Deciduous Trees and the Application of Universal DNA Barcodes: A Case Study on the Circumpolar Fraxinus

The utility of DNA barcoding for identifying representative specimens of the circumpolar tree genus Fraxinus (56 species) was investigated. We examined the genetic variability of several loci suggested in chloroplast DNA barcode protocols such as matK, rpoB, rpoC1 and trnH-psbA in a large worldwide sample of Fraxinus species. The chloroplast intergenic spacer rpl32-trnL was further assessed in search for a potentially variable and useful locus. The results of the study suggest that the proposed cpDNA loci, alone or in combination, cannot fully discriminate among species because of the generally low rates of substitution in the chloroplast genome of Fraxinus. The intergenic spacer trnH-psbA was the best performing locus, but genetic distance-based discrimination was moderately successful and only resulted in the separation of the samples at the subgenus level. Use of the BLAST approach was better than the neighbor-joining tree reconstruction method with pairwise Kimura's two-parameter rates of substitution, but allowed for the correct identification of only less than half of the species sampled. Such rates are substantially lower than the success rate required for a standardised barcoding approach. Consequently, the current cpDNA barcodes are inadequate to fully discriminate Fraxinus species. Given that a low rate of substitution is common among the plastid genomes of trees, the use of the plant cpDNA “universal” barcode may not be suitable for the safe identification of tree species below a generic or sectional level. Supplementary barcoding loci of the nuclear genome and alternative solutions are proposed and discussed

Ätiologie und Therapie der Streptokokken-Infectionen

Layoutgetreues Digitalisat der Ausg.: Berlin ; Wien, 1899 Standort: Fachgebiet für Geschichte der Medizin (192) Signatur: 226 Provenienz: Behring, Emil vo

Aetiologie und Therapie der Staphylokokken-Infectionen

Layoutgetreues Digitalisat der Ausg.: Berlin ; Wien, 1900 Standort: Fachgebiet für Geschichte der Medizin (192) Signatur: 226 Provenienz: Behring, Emil vo

Conclusionum maxime controversarum ex utroque iure civili & canonico depromptarum decades duae

quas ... pro summis in utroque iure honoribus, privilegiis ac immunitatibus legitimè consequendis publicae censurae submittit Ludovicus Lingelshemius Heidelbergensis ad diem ... iun, an. 1643 horis locoque solitisDatum auf der Titels. hs. erg.: ad diem 9 Iun.Enthält 20 ThesenDiss. iur. Basel, 164