi, play pivotal roles in VATS sublobectomy, especially in preoperative planning, intraoperative navigation, and doctor-patient communication. The limitations of 3D technology in clinical application are analyzed, and the future direction of existing 3D technology development is prospected

Single-longitudinal-mode Fiber Ring Lasers With Taper-coupled Double-microsphere-cavities

This letter proposes and demonstrates a fiber ring laser using taper-coupled double-microsphere-cavities (DMC) to achieve single-longitudinal-mode operation. Whispering-gallery-mode (WGM) intensity distributions and transmission spectra of the DMC with different microsphere diameters are investigated both theoretically and experimentally. Due to the Vernier effect, the DMC can produce WGM spectra with a higher extinction ratio, a higher side-mode-suppression ratio (SMSR), a larger FSR and a narrower bandwidth, as compared to a single-microsphere cavity. A single-longitudinal-mode fiber ring laser operating near 1.5 μm with a bandwidth of < 0.01 nm and a signal-to-background ratio of about 60 dB is demonstrated using the taper-coupled DMC as an all-fiber mode selector

A universal delayed difference model fitting dose-response curves

Purpose: Dose-response curves, which fit a multitude of experimental data derived from toxicology, are widely used in physics, chemistry, biology, and other fields. Although there are many dose-response models for fitting dose-response curves, the application of these models is limited by many restrictions and lacks universality, so there is a need for a novel, universal dynamical model that can improve fits to various types of dose-response curves. Methods: We expand the hormetic Ricker model, taking the delay inherent in the dose-response into account, and develop a novel and dynamic delayed Ricker difference model (DRDM) to fit various types of dose-response curves. Furthermore, we compare the DRDM with other dose-response models to confirm that it can mimic different types of dose-response curves. Data analysis: By fitting various types of dose-response data sets derived from drug applications, disease treatment, pest control, and plant management, and comparing the imitative effect of the DRDM with other models, we find that the DRDM fits monotonic dose-response data well and, in most circumstances, the DRDM has a better imitative effect to non-monotonic dose-response data with hormesis than other models do. Results: The MSE of fits of the DRDM to S-shaped dose-response data (DS2-G) is not lower than those for four other models, but the MSE of fits to U-shaped (DS7) and inverted U-shaped dose-response data (DS10) were lower than for two other models. This means that the imitative effect of the DRDM is comparable to other models of monotonic dose-response data, but is a significant improvement compared to traditional models of non-monotonic dose-response data with hormesis. Conclusion: We propose a novel dynamic model (DRDM) for fitting to various types of dose-response curves, which can reflect the dynamic trend of the population growth compared with traditional static dose-response models. By analyzing data, we have confirmed that the DRDM provides an ideal description of various dose-response observations and it can be used to fit a wide range of dose-response data sets, especially for hormetic data sets. Therefore, we conclude that the DRDM has a good universality for dose-response curve fitting

New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina

<p>Abstract</p> <p>Background</p> <p>Subtilisin-like serine proteases play an important role in pathogenic fungi during the penetration and colonization of their hosts. In this study, we perform an evolutionary analysis of the subtilisin-like serine protease genes of subphylum Pezizomycotina to find if there are similar pathogenic mechanisms among the pathogenic fungi with different life styles, which utilize subtilisin-like serine proteases as virulence factors. Within Pezizomycotina, nematode-trapping fungi are unique because they capture soil nematodes using specialized trapping devices. Increasing evidence suggests subtilisin-like serine proteases from nematode-trapping fungi are involved in the penetration and digestion of nematode cuticles. Here we also conduct positive selection analysis on the subtilisin-like serine protease genes from nematode-trapping fungi.</p> <p>Results</p> <p>Phylogenetic analysis of 189 subtilisin-like serine protease genes from Pezizomycotina suggests five strongly-supported monophyletic clades. The subtilisin-like serine protease genes previously identified or presumed as endocellular proteases were clustered into one clade and diverged the earliest in the phylogeny. In addition, the cuticle-degrading protease genes from entomopathogenic and nematode-parasitic fungi were clustered together, indicating that they might have overlapping pathogenic mechanisms against insects and nematodes. Our experimental bioassays supported this conclusion. Interestingly, although they both function as cuticle-degrading proteases, the subtilisin-like serine protease genes from nematode-trapping fungi and nematode-parasitic fungi were not grouped together in the phylogenetic tree. Our evolutionary analysis revealed evidence for positive selection on the subtilisin-like serine protease genes of the nematode-trapping fungi.</p> <p>Conclusions</p> <p>Our study provides new insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina. Pezizomycotina subtilisins most likely evolved from endocellular to extracellular proteases. The entomopathogenic and nematode-parasitic fungi likely share similar properties in parasitism. In addition, our data provided better understanding about the duplications and subsequent functional divergence of subtilisin-like serine protease genes in Pezizomycotina. The evidence of positive selection detected in the subtilisin-like serine protease genes of nematode-trapping fungi in the present study suggests that the subtilisin-like serine proteases may have played important roles during the evolution of pathogenicity of nematode-trapping fungi against nematodes.</p

Spatiotemporal distribution and prediction of chlorophyll-a in Ulansuhai lake from an arid area of China

Lake Ulansuhai, a typical shallow lake in an arid area that is economically and ecologically important along the Yellow River, is currently eutrophic. Long-term (2010–2020) data on chlorophyll-a, nutrient, and environmental factors were obtained from three Lake Ulansuhai monitoring stations. The temporal and spatial distribution characteristics of Chl-a were analyzed. Additionally, a hybrid evolutionary algorithm was established to simulate and predict Chl-a, and sensitivity analysis revealed the interaction between environmental factors and eutrophication. The results indicated that (1) the seasonal variation of eutrophication showed an obvious trend of spring &gt; summer &gt; autumn &gt; winter, and the concentration of Chl-a in the inlet was significantly higher than that in the outlet; (2) The inlet, center, and outlet of Ulansuhai Lake are satisfactorily affected by HEA in the best suited method. The fitting coefficients (R2) of the optimal models were 0.58, 0.59, and 0.62 for the three monitoring stations, and the root mean square errors (RMSE) were 3.89, 3.21, and 3.56, respectively; (3) under certain range and threshold conditions, Chl-a increased with the increase of permanganate index, water temperature, dissolved oxygen concentration, and ammonia nitrogen concentration, but decreased with the increase of water depth, Secchi disk depth, pH, and fluoride concentration. The results indicate that the HEA can simulate and predict the dynamics of Chl-a, and identify and quantify the relationships between eutrophication and the threshold data. The research results provide theoretical basis and technical support for the prediction and have great significance for the improvement of water quality and environmental protection in arid and semi-arid inland lakes

Impacts of climate change and human activities on vegetation coverage variation in mountainous and hilly areas in Central South of Shandong Province based on tree-ring

IntroductionIt is of great significance to understand the characteristics and influencing factors of vegetation coverage variation in the warm temperate zone. As a typical region of the warm temperate zone in eastern China, the mountainous and hilly region in central-south Shandong Province has fragile ecological environment and soil erosion problem. Studying on vegetation dynamics and its influencing factors in this region will help to better understand the relationship between climate change and vegetation cover change in the warm temperate zone of eastern China, and the influence of human activities on vegetation cover dynamics.MethodsBased on dendrochronology, a standard tree-ring width chronology was established in the mountainous and hilly region of central-south Shandong Province, and the vegetation coverage from 1905 to 2020 was reconstructed to reveal the dynamic change characteristics of vegetation cover in this region. Secondly, the influence of climate factors and human activities on the dynamic change of vegetation cover was discussed through correlation analysis and residual analysis.Results and discussionIn the reconstructed sequence, 23 years had high vegetation coverage and 15 years had low vegetation coverage. After low-pass filtering, the vegetation coverage of 1911–1913, 1945–1951, 1958–1962, 1994–1996, and 2007–2011 was relatively high, while the vegetation coverage of 1925–1927, 1936–1942, 2001–2003, and 2019–2020 was relatively low. Although precipitation determined the variation of vegetation coverage in this study area, the impacts of human activities on the change of vegetation coverage in the past decades cannot be ignored. With the development of social economy and the acceleration of urbanization, the vegetation coverage declined. Since the beginning of the 21st century, ecological projects such as Grain-for-Green have increased the vegetation coverage

Tailoring electrolyte additives for advanced Mg-based anodes in primary aqueous Mg-air battery

This doctoral work proposes new approaches to boost discharge performance of aqueous Mg-air batteries (AMABs) via tailoring electrolyte additives for newly developed Mg-Ca anodes. Micro-alloyed Mg-Ca anode exhibits negative discharge potential and high utilization efficiency (UE) compared to other commercial Mg alloys. With the addition of suitable Mg2+ complexing agent into electrolyte, the discharge performance of Mg-Ca is able to be further improved. Therefore, the effect of Mg2+ complexing agents on the corrosion and discharge performance of Mg-Ca anode was investigated, indicating the potential of Mg2+ complexing agents to regulate the interfacial condition of the Mg anode/electrolyte and to enhance the specific energy of AMABs. In order to reveal the working mechanism of electrolyte additives, the evolution of interfacial condition between Mg anode and electrolyte was traced by electrochemical impedance spectroscopy. Nevertheless, the interpretation of impedance spectra has been still controversial, which may affect the accurate understanding of the additive working mechanism. Therefore, the physical interpretations of high frequency and middle frequency time constants in Mg impedance spectra were thoroughly investigated, revealing the high frequency time constant is originated form the barrier effect of surface film and the middle frequency time constant is related to charge transfer process and the electric double layer. Based on a better understanding about Mg impedance spectra, EIS measurements during discharge interval were applied to study the working mechanism of selected electrolyte additives. This work proposes two different types of electrolyte additives for AMABs based on a deep understanding of their working mechanisms, which would greatly facilitate the development and widespread applications of high-performance AMAB systems

Prenatal diagnosis and molecular cytogenetic analysis of a <i>de novo</i> isodicentric chromosome 18

Isodicentric chromosome 18 [idic(18)] is rare structural aberration. We report on a prenatal case described by conventional and molecular cytogenetic analyses. The sonography at 24 weeks of gestation revealed multiple fetal anomalies; radial aplasia and ventricular septal defect were significant features. Routine karyotyping showed a derivative chromosome replacing one normal chromosome 18. The parental karyotypes were normal, indicating that the derivative chromosome was <i>de novo</i>. Array comparative genomic hybridization (array-CGH) revealed 18p11.21&#8594;qter duplication and 18p11.21&#8594;pter deletion for genomic DNA of the fetus. The breakpoint was located at 18p11.21 (between 12104527 bp and 12145199 bp from the telomere of 18p). Thus, the derivative chromosome was ascertained as idic(18)(qter&#8594;p11.21::p11.21&#8594;qter). Fluorescent in situ hybridization (FISH) confirmed that the derivative chromosome was idic(18). Our report describes a rare isodicentric chromosome 18 and demonstrates that array-CGH is a useful complementary tool to cytogenetic analysis for reliable identifying derivative chromosome

Assessing Changes in the Landscape Pattern of Wetlands and Its Impact on the Value of Wetland Ecosystem Services in the Yellow River Basin, Inner Mongolia

The Yellow River Basin of Inner Mongolia has significant ecological advantages, and it is critical to research the landscape pattern of its watershed wetland ecosystem and the changes in its service value in order to protect the environment and develop the region in a high-quality manner. In this paper, we use the landscape index method, the equivalent factor method, and a field survey to investigate changes in wetland landscape patterns and the dynamics of wetland ecosystem service values in the Yellow River Basin of Inner Mongolia from 1990 to 2020, and then examine the impact of landscape pattern evolution on wetland ecosystem service values in the region. The study’s findings indicate that rivers, lakes, and herbaceous marshes are the most common types of wetland landscapes in Inner Mongolia’s Yellow River Basin. The landscape types in the research area are diverse, and landscape fragmentation is increasing. In the Yellow River Basin of Inner Mongolia, the overall value of wetland ecosystem benefits is negatively connected with Patch Density and the Shannon Diversity Index, and positively correlated with the Contagion Index

Energy Saving and Thermal Comfort Performance of Passive Retrofitting Measures for Traditional Rammed Earth House in Lingnan, China

The traditional rammed earth houses sharing similar patterns in the Lingnan region, south China, and distributed in rectangular arrays, are gradually losing their vitality and becoming uninhabited under modern living conditions. This research examined a typical pattern called the “Four-point gold” house and analyzed the suitability of different retrofitting technologies by field measurements and building simulation. To optimize energy consumption, indoor thermal comfort, and the corresponding economic performance of the retrofitting measures for the prototypical house, five measures, including wall insulation, reflective roof coating, carpet, sunshade, and natural ventilation, are proposed after considering the status quo of the building envelope. It is found that the best performance in energy-saving, dynamic investment payback period, and annual indoor thermal comfort are 2192.27 kWh/a, 9.17 years, and 1766 h, respectively. Different parameters are included to be clustered by K means clustering technique, and the comprehensively optimized scheme consists of a regime of 30 mm XPS 30 mm, ZS-221 white coating, carpet, 0.5 m sunshade width, and turning off windows (doors). The proposed retrofitting strategy can be promoted to a wide range of traditional rammed earth houses in the Lingnan region in China and holds a conspicuous energy-saving potential for the suburban and rural residential sectors in the region