Preparation and Characterisation of Nobiletin-Loaded Nanostructured Lipid Carriers

The objective of this manuscript was to investigate and optimise the potential of nanostructured lipid carriers (NLCs) as a carrier system for nobiletin (NOB), which was prepared by high-pressure homogenisation method. Additionally, this study was focused on the application of NOB-loaded NLC (NOB-NLC) in functional food. Response surface method with a three-level Box–Behnken design was validated through analysis of variance, and the robustness of the design was confirmed through the correspondence between the values measured in the experiments and the predicted ones. Properties of the prepared NOB-NLC, such as Z-average, polydispersity, entrapment efficiency, zeta potential, morphology, and crystallinity, were investigated. NOB-NLC exhibited a spherical shape with a diameter of 112.27 ± 5.33 nm, zeta potential of −35.1 ± 2.94 mV, a polydispersity index of 0.251 ± 0.058, and an EE of 81.06%  ±  6.02%. Results from X-ray diffraction and differential scanning calorimetry of NOB-NLC reviewed that the NOB crystal might be converted to an amorphous state. Fourier transform infrared spectroscopic analysis demonstrated that chemical interaction was absent between the compound and lipid mixture in NOB-NLC

Temporal correlation analysis between malaria and meteorological factors in Motuo County, Tibet

<p>Abstract</p> <p>Background</p> <p>Malaria has been endemic in Linzhi Prefecture in the Tibet Autonomous Region (TAR) over the past 20 years, especially in Motou County with a highest incidence in the country in recent years. Meteorological factors, such as rainfall, temperature and relative humidity in Motou County were unique compared to other areas in Tibet as well as other parts of China, thus the objective of this work was to analyse the temporal correlation between malaria incidence and meteorological factors in Motou County, in order to seek the particular interventions for malaria control.</p> <p>Methods</p> <p>The meteorological and malaria data during 1986-2009 in Motuo County were studied to analyse the statistical relationship between meteorological data time series and malaria incidence data series. Temporal correlation between malaria incidence and meteorological factors were analyzed using several statistical methods. Spearman correlation analysis was conducted to examine the association between monthly malaria incidence and meteorological variables. Cross-correlation analysis of monthly malaria incidence series and monthly meteorological data time series revealed the time lag(s) of meteorological factors preceding malaria at which the series showed strongest correlation. Multiplicative seasonal auto-regressive integrated moving average (SARIMA) models were used in the cross-correlation analysis with pre-whitening which remove seasonality and auto-correlation of meteorological data series. Differenced data analysis which called inter-annual analysis was carried out to find underlying relationship between malaria data series and meteorological data series.</p> <p>Results</p> <p>It has been revealed that meteorological variables, such as temperature, relative humidity and rainfall were the important environmental factors in the transmission of malaria. Spearman correlation analysis demonstrated relative humidity was greatest relative to malaria incidence and the correlation coefficient was 0.543(<it>P </it>< 0.01). Strong positive correlations were found for malaria incidence time series lagging one to three months behind rainfall (<it>r </it>> 0.4) and lagging zero to two months behind temperature and relative humidity (<it>r </it>> 0.5) by the cross-correlation. Correlations were weaker with pre-whitening than without. The cross-correlograms between malaria incidence and various meteorological variables were entirely different. It was fluctuated randomly for temperature but with trend for the other two factors, which showed positive correlated to malaria when lag was from 0 to 5 months and negative from 6 to 12 months. Besides, the inter-annual analysis showed strong correlation between differenced annual malaria incidence and differenced meteorological variables (annual average maximum temperature, annual average relative humidity and annual average rainfall). The correlations coefficients were -0.668 (<it>P </it>< 0.01), 0.451(<it>P </it>< 0.05) and 0.432(<it>P </it>< 0.05), respectively.</p> <p>Conclusion</p> <p>Meteorological variables play important environmental roles in malaria transmission in Motou County. Relative humidity was the greatest influence factors, which affected the mosquito survival directly. The relationship between malaria incidence and rainfall was complex and it was not directly and linearly. The lags of temperature and relative humidity were similar and smaller than that of rainfall. Since the lags of meteorological variables affecting malaria transmission were short, it was difficult to do accurate long-term malaria incidence prediction using meteorological variables.</p

Światowa produktywność badań w dziedzinie endokrynologii i metabolizmu — analiza bibliometryczna

  Introduction: Recently, significant contributions to the study of endocrinology and metabolism have been made. The national contribution, however, has not been reported. The aim of this study was to assess national efforts in the field of endocrinology and metabolism. Material and methods: A Web of Science search was performed using subject categories “endocrinology &amp;amp; metabolism” to identify articles published from 2010 to 2014. The total and per capita numbers of articles and citations were analysed for different countries. Results: A total of 79,394 articles were published on endocrinology and metabolism from 2010 to 2014. Most were published in North America, East Asia, and Europe. The majority (82.28%) were reported by authors in high-income countries, 17.64% were published in middle-income countries, and only 0.08% were published in low-income countries. Authors in the United States published the most articles (27.38%), followed by China (7.22%), Italy (5.70%), the United Kingdom (5.6%), and Japan (5.54%). Articles published by authors in the United States had the most citations (260,934). A positive correlation was found between the number of publications and population/gross domestic product (GDP; p &lt; 0.01). When normalised to population size, the ranking for the most publications was Denmark, Sweden, and the Netherlands; when normalised to GDP, the ranking was Denmark, Greece, and the Netherlands. Conclusions: The majority of endocrinology and metabolism articles were published by authors from high-income countries with few from low-income countries. The United States was the most productive country. However, when population size and GDP were considered, some European countries were ranked higher. (Endokrynol Pol 2015; 66 (5): 434–442)    Wstęp: Ostatnio pojawiło się wiele znaczących publikacji na temat badań z dziedziny endokrynologii i metabolizmu. Narodowy wkład na tym polu został jednak pominięty. Celem niniejszego badania była ocena krajowych badań w dziedzinie endokrynologii i metabolizmu. Materiał i metody: Wyszukiwanie za pomocą Web of Science przeprowadzono z wykorzystaniem kategorii podmiotowych „endokrynologia i metabolizm”, aby zidentyfikować artykuły opublikowane w latach 2010–2014. Analizie poddano łączną liczbę artykułów i cytowań, a także ich liczbę przypadającą na osobę w odniesieniu do różnych krajów. Wyniki: W latach 2010–2014 opublikowano łącznie 79 394 artykułów na temat endokrynologii i metabolizmu. Większość artykułów pochodziła z Ameryki Północnej, Azji Wschodniej i Europy. Większość artykułów (82,28%) napisali autorzy z krajów o wysokich dochodach, 17,64% opublikowano w krajach średnio zamożnych, a jedynie 0,08% artykułów opublikowano w krajach o niskich dochodach. Najwięcej artykułów publikowali autorzy ze Stanów Zjednoczonych (27,38%), następnie z Chin (7,22%), Włoch (5,70%), Wielkiej Brytanii (5,6%) i Japonii (5,54%). Prace publikowane przez amerykańskich autorów zawierały największą liczbę cytowań (260 934). Stwierdzono pozytywny związek między liczbą publikacji i populacją/produktem krajowym brutto (PKB; p &lt; 0,01). Po unormalizowaniu do liczebności populacji, w rankingu krajów o najwyższej liczbie publikacji znalazły się Dania, Szwecja oraz Holandia. Gdy znormalizowano wyniki pod względem PKB, w rankingu znalazły się Dania, Grecja oraz Holandia. Wnioski: Większość artykułów z dziedziny endokrynologii i metabolizmu została opublikowana przez autorów z krajów o wysokich dochodach; w krajach o niskich dochodach ukazało się niewiele artykułów. Stany Zjednoczone wykazały największą produktywność, jednak kiedy brano pod uwagę liczebność populacji i PKB, niektóre kraje europejskie zajmowały wyższą pozycję. (Endokrynol Pol 2015; 66 (5): 434–442)

The Discovery of A Luminous Broad Absorption Line Quasar at A Redshift of 7.02

Despite extensive efforts, only two quasars have been found at $z>7$ to date due to a combination of low spatial density and high contamination from more ubiquitous Galactic cool dwarfs in quasar selection. This limits our current knowledge of the super-massive black hole (SMBH) growth mechanism and reionization history. In this letter, we report the discovery of a luminous quasar at $z=7.021$, DELS J003836.10$-$152723.6 (hereafter J0038$-$1527), selected using photometric data from DESI Legacy imaging Survey (DELS), Pan-STARRS1 (PS1) imaging Survey, as well as Wide-field Infrared Survey Explore ($WISE$) mid-infrared all-sky survey. With an absolute magnitude of $M_{1450}$=$-$27.1 and bolometric luminosity of $L_{\rm Bol}$=5.6$\times$10$^{13}$ $L_\odot$, J0038$-$1527 is the most luminous quasar known at $z>7$. Deep optical to near infrared spectroscopic observations suggest that J0038-1527 hosts a 1.3 billion solar mass BH accreting at the Eddington limit, with an Eddington ratio of 1.25$\pm$0.19. The CIV broad emission line of J0038$-$1527 is blue-shifted by more than 3000 km s$^{-1}$ to the systemic redshift. More detailed investigations of the high quality spectra reveal three extremely high velocity CIV broad absorption lines (BALs) with velocity from 0.08 to 0.14 times the speed of light and total balnicity index of more than 5000 km s$^{-1}$, suggesting the presence of relativistic outflows. J0038$-$1527 is the first quasar found at the epoch of reionization (EoR) with such strong outflows and provides a unique laboratory to investigate AGN feedback on the formation and growth of the most massive galaxies in the early universe.Comment: ApJL in pres

Solar Ring Mission: Building a Panorama of the Sun and Inner-heliosphere

Solar Ring (SOR) is a proposed space science mission to monitor and study the Sun and inner heliosphere from a full 360{\deg} perspective in the ecliptic plane. It will deploy three 120{\deg}-separated spacecraft on the 1-AU orbit. The first spacecraft, S1, locates 30{\deg} upstream of the Earth, the second, S2, 90{\deg} downstream, and the third, S3, completes the configuration. This design with necessary science instruments, e.g., the Doppler-velocity and vector magnetic field imager, wide-angle coronagraph, and in-situ instruments, will allow us to establish many unprecedented capabilities: (1) provide simultaneous Doppler-velocity observations of the whole solar surface to understand the deep interior, (2) provide vector magnetograms of the whole photosphere - the inner boundary of the solar atmosphere and heliosphere, (3) provide the information of the whole lifetime evolution of solar featured structures, and (4) provide the whole view of solar transients and space weather in the inner heliosphere. With these capabilities, Solar Ring mission aims to address outstanding questions about the origin of solar cycle, the origin of solar eruptions and the origin of extreme space weather events. The successful accomplishment of the mission will construct a panorama of the Sun and inner-heliosphere, and therefore advance our understanding of the star and the space environment that holds our life.Comment: 41 pages, 6 figures, 1 table, to be published in Advances in Space Researc

Reduced arsenic clearance and increased toxicity in aquaglyceroporin-9-null mice

Expressed in liver, aquaglyceroporin-9 (AQP9) is permeated by glycerol, arsenite, and other small, neutral solutes. To evaluate a possible protective role, AQP9-null mice were evaluated for in vivo arsenic toxicity. After injection with NaAsO(2), AQP9-null mice suffer reduced survival rates (LD(50), 12 mg/kg) compared with WT mice (LD(50), 15 mg/kg). The highest tissue level of arsenic is in heart, with AQP9-null mice accumulating 10-20 times more arsenic than WT mice. Within hours after NaAsO(2) injection, AQP9-null mice sustain profound bradycardia, despite normal serum electrolytes. Increased arsenic levels are also present in liver, lung, spleen, and testis of AQP9-null mice. Arsenic levels in the feces and urine of AQP9-null mice are only approximately 10% of the WT levels, and reduced clearance of multiple arsenic species by the AQP9-null mice suggests that AQP9 is involved in the export of multiple forms of arsenic. Immunohistochemical staining of liver sections revealed that AQP9 is most abundant in basolateral membrane of hepatocytes adjacent to the sinusoids. AQP9 is not detected in heart or kidney by PCR or immunohistochemistry. We propose that AQP9 provides a route for excretion of arsenic by the liver, thereby providing partial protection of the whole animal from arsenic toxicity

Exploring Reionization-era Quasars. III. Discovery of 16 Quasars at 6.4 ≲ z ≲ 6.9 with DESI Legacy Imaging Surveys and the UKIRT Hemisphere Survey and Quasar Luminosity Function at z ∼ 6.7

This is the third paper in a series aimed at finding reionization-era quasars with the combination of DESI Legacy imaging Surveys (DELS), the Pan-STARRS1 (PS1) Survey, and near-infrared imaging surveys, such as the UKIRT Hemisphere Survey (UHS), as well as the Wide-field Infrared Survey Explorer (WISE) mid-infrared survey. In this paper, we describe the updated quasar candidate selection procedure, report the discovery of 16 quasars at 6.4 z 6.9 from an area of ~13,020 deg2, and present the quasar luminosity function (QLF) at z ~ 6.7. The measured QLF follows in the magnitude range −27.6 5 that was found by previous works continues to z > 6, at a rate significantly faster than the average decline rate between z ~ 3 and 5. We measured quasar comoving emissivity at z ~ 6.7, which indicates that high-redshift quasars are highly unlikely to make a significant contribution to hydrogen reionization. The broad absorption line quasar fraction at z 6.5 is measured to be 22%. In addition, we also report the discovery of six additional quasars at z ~ 6 in the Appendix

Expert Consensus on Microtransplant for Acute Myeloid Leukemia in Elderly Patients -Report From the International Microtransplant Interest Group

Recent studies have shown that microtransplant (MST) could improve outcome of patients with elderly acute myeloid leukemia (EAML). To further standardize the MST therapy and improve outcomes in EAML patients, based on analysis of the literature on MST, especially MST with EAML from January 1st, 2011 to November 30th, 2022, the International Microtransplant Interest Group provides recommendations and considerations for MST in the treatment of EAML. Four major issues related to MST for treating EAML were addressed: therapeutic principle of MST (1), candidates for MST (2), induction chemotherapy regimens (3), and post-remission therapy based on MST (4). Others included donor screening, infusion of donor cells, laboratory examinations, and complications of treatment

Bioactivity-Guided Identification of Anti-Adipogenic Isothiocyanates in the Moringa (Moringa oleifera) Seed and Investigation of the Structure-Activity Relationship

Due to the side effects of obesity medications, many studies have focused on the natural products used in the daily diet to control weight. Moringa seed pods and leaves are widely used as vegetables or diet supplements due to the high nutrition value. However, no bioactivity-guided anti-adipogenic study was previously conducted. Therefore, a preadipocyte cell line was adopted as the bioactivity assay to identify the anti-adipogenic compounds in the peeled Moringa seed. Two known sulphur-containing compounds (1 and 2) were isolated and identified. Compound 2, 4-(&alpha;-l-rhamnosyloxy) benzyl isothiocyanate, showed a great anti-adipogneic effect with an IC50 value of 9.2 &mu;g/mL. The isothiocyanate (ITC) group in compound 2 could be responsible for the inhibitory activity. In addition, a series of compounds with the ITC group were used to further investigate the structure-activity relationship, indicating foods containing ITC derivatives have the potential of being used to control weight