Escherichia coli O157:H7 reduction in hamburgers with regard to premature browning of minced beef, colour score and method for determining doneness

This study investigated the effect of premature browning (PMB) on the survival of Escherichia coli O157:H7 in beef hamburgers after cooking with respect to interior colour of the hamburger and recommendations to cook hamburgers to a core temperature of 71 degrees C. Assessment of doneness by visual inspection or measurement of internal temperature was compared in terms of survival and the increased relative risk of illness due to PMB was estimated. At the last consume-by-day, hamburgers made from minced meat packaged in 80/20 O-2/CO2 (MAP hamburger) and from meat minced at retail packaged in atmospheric condition (control hamburger) were inoculated with a gfp-tagged strain of E. coli O157:H7 (E. coli O157:H7gfp+). Hamburgers were cooked for different times during assessment of the core temperature every 30 s and cut in halves after cooking. Doneness was evaluated based on visual judgement of the internal colour using a score chart (C-score) from 'uncooked' (score 1) to 'tan with no evidence of pink' (score 5). An alternative five point score chart (TCC-score) including texture of the meat, clarity of meat juice and internal colour was also developed. Enumeration of viable E. coli O157:H7gfp+ in cooked hamburgers was based on fluorescent colonies recovered from plates. Results showed that MAP hamburgers developed PMB when compared with controls (P = 0.0003) and that the shortest cooking time for the highest C-score was 6 and 11 min for MAP and control hamburgers, respectively. The mean temperature in the MAP hamburger was then 60.3 degrees C. The TCC-score reduced the difference between MAP and control hamburgers. It was also shown that the survival of E. coli O157:H7gfp+ was highest in MAP hamburgers. The predicted absolute risks for illness were highest for MAP hamburgers for all C-scores and the relative risk associated with PMB increased with doneness. For a C-score of 4 (slightly pink) the predicted relative risk for illness was 300 times higher for MAP hamburger than for controls. A variable pathogen reduction was observed when cooking hamburgers to temperatures of 70-76 degrees C (the 5th and 95th percentile range was around 33 log CFU). The lower reductions, at the 5th percentile, may, depending on initial contamination levels, not be enough to ensure sufficient and safe inactivation of E. coli O157:H7. Efforts to inform consumers about PMB in minced meat packaged in high oxygen packages (>= 60% O-2) are needed with the aim to make consumers use thermometers correctly or at least not determine doneness based only on meat colour

Preliminary results on SiO v=3 J=1-0 maser emission from AGB stars

We present the results of SiO maser observations at 43GHz toward two AGB stars using the VLBA. Our preliminary results on the relative positions of the different J=1-0 SiO masers (v=1,2 and 3) indicate that the current ideas on SiO maser pumping could be wrong at some fundamental level. A deep revision of the SiO pumping models could be necessary.Comment: poster, 2 pages, 2 figures, Proc. IAU Symp. 287 "Cosmic Masers: from OH to H0", R.S. Booth, E.M.L. Humphreys and W.H.T. Vlemmings, ed

SiO masers from AGB stars in the vibrationally excited v=1,v=2, and v=3 states

The v=1 and v=2 J=1-0 (43 GHz), and v=1 J=2-1 (86 GHz) SiO masers are intense in AGB stars and have been mapped using VLBI showing ring-like distributions. Those of the v=1, v=2 J=1-0 masers are similar, but the spots are rarely coincident, while the v=1 J=2-1 maser arises from a well separated region farther out. These relative locations can be explained by models tools that include the overlap of two IR lines of SiO and H2O. The v=3 J=1-0 line is not directly affected by any line overlap and its spot structure and position, relative to the other lines, is a good test to the standard pumping models. We present single-dish and simultaneous VLBI observations of the v=1, v=2, and v=3 J=1-0 maser transitions of 28SiO in several AGB stars. The spatial distribution of the SiO maser emission in the v=3 J=1-0 transition from AGB stars is systematically composed of a series of spots that occupy a ring-like structure. The overall ring structure is extremely similar to that found in the other 43 GHz transitions and is very different from the structure of the v=1 J=2-1 maser. The positions of the individual spots of the different 43 GHz lines are, however, very rarely coincident, which in general is separated by about 0.3 AU (between 1 and 5 mas). These results are very difficult to reconcile with standard pumping models, which predict that the masers of rotational transitions within a given vibrational state require very similar excitation conditions, while the transitions of different vibrational states should appear in different positions. However, models including line overlap tend to predict v=1, v=2, v=3 J=1-0 population inversion to occur under very similar conditions, while the requirements for v=1 J=2-1 appear clearly different, and are compatible with the observational results.Comment: 9 pages, 4 figures accepted by A&

Electrostatic Turbulence and Debye-scale Structures in Collisionless Shocks

We present analysis of more than 100 large-amplitude bipolar electrostatic structures in a quasi-perpendicular supercritical Earth's bow shock crossing, measured by the Magnetospheric Multiscale spacecraft. The occurrence of the bipolar structures is shown to be tightly correlated with magnetic field gradients in the shock transition region. The bipolar structures have negative electrostatic potentials and spatial scales of a few Debye lengths. The bipolar structures propagate highly oblique to the shock normal with velocities (in the plasma rest frame) of the order of the ion-acoustic velocity. We argue that the bipolar structures are ion phase space holes produced by the two-stream instability between incoming and reflected ions. This is the first identification of the ion two-stream instability in collisionless shocks

SiO Maser Forest at the Galactic Center

A moderately deep survey of stellar maser sources toward the Galactic center has been made in the SiO J=1-0 v=1 and v=2 transitions (~ 43 GHz) with the Nobeyama 45-m telescope of a beam size of about 40". At the Galactic center (Sgr A*), 7 (4 new) SiO maser sources were detected in one beam. In order to investigate the spatial distribution of SiO maser sources, we have also observed the offset positions by +- 40" in Galactic longitude from the Galactic center. In total, 7 (4 new) SiO maser sources were detected at the offset positions. Taking into account the shorter integration time at the offset positions, we find that the surface number density of SiO maser sources is nearly constant at the Galactic center. The number density of SiO maser sources is found to be an order of magnitude higher than the density of OH 1612 MHz objects. A radial-velocity gradient in Galactic longitude was not detected. These facts indicate that the SiO maser sources seen toward the Galactic center are mostly associated with the stellar population of a Galactic stellar nuclear disk of more than a few arc minute radius.Comment: 15 pages, 1 figure, Astrophysical Journal (Letters) in pres

Prevalence of abnormal findings when adopting new national and international Global Lung Function Initiative reference values for spirometry in the Finnish general population

Peer reviewe

The GOAL study: a prospective examination of the impact of factor V Leiden and ABO(H) blood groups on haemorrhagic and thrombotic pregnancy outcomes

Factor V Leiden (FVL) and ABO(H) blood groups are the common influences on haemostasis and retrospective studies have linked FVL with pregnancy complications. However, only one sizeable prospective examination has taken place. As a result, neither the impact of FVL in unselected subjects, any interaction with ABO(H) in pregnancy, nor the utility of screening for FVL is defined. A prospective study of 4250 unselected pregnancies was carried out. A venous thromboembolism (VTE) rate of 1·23/1000 was observed, but no significant association between FVL and pre-eclampsia, intra-uterine growth restriction or pregnancy loss was seen. No influence of FVL and/or ABO(H) on ante-natal bleeding or intra-partum or postpartum haemorrhage was observed. However, FVL was associated with birth-weights >90th centile [odds ratio (OR) 1·81; 95% confidence interval (CI<sub>95</sub>) 1·04–3·31] and neonatal death (OR 14·79; CI<sub>95</sub> 2·71–80·74). No association with ABO(H) alone, or any interaction between ABO(H) and FVL was observed. We neither confirmed the protective effect of FVL on pregnancy-related blood loss reported in previous smaller studies, nor did we find the increased risk of some vascular complications reported in retrospective studies

G359.87+0.18: An FR II Radio Galaxy 15 Arcminutes from Sgr A*. Implications for the Scattering Region in the Galactic Center

G359.87+0.18 is an enigmatic object located 15' from Sgr A*. It has been variously classified as an extragalactic source, Galactic jet source, and young supernova remnant. We present new observations of G359.87+0.18 between 0.33 and 15 GHz and use these to argue that this source is an Faranoff-Riley II radio galaxy. We are able to place a crude limit on its redshift of z > 0.1. The source has a spectral index \alpha < -1 (S \propto \nu^\alpha), suggestive of a radio galaxy with a redshift z >~ 2. The scattering diameters of Sgr A* and several nearby OH masers (~ 1" at 1 GHz) indicate that a region of enhanced scattering is along the line of sight to the Galactic center. If the region covers the Galactic center uniformly, the implied diameter for a background source is at least 600" at 0.33 GHz, in contrast with the observed 20" diameter of G359.87+0.18. Using the scattering diameter of a nearby OH maser OH 359.762+0.120 and the widths of two, nearby, non-thermal threads, G0.08+0.15 and G359.79+0.17, we show that a uniform scattering region should cover G359.87+0.18. We therefore conclude that the Galactic center scattering region is inhomogeneous on a scale of 5' (~ 10 pc at a distance of 8.5 kpc). This scale is comparable to the size scale of molecular clouds in the Galactic center. The close agreement between these two lengths scales is an indication that the scattering region is linked intimately to the Galactic center molecular clouds.Comment: Accepted for publication in the ApJ, vol. 515, LaTeX2e manuscript using aaspp4 macro, 19 pages, 8 figures in 11 PostScript file

The proper motion of the Arches cluster with Keck Laser-Guide Star Adaptive Optics

We present the first measurement of the proper motion of the young, compact Arches cluster near the Galactic center from near-infrared adaptive optics (AO) data taken with the recently commissioned laser-guide star (LGS) at the Keck 10-m telescope. The excellent astrometric accuracy achieved with LGS-AO provides the basis for a detailed comparison with VLT/NAOS-CONICA data taken 4.3 years earlier. Over the 4.3 year baseline, a spatial displacement of the Arches cluster with respect to the field population is measured to be 24.0 +/- 2.2 mas, corresponding to a proper motion of 5.6 +/- 0.5 mas/yr or 212 +/- 29 km/s at a distance of 8 kpc. In combination with the known line-of-sight velocity of the cluster, we derive a 3D space motion of 232 +/- 30 km/s of the Arches relative to the field. The large proper motion of the Arches cannot be explained with any of the closed orbital families observed in gas clouds in the bar potential of the inner Galaxy, but would be consistent with the Arches being on a transitional trajectory from x1 to x2 orbits. We investigate a cloud-cloud collision as the possible origin for the Arches cluster. The integration of the cluster orbit in the potential of the inner Galaxy suggests that the cluster passes within 10 pc of the supermassive black hole only if its true GC distance is very close to its projected distance. A contribution of young stars from the Arches cluster to the young stellar population in the inner few parsecs of the GC thus appears increasingly unlikely. The measurement of the 3D velocity and orbital analysis provides the first observational evidence that Arches-like clusters do not spiral into the GC. This confirms that no progenitor clusters to the nuclear cluster are observed at the present epoch.Comment: 22 pdflatex pages including 12 figures, reviewed version accepted by Ap

The slope of the black-hole mass versus velocity dispersion correlation

Observations of nearby galaxies reveal a strong correlation between the mass of the central dark object M and the velocity dispersion sigma of the host galaxy, of the form log(M/M_sun) = a + b*log(sigma/sigma_0); however, published estimates of the slope b span a wide range (3.75 to 5.3). Merritt & Ferrarese have argued that low slopes (<4) arise because of neglect of random measurement errors in the dispersions and an incorrect choice for the dispersion of the Milky Way Galaxy. We show that these explanations account for at most a small part of the slope range. Instead, the range of slopes arises mostly because of systematic differences in the velocity dispersions used by different groups for the same galaxies. The origin of these differences remains unclear, but we suggest that one significant component of the difference results from Ferrarese & Merritt's extrapolation of central velocity dispersions to r_e/8 (r_e is the effective radius) using an empirical formula. Another component may arise from dispersion-dependent systematic errors in the measurements. A new determination of the slope using 31 galaxies yields b=4.02 +/- 0.32, a=8.13 +/- 0.06, for sigma_0=200 km/s. The M-sigma relation has an intrinsic dispersion in log M that is no larger than 0.3 dex. In an Appendix, we present a simple model for the velocity-dispersion profile of the Galactic bulge.Comment: 37 pages, 9 figure