Web Service for Creation of Informative Web Pages about Events

Tato bakalářská práce se zabývá analýzou technologií webových aplikací, dostupných vývojových prostředí, aplikačních rámců a v neposlední řadě také návrhem a vývojem webové služby. Tato služba svým uživatelům poskytuje možnost vytvořit informační webové stránky k pořádaným událostem soukromého charakteru. Služba je implementována v jazyce JavaScript. Na serverové straně je použit aplikační rámec Express, běhové prostředí Node.js a databáze MongoDB. Klientská část služby je implementována za použití aplikačního rámce React. This bachelor's thesis deals with analyzing technologies of web applications, available development environments, frameworks and last but not least also with designing and developing a web service. Such service provides creation of informational web pages about upcoming private events. The service is implemented in JavaScript. Express framework running in Node.js environment is used on the server-side. MongoDB is used as a database. Client-side is implemented with use of React framework.

The Impact of the Human DNA Topoisomerase II C-Terminal Domain on Activity

Type II DNA topoisomerases (topos) are essential enzymes needed for the resolution of topological problems that occur during DNA metabolic processes. Topos carry out an ATP-dependent strand passage reaction whereby one double helix is passed through a transient break in another. Humans have two topoII isoforms, alpha and beta, which while enzymatically similar are differentially expressed and regulated, and are thought to have different cellular roles. The C-terminal domain (CTD) of the enzyme has the most diversity, and has been implicated in regulation. We sought to investigate the impact of the CTD domain on activity.We have investigated the role of the human topoII C-terminal domain by creating constructs encoding C-terminally truncated recombinant topoIIalpha and beta and topoIIalpha+beta-tail and topoIIbeta+alpha-tail chimeric proteins. We then investigated function in vivo in a yeast system, and in vitro in activity assays. We find that the C-terminal domain of human topoII isoforms is needed for in vivo function of the enzyme, but not needed for cleavage activity. C-terminally truncated enzymes had similar strand passage activity to full length enzymes, but the presence of the opposite C-terminal domain had a large effect, with the topoIIalpha-CTD increasing activity, and the topoIIbeta-CTD decreasing activity.In vivo complementation data show that the topoIIalpha C-terminal domain is needed for growth, but the topoIIbeta isoform is able to support low levels of growth without a C-terminal domain. This may indicate that topoIIbeta has an additional localisation signal. In vitro data suggest that, while the lack of any C-terminal domain has little effect on activity, the presence of either the topoIIalpha or beta C-terminal domain can affect strand passage activity. Data indicates that the topoIIbeta-CTD may be a negative regulator. This is the first report of in vitro data with chimeric human topoIIs