Textbooks History of art culture for art and handicraft of secondary vocational schools: didactic analysis of texts

The thesis "School Textbooks of History of Visual Culture for Secondary Art Schools (Didactic analysis of texts)" is focused on the area of visual culture textbooks for professional secondary art education. The secondary art education in the territory of Czech Republic has a long tradition since the middle of 19th century and art history tuition realized in the school subject History of Visual Culture presents a considerable part of professional training of future artists, artisans and craftsmen. The main aim of the school subject is providing students with a complex and systematically structured knowledge of visual culture evolution, art trends and tendencies from the very beginning until the present days. This knowledge will be essential theoretical background for students' professional activities in the field of visual creation. Powered by TCPDF (www.tcpdf.org

Size-Dependent Immunochromatographic Assay with Quantum Dot Nanobeads for Sensitive and Quantitative Detection of Ochratoxin A in Corn

Fluorescent microspheres are a novel luminescent nanomaterial proposed as an alternative probe to improve the detection sensitivity of competitive immunochromatographic assay (ICA). Quantum dot nanobeads (QBs) possess strong luminescence and resistance to matrix interference. Theoretically, large-sized QBs exhibit stronger luminescent intensity than small-sized QBs and are beneficial to ICA sensitivity. However, oversized QBs may reduce the sensitivity of competitive ICA. Thus, the relationship between the size and luminescent intensity of QBs and the competitive ICA sensitivity must be elucidated. In this study, QBs of different sizes (58, 124, 255, 365, and 598 nm) were synthesized. Ochratoxin A (OTA) was selected as the model analyte for competitive ICA. The effects of QB size on the detection performance of competitive ICA were then evaluated. The cutoff limit of QB-ICA for naked eye detection was used for qualitative analysis, and the half-maximal inhibitory concentration (IC₅₀) and LOD were employed for quantitative analysis. Results indicated that 124 nm QBs used as labeling probes for competitive ICA showed the optimal detection performance and the lowest cutoff value of 5 ng/mL for qualitative detection and IC₅₀ (0.39 ng/mL) for quantitative detection. Similar to commercial ELISA, QB₁₂₄-ICA displayed good accuracy, specificity, reproducibility, and practicability. In summary, 124 nm QBs can be used as a new labeling probe for competitive ICA

Additional file 2: Figure S3. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

Characterization of the cellular infiltrate and histomorphologic grading of LSG. (A) Shown are representative examples of grade 1 (G1; <50 periductal lymphocytes); (B,C) grade 2 (G2; >50 periductal lymphocytes): nonsegregated and segregated aggregates (NS-G2 and S-G2, respectively); (D,F) grade 3 (G3; >50 periductal lymphocytes, with GC-like structures). (TIF 58923 kb

Additional file 7: Figure S2. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

Immunohistochemistry for TLR9 and CXCR4 in LSG of pSS and healthy controls. (A, E) Infiltrating lymphocytes in LSG of pSS positively stained for TLR9 (200× and 400× magnification); (B, F) glandular epithelial cells from control were negative (200× and 400× magnification); (C, G) infiltrating lymphocytes and adjacent glandular epithelial cells in LSG of pSS were positively stained for CXCR4 (200× and 400× magnification). (D, H) Glandular epithelial cells from control were negative (200× and 400× magnification). (TIF 28419 kb

Additional file 1: Table S1. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

Primer sequences used in validation of lncRNAs. (DOCX 14 kb

Additional file 6: Figure S1. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

Immunohistochemistry for CD19 and ICAM1 in LSG of pSS and healthy control. (A, E) Infiltrating lymphocytes in LSG of pSS were positively staining for CD19 (200× and 400× magnification); (B, F) glandular epithelial cells from control were negative (200× and 400× magnification); (C, G) infiltrating lymphocytes and adjacent glandular epithelial cells in LSG of pSS were positively staining for ICAM1 (200× and 400× magnification). (D, H) Glandular epithelial cells from control were negative (200× and 400× magnification). (TIF 28780 kb

Additional file 3: Figure S4. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

EBER expression in LSG of pSS patients. (A) Significant number of EBER+ cells in representative ectopic lymphoid structure-positive LSG tissue. (B) Double staining for CD3 and CD20 in the same section of LSG tissue. (C) Staining for CD21 in the same section of LSG tissue. (TIF 53011 kb