Metagenomic insights into the abundance and composition of resistance genes in aquatic environments:Influence of stratification and geography

A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34x10(0) and 1.22x10(-3) -1.98x10(-1) copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multimetal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater

Uninterrupted CAG repeat drives striatum-selective transcriptionopathy and nuclear pathogenesis in human Huntingtin BAC mice

In Huntington's disease (HD), the uninterrupted CAG repeat length, but not the polyglutamine length, predicts disease onset. However, the underlying pathobiology remains unclear. Here, we developed bacterial artificial chromosome (BAC) transgenic mice expressing human mutant huntingtin (mHTT) with uninterrupted, and somatically unstable, CAG repeats that exhibit progressive disease-related phenotypes. Unlike prior mHTT transgenic models with stable, CAA-interrupted, polyglutamine-encoding repeats, BAC-CAG mice show robust striatum-selective nuclear inclusions and transcriptional dysregulation resembling those in murine huntingtin knockin models and HD patients. Importantly, the striatal transcriptionopathy in HD models is significantly correlated with their uninterrupted CAG repeat length but not polyglutamine length. Finally, among the pathogenic entities originating from mHTT genomic transgenes and only present or enriched in the uninterrupted CAG repeat model, somatic CAG repeat instability and nuclear mHTT aggregation are best correlated with early-onset striatum-selective molecular pathogenesis and locomotor and sleep deficits, while repeat RNA-associated pathologies and repeat-associated non-AUG (RAN) translation may play less selective or late pathogenic roles, respectively

Transport Accessibility and Poverty Alleviation in Guizhou Province of China: Spatiotemporal Pattern and Impact Analysis

Both transport development and poverty alleviation are vital for sustainable development. However, due to the lack of long-term, comparable, county-level transport accessibility and poverty incidence data, the spatiotemporal patterns of these factors have rarely been accurately revealed in the poverty-stricken regions of China, causing the impacts of transport accessibility on poverty alleviation to be difficult to quantify. Taking Guizhou Province in China as the study area, this study revealed the spatiotemporal patterns of transport accessibility and poverty alleviation in 88 counties from 2000 to 2018 based on multisource data, including nighttime light data, LandScan population data, and transport network data. It was found that the transport accessibility decreased from 4.9 h to 3.3 h, and the poverty index decreased from 0.75 to 0.29 on average. All these factors exhibited a &ldquo;core&ndash;periphery&rdquo; spatial pattern. Furthermore, the panel data regression analysis suggested that transport accessibility has played a dominant role in poverty alleviation, with an elasticity coefficient of 0.839. In the future, policies concerned to integrate transport development with rural industries such as agriculture, e-commence, and tourism are recommended for poverty alleviation and rural revitalization, which are especially significant for promoting sustainable development, securing a win&ndash;win of economic growth and social equity

Transport Accessibility and Poverty Alleviation in Guizhou Province of China: Spatiotemporal Pattern and Impact Analysis

Both transport development and poverty alleviation are vital for sustainable development. However, due to the lack of long-term, comparable, county-level transport accessibility and poverty incidence data, the spatiotemporal patterns of these factors have rarely been accurately revealed in the poverty-stricken regions of China, causing the impacts of transport accessibility on poverty alleviation to be difficult to quantify. Taking Guizhou Province in China as the study area, this study revealed the spatiotemporal patterns of transport accessibility and poverty alleviation in 88 counties from 2000 to 2018 based on multisource data, including nighttime light data, LandScan population data, and transport network data. It was found that the transport accessibility decreased from 4.9 h to 3.3 h, and the poverty index decreased from 0.75 to 0.29 on average. All these factors exhibited a “core–periphery” spatial pattern. Furthermore, the panel data regression analysis suggested that transport accessibility has played a dominant role in poverty alleviation, with an elasticity coefficient of 0.839. In the future, policies concerned to integrate transport development with rural industries such as agriculture, e-commence, and tourism are recommended for poverty alleviation and rural revitalization, which are especially significant for promoting sustainable development, securing a win–win of economic growth and social equity

Fundamental Properties of Nonlinear Stochastic Differential Equations

The existence of solutions is used the premise of discussing other properties of dynamic systems. The goal of this paper is to investigate the fundamental properties of nonlinear stochastic differential equations via the Khasminskii test, including the local existence and global existence of the solutions. Firstly, a fundamental result is given as a lemma to verify the local existence of solutions to the considered equation. Then, the equivalent proposition for the global existence and the fundamental principle for the Khasminskii test are formally established. Moreover, the classical Khasminskii test is generalized to the cases with high-order estimates and heavy nonlinearity for the stochastic derivatives of the Lyapunov functions. The role of the noise in this aspect is especially investigated, some concrete criteria are obtained, and an application for the role of the noise in the persistence of financial systems is accordingly provided. As another application of the fundamental principle, a new version of the Khasminskii test is established for the delayed stochastic systems. Finally the conclusions obtained in the paper are verified by simulation. The results show that, under weaker conditions, the global existence of better solutions to stochastic systems to those in the existing literature can be obtained

Building Integrated Photovoltaic (BIPV) Development Knowledge Map: A Review of Visual Analysis Using CiteSpace

Achieving zero energy consumption in buildings is one of the most effective ways of achieving &lsquo;carbon neutrality&rsquo; and contributing to a green and sustainable global development. Currently, BIPV systems are one of the main approaches to achieving zero energy in buildings in many countries. This paper presents the evolution of BIPV systems and predicts their future trends by deriving a base sample of core papers on BIPV systems from 2012 to 2022 from the Web of Science core database and conducting a bibliometric study using CiteSpace scientific visualisation software. To gain a deeper understanding and grasp of the research progress of BIPV systems, research group discovery, research hotspot analysis, and research frontier detection of the relevant literature were conducted. (1) Research groups on the topic were summarised through author coupling network, publication distribution, and country mapping analysis; (2) Research hotspots on the topic were explored through keyword co-occurrence, keyword emergence, and time zone map analysis; (3) Research hotspots on the topic were explored through literature co-citation timeline maps, literature co-citation categories, and literature co-citation clustering analysis to detect the frontiers of research in the field. Finally, we conclude that research trends in BIPV systems are mainly in the areas of heat transfer, thermal performance, renewable energy, solar cell and renewable building materials, and evaluation systems. In the future, BIPV research and applications will move towards interdisciplinary and multinational cooperation, which will maximise the benefits of clean energy conversion in buildings. It will also provide researchers and practitioners with a clearer understanding of BIPV research trends and hotspots, and provide new directions for future research

The association between early fluid strategy and prognosis of acute respiratory distress syndrome: A post hoc study of CHARDS

Abstract We aimed to assess general fluid management in China and evaluate the association between fluid balance and survival outcomes in acute respiratory distress syndrome (ARDS) patients. A retrospective, multicenter study including ARDS patients was conducted. We described the fluid management of ARDS patients in China. Furthermore, clinical characteristics and outcomes of patients subdivided by cumulative fluid balance were also analyzed. Multivariable logistic regression analysis was performed with hospital mortality as the outcome. From June 2016 to February 2018, 527 ARDS patients were included in our study. The mean cumulative fluid balance was 1669 (−1101 to 4351) mL in the first 7 day after intensive care unit (ICU) admission. Patients were divided into four groups based on cumulative fluid balance of the first 7 day after ICU admission: Group I (≤0 L), Group II (>0 L, ≤3 L), Group III (>3 L, ≤5 L), and Group IV (>5 L). Significantly lower hospital mortality was observed in patients with a lower cumulative fluid balance on day 7 of ICU admission (20.5% in Group I vs. 32.8% in Group II, 38.5% in Group III, and 50% in Group IV, p < 0.001). A lower fluid balance is associated with lower hospital mortality in patients with ARDS. However, a large‐scale and well‐designed randomized controlled trial is needed in the future

Stomatal Ratio Showing No Response to Light Intensity in <i>Oryza</i>

Stomata control carbon and water exchange between the leaves and the ambient. However, the plasticity responses of stomatal traits to growth conditions are still unclear, especially for monocot leaves. The current study investigated the leaf anatomical traits, stomatal morphological traits on both adaxial and abaxial leaf surfaces, and photosynthetic traits of Oryza leaves developed in two different growth conditions. Substantial variation exists across the Oryza species in leaf anatomy, stomatal traits, photosynthetic rate, and stomatal conductance. The abaxial stomatal density was higher than the adaxial stomatal density in all the species, and the stomatal ratios ranged from 0.35 to 0.46 across species in two growth environments. However, no difference in the stomatal ratio was observed between plants in the growth chamber and outdoors for a given species. Photosynthetic capacity, stomatal conductance, leaf width, major vein thickness, minor vein thickness, inter-vein distance, and stomatal pore width values for leaves grown outdoors were higher than those for plants grown in the growth chamber. Our results indicate that a broad set of leaf anatomical, stomatal, and photosynthetic traits of Oryza tend to shift together during plasticity to diverse growing conditions, but the previously projected sensitive trait, stomatal ratio, does not shape growth conditions

Effect of Misalignment between Successive Corneal Videokeratography Maps on the Repeatability of Topography Data.

To improve the reliability of corneal topographic data through the development of a method to estimate the magnitude of misalignment between successive corneal videokeratography (VK) maps and eliminate the effect of misalignment on the repeatability of topography data.Anterior and posterior topography maps were recorded twice for 124 healthy eyes of 124 participants using a Pentacam, and the repeatability of measurements was assessed by calculating the differences in elevation between each two sets of data. The repeatability of measurements was re-assessed following the determination of the magnitude of misalignment components (translational displacements: x0, y0 and z0, and rotational displacements: α, β and γ) between each two data sets and using them to modify the second data set within each pair based on an Iterative Closest Point (ICP) algorithm. The method simultaneously considered the anterior and posterior maps taken for the same eye since they were assumed to have the same set of misalignment components. A new parameter, named Combined Misalignment parameter (CM), has been developed to combine the effect of all six misalignment components on topography data and so enable study of the association between misalignment and the data repeatability test results.The repeatability tests resulted in average root mean square (RMS) differences in elevation data of 8.46±2.75 μm before ICP map matching when simultaneously considering anterior and posterior surfaces. With map matching and misalignment correction, the differences decreased to 7.28±2.58 μm (P = 0.00). When applied to only the anterior maps, misalignment correction led to a more pronounced reduction in elevation data differences from 4.58±1.84 μm to 2.97±1.29 μm (P = 0.00). CM was found to be associated with the repeatability error (P = 0.00), with posterior maps being responsible for most of the error due to their relatively lower accuracy compared to anterior maps.The ICP algorithm can be used to estimate, and effectively correct for, the potential misalignment between successive corneal videokeratography maps