5,980 research outputs found
User documentation for the MSK and OMS intelligent tutoring systems
This user's guide describes how to use the Intelligent Tutoring Systems for the Manual Select Keyboard (MSK) and the Orbital Maneuvering System (OMS) and how to use the C code that runs the mockup version of the MSK
Embedded Ribbons of Graphene Allotropes: An Extended Defect Perspective
Four fundamental dimer manipulations can be used to produce a variety of
localized and extended defect structures in graphene. Two-dimensional templates
result in graphene allotropes, here viewed as extended defects, which can
exhibit either metallic or semiconducting electrical character. \emph{Embedded
allotropic ribbons}--i.e. thin swaths of the new allotropes--can also be
created within graphene. We examine these ribbons and find that they maintain
the electrical character of their parent allotrope even when only a few atoms
in width. Such extended defects may facilitate the construction of monolithic
electronic circuitry.Comment: 24 pages, 21 figure
Building Local Capacity to Respond to Environmental Change: Lessons and Case Studies from New York State
CaRDI Reports Issue 1
A simultaneous search for prompt radio emission associated with the short GRB 170112A using the all-sky imaging capability of the OVRO-LWA
We have conducted the most sensitive low frequency (below 100 MHz) search to
date for prompt, low-frequency radio emission associated with short-duration
gamma-ray bursts (GRBs), using the Owens Valley Radio Observatory Long
Wavelength Array (OVRO-LWA). The OVRO-LWA's nearly full-hemisphere
field-of-view (, square degrees) allows us to search for
low-frequency (sub- MHz) counterparts for a large sample of the subset of
GRB events for which prompt radio emission has been predicted. Following the
detection of short GRB 170112A by Swift, we used all-sky OVRO-LWA images
spanning one hour prior to and two hours following the GRB event to search for
a transient source coincident with the position of GRB 170112A. We detect no
transient source, with our most constraining flux density limit of
for frequencies spanning . We
place constraints on a number of models predicting prompt, low-frequency radio
emission accompanying short GRBs and their potential binary neutron star merger
progenitors, and place an upper limit of on the fraction of energy released in the prompt radio
emission. These observations serve as a pilot effort for a program targeting a
wider sample of both short and long GRBs with the OVRO-LWA, including bursts
with confirmed redshift measurements which are critical to placing the most
constraining limits on prompt radio emission models, as well as a program for
the follow-up of gravitational wave compact binary coalescence events detected
by advanced LIGO and Virgo.Comment: 14 pages, 5 figures, ApJ submitte
Multiple Gene Variants Linked to Alzheimer\u27s-Type Clinical Dementia via GWAS are Also Associated with Non-Alzheimer\u27s Neuropathologic Entities
The classic pathologic hallmarks of Alzheimer’s disease (AD) are amyloid plaques and neurofibrillary tangles (AD neuropathologic changes, or ADNC). However, brains from individuals clinically diagnosed with “AD-type” (amnestic) dementia usually harbor heterogeneous neuropathologies in addition to, or other than, ADNC. We hypothesized that some AD-type dementia associated genetic single nucleotide variants (SNVs) identified from large genomewide association studies (GWAS) were associated with non-ADNC neuropathologies. To test this hypothesis, we analyzed data from multiple studies with available genotype and neuropathologic phenotype information. Clinical AD/dementia risk alleles of interest were derived from the very large GWAS by Bellenguez et al. (2022) who reported 83 clinical AD/dementia-linked SNVs in addition to the APOE risk alleles. To query the pathologic phenotypes associated with variation of those SNVs, National Alzheimer’s disease Coordinating Center (NACC) neuropathologic data were linked to AD Sequencing Project (ADSP) and AD Genomics Consortium (ADGC) data. Separate data were obtained from the harmonized Religious Orders Study and the Rush Memory and Aging Project (ROSMAP). A total of 4811 European participants had at least ADNC neuropathology data and also genotype data available; data were meta-analyzed across cohorts. As expected, a subset of dementia-associated SNVs were associated with ADNC risk in Europeans—e.g., BIN1, PICALM, CR1, MME, and COX7C. Other gene variants linked to (clinical) AD dementia were associated with non-ADNC pathologies. For example, the associations of GRN and TMEM106B SNVs with limbic-predominant age-related TDP-43 neuropathologic changes (LATE-NC) were replicated. In addition, SNVs in TNIP1 and WNT3 previously reported as ADrelated were instead associated with hippocampal sclerosis pathology. Some genotype/neuropathology association trends were not statistically significant at P \u3c 0.05 after correcting for multiple testing, but were intriguing. For example, variants in SORL1 and TPCN1 showed trends for association with LATE-NC whereas Lewy body pathology trended toward association with USP6NL and BIN1 gene variants. A smaller cohort of non-European subjects (n = 273, approximately one-half of whom were African-Americans) provided the basis for additional exploratory analyses. Overall, these findings were consistent with the hypothesis that some genetic variants linked to AD dementia risk exert their affect by influencing non-ADNC neuropathologies
Gentamicin induces LAMB3 nonsense mutation readthrough and restores functional laminin 332 in junctional epidermolysis bullosa
Herlitz junctional epidermolysis bullosa (H-JEB) is an incurable, devastating, and mostly fatal inherited skin disease for which there is only supportive care. H-JEB is caused by loss-of-function mutations in LAMA3, LAMB3, or LAMC2, leading to complete loss of laminin 332, the major component of anchoring filaments, which mediate epidermal-dermal adherence. LAMB3 (laminin \u3b23) mutations account for 80% of patients with H-JEB, and 3c95% of H-JEB\u2013associated LAMB3 mutations are nonsense mutations leading to premature termination codons (PTCs). In this study, we evaluated the ability of gentamicin to induce PTC readthrough in H-JEB laminin \u3b23-null keratinocytes transfected with expression vectors encoding eight different LAMB3 nonsense mutations. We found that gentamicin induced PTC readthrough in all eight nonsense mutations tested. We next used lentiviral vectors to generate stably transduced H-JEB cells with the R635X and C290X nonsense mutations. Incubation of these cell lines with various concentrations of gentamicin resulted in the synthesis and secretion of full-length laminin \u3b23 in a dose-dependent and sustained manner. Importantly, the gentamicin-induced laminin \u3b23 led to the restoration of laminin 332 assembly, secretion, and deposition within the dermal/epidermal junction, as well as proper polarization of \u3b16\u3b24 integrin in basal keratinocytes, as assessed by immunoblot analysis, immunofluorescent microscopy, and an in vitro 3D skin equivalent model. Finally, newly restored laminin 332 corrected the abnormal cellular phenotype of H-JEB cells by reversing abnormal cell morphology, poor growth potential, poor cell-substratum adhesion, and hypermotility. Therefore, gentamicin may offer a therapy for H-JEB and other inherited skin diseases caused by PTC mutations
The Radio Sky at Meter Wavelengths: m-Mode Analysis Imaging with the Owens Valley Long Wavelength Array
A host of new low-frequency radio telescopes seek to measure the 21-cm
transition of neutral hydrogen from the early universe. These telescopes have
the potential to directly probe star and galaxy formation at redshifts , but are limited by the dynamic range they can achieve
against foreground sources of low-frequency radio emission. Consequently, there
is a growing demand for modern, high-fidelity maps of the sky at frequencies
below 200 MHz for use in foreground modeling and removal. We describe a new
widefield imaging technique for drift-scanning interferometers,
Tikhonov-regularized -mode analysis imaging. This technique constructs
images of the entire sky in a single synthesis imaging step with exact
treatment of widefield effects. We describe how the CLEAN algorithm can be
adapted to deconvolve maps generated by -mode analysis imaging. We
demonstrate Tikhonov-regularized -mode analysis imaging using the Owens
Valley Long Wavelength Array (OVRO-LWA) by generating 8 new maps of the sky
north of with 15 arcmin angular resolution, at frequencies
evenly spaced between 36.528 MHz and 73.152 MHz, and 800 mJy/beam thermal
noise. These maps are a 10-fold improvement in angular resolution over existing
full-sky maps at comparable frequencies, which have angular resolutions . Each map is constructed exclusively from interferometric observations
and does not represent the globally averaged sky brightness. Future
improvements will incorporate total power radiometry, improved thermal noise,
and improved angular resolution -- due to the planned expansion of the OVRO-LWA
to 2.6 km baselines. These maps serve as a first step on the path to the use of
more sophisticated foreground filters in 21-cm cosmology incorporating the
measured angular and frequency structure of all foreground contaminants.Comment: 27 pages, 18 figure
- …