Genetic variations among ecologically diverse species of Anurans at the level of genus based on ISSR marker

Anurans (frogs and toads) collected from South Kanara, Udupi and North Kanara districts, Karnataka viz: Euphlyctis cyanophlyctis (an aquatic), Fejervarya rufescens (a burrowing), Duttaphrynus melanostictus (a terrestrial), Fejervarya caperata (a semi aquatic), Ramanella montana (a semi arboreal) and Polypedates maculatus (an arboreal) belonging to five genera placed under four families were compared using Inter Simple Sequence Repeat (ISSR) marker to analyze genetic variability and relationship between the investigated taxa. ISSR profile obtained exhibited 100% presence of polymorphic bands.The ISSR bands could also be categorized as high (H), moderate (M) and light (L) based on the intensity of amplification. Dendrograms were generated using similarity coefficient and relationship coefficient values based on the count of only high or high and moderate or high, moderate and light bands in each case. Analysis of the data suggests that the genetic relatedness between the taxa as exhibited in the generated dendrograms varies based on the type of amplified bands considered. The clustering pattern obtained in the case of high and moderate bands was more close to the conventional taxonomical pattern of grouping based on thephenotypic characters. The specific unique bands present in each case may be of importance as molecular markers. Observed genetic variability (presence/absence, total number, intensity of amplification and size of polymorphic bands) among taxa probably provide factors contributing partially to diverse adaptive fitness supporting varied ecological demands. However, an intensive study incorporating more representive species from each of the habitat is needed to pinpoint the importance of the unique bands as the genus/species/ecological molecular markers

Conversion of fructose into 5-hydroxy methyl furfural over Mesoporous-ZrO2-phosphomolybdic acid nanocomposite catalysts

A series of phosphomolybdic acid (MPA) with varying ts contant incorporated into the mesoporous zirconia (ZMPA) catalysts were prepared by surfactant-assisted sol-gel copolymerization technique. These catalysts were evaluated for selective dehydration of fructose to 5-hydroxymethyl furfural (HMF). The catalysts were characterized by X-ray diffraction, nitrogen physisorption, Fourer-Transform infrared, temperature programmed disorption, pyridine adsorbed FT-infrared and transmssion electron microscopy. The characterization results suggest that these catalysts possess ordered mesoporous structure with Keggin heteropoly molybdate. More over the incorporation of phosphomolybdic acid into mesoporous zirconia resulted in generaton of more number of strong acidic sites. These catalysts exhibited about 80 % HMF yield with in 30 min of reaction time. The existence of relatively strong interaction between the MPA keggin units with ZrO2 mesoporous structure played crucial role in fabricating the stable heterogeneous catalyst. This catalyst is reusable with constant activity

Association Analysis of F 2 Generation in Rice (Oryza sativa. L.)

ABSTRAC

Association Analysis of F 2 Generation in Rice (Oryza sativa. L.)

ABSTRAC

Ternary Complexes of Copper(II) with Some Amino Acids & 2-Substituted Benzimidazoles

875-87

Enamine derivatives of 1,2,3,4-tetrahydro-7,8-dimethoxy-1-<i>p</i>-tosyl-1-benzazepin-5-one as potential antiinflammatory agents^†

1086-10871,2 ,3 ,4-Tetrahydro-7 ,8-dimethoxy-5-pyrrolidino/piperidino/morpholino-1-p-tosyl-1-benzazepines 2-4 have been obtained in excellent yields from tetrahydrodimethoxy-p-tosyl benzazepine 1 with secondary amines in refluxing benzene. Compounds 2-4 exhibit significant antiinflammatory activity