Exploration on the Cultivation of Iron Man Spirit of Petroleum College Students in the New Era—Take H School as an Example

As the main position for cultivating the iron man spirit of petroleum college students, petroleum colleges and universities, While effectively helping college students in petroleum universities to create their beautiful life and brilliant life, educating them with the spirit of Iron Man is conducive to cultivating their will to work hard in the process of inheriting the spirit of Iron Man, and possessing the character of selfless dedication in their own growth and development. Taking the students in H school as the research object, the current situation of cultivating the iron man spirit of college students was investigated and analyzed mainly by questionnaire. The main problems are that the scientific and effective teaching methods for cultivating teachers need to be improved, the resources for cultivating iron man spirit practice in petroleum universities are not fully utilized, and students lack the effective combination of iron man spirit and their own reality. In view of the existing problems, we can further improve the quality of cultivating iron man spirit in petroleum universities in the new era by improving the level of cultivating teachers, improving the cultivation approach, optimizing the cultivation environment and paying attention to the cultivation subject

Identifkasi Bahaya, Penilaian, dan Pengendalian Risiko Proses Pengangkutan Batu Bara di PT Alam Karya Gemilang Kabupaten Kutai Kartanegara Provinsi Kalimantan Timur

Transportation of coal production results is an activity of moving coal from the front loading area to the storage area for further transportation to the barge. The process of transporting coal is an important activity in mining activities that have a very high occupational health and safety risk. The purpose of conducting this research is to improve hazard, risk assessment, and control in the coal hauling process carried out by PT Alam Karya Gemilang. The type of research method used is descriptive qualitative research. This research method is used to describe descriptively the assessment and control of occupational safety and health risks using the hazard identification risk assessment risk control (HIRARC) method at PT Alam Karya Gemilang. Data collection was carried out by direct field observation and interviews with unit operators, field supervisors, and safety personnel in the field. The results of the study contained 16 hazards which included: slippery front area, narrow front area, loading coal during the day and night, dusty roads, foggy roads, damaged units, blind spot areas, exhaust emissions, interaction with heavy equipment units, slippery roads, and bumpy roads. And of the 21 hazards there are 24 identified risks, namely 4 hazards with low risk, 8 hazards with moderate risk, and 13 hazards with high risk. Controls carried out are administrative controls and engineering techniques

De novo characterization of Larix gmelinii (Rupr.) Rupr. transcriptome and analysis of its gene expression induced by jasmonates

BACKGROUND: Larix gmelinii is a dominant tree species in China’s boreal forests and plays an important role in the coniferous ecosystem. It is also one of the most economically important tree species in the Chinese timber industry due to excellent water resistance and anti-corrosion of its wood products. Unfortunately, in Northeast China, L. gmelinii often suffers from serious attacks by diseases and insects. The application of exogenous volatile semiochemicals may induce and enhance its resistance against insect or disease attacks; however, little is known regarding the genes and molecular mechanisms related to induced resistance. RESULTS: We performed de novo sequencing and assembly of the L. gmelinii transcriptome using a short read sequencing technology (Illumina). Chemical defenses of L. gmelinii seedlings were induced with jasmonic acid (JA) or methyl jasmonate (MeJA) for 6 hours. Transcriptomes were compared between seedlings induced by JA, MeJA and untreated controls using a tag-based digital gene expression profiling system. In a single run, 25,977,782 short reads were produced and 51,157 unigenes were obtained with a mean length of 517 nt. We sequenced 3 digital gene expression libraries and generated between 3.5 and 5.9 million raw tags, and obtained 52,040 reliable reference genes after removing redundancy. The expression of disease/insect-resistance genes (e.g., phenylalanine ammonialyase, coumarate 3-hydroxylase, lipoxygenase, allene oxide synthase and allene oxide cyclase) was up-regulated. The expression profiles of some abundant genes under different elicitor treatment were studied by using real-time qRT-PCR. The results showed that the expression levels of disease/insect-resistance genes in the seedling samples induced by JA and MeJA were higher than those in the control group. The seedlings induced with MeJA elicited the strongest increases in disease/insect-resistance genes. CONCLUSIONS: Both JA and MeJA induced seedlings of L. gmelinii showed significantly increased expression of disease/insect-resistance genes. MeJA seemed to have a stronger induction effect than JA on expression of disease/insect-resistance related genes. This study provides sequence resources for L. gmelinii research and will help us to better understand the functions of disease/insect-resistance genes and the molecular mechanisms of secondary metabolisms in L. gmelinii

Effects of Nanoparticles on Double-Stranded RNA Stability in Moth Hemolymph

RNA interference (RNAi) is an immune response in which double-stranded RNA (dsRNA) suppresses a target gene. By designing dsRNA to target genes that are necessary for life, dsRNA can potentially be used as an insecticide. RNAi-based insecticides are badly needed because they are more specific than conventional pesticides and because many insects have developed resistance to pesticides. Unfortunately, some insects produce enzymes that degrade dsRNA and prevent the RNAi response (Cooperet al., 2018). Therefore, RNAi-based insecticides currently cannot be used to control all insects. Here we investigate dsRNA stability when incubated in hemolymph ex vivoto determine if degradation of dsRNA is contributing to the inadequate RNAi response exhibited by lepidopterans, such as the European corn borer (ECB, Ostrinia nubilalis). Our findings indicate that dsRNA is significantly degraded in ECB hemolymph, but encapsulation of dsRNA in chitosan-based nanoparticles (CB-NPs) enhances stability. These findings provide insight into RNAi efficiency limitations in insects, and may provide a method to enhance RNAi efficiency in lepidopterans and other RNAi-refractory pests

Visual impairment and age-related eye diseases in Florida: Findings from 2006 Behavioral Risk Factors Surveillance System (BRFSS) in Nine states

Yan Li, Amy Z Fan, Lina S BalluzBehavioral Surveillance Branch, Division of Adult and Community Health, National Center for Chronic Disease Prevention and Health Promotion, Centers for Disease Control and Prevention, Atlanta, GA, USAPurpose: To compare the prevalence of age-related eye disease, visual impairment, and eye care service utilization among adults aged 65 and older in Florida with eight other states. Methods: In 2006, nine states conducted the visual impairment and access to eye care module using the Behavioral Risk Factors Surveillance System (BRFSS) survey (N = 62,750). Visual impairment was based on self-reported ability to see distant and near objects. Age-related eye diseases including cataract, glaucoma, macular degeneration, and diabetic retinopathy were self-reported with diagnosis confirmed by a health care professional. Eye care visit or examination was assessed by whether a respondent reported an eye visit or dilated eye examination within the past year.Results: The estimated prevalence of distant and near visual impairment was lower in Florida than in the eight other states (distant: 11.5% vs 15.2%, P < 0.001; near: 22.3% vs 28.7%, P < 0.001). There was no significant difference with the prevalence of age-related macular degeneration and diabetic retinopathy between these two groups. The prevalence of glaucoma and cataract was higher in Florida. The rates of eye care visits (80.5% vs 74.8%, P < 0.01) and dilated eye examinations (74.7% vs 64.0%, P < 0.01) were higher in Florida. After controlling for demographic variables, chronic conditions, insurance, and eye examination, results for elderly in Florida continued to demonstrate less visually impaired.Conclusion: Fewer elderly in Florida reported visual impairment in spite of comparable or higher prevalence of age-related eye diseases with other states. Health care utilization and health insurance for eye care coverage were also higher in Florida, which may account for the phenomena. More research is needed to investigate the association.Keywords: visual impairment, age-related eye disease, eye care service utilization, health insurance, BRFS

Analisis Hubungan Shift Kerja dan Situasi Kerja dengan Kelelahan Kerja pada Security di PT XYZ Balikpapan

fatigue is a problem that often occurs in the workplace, including security workers. Fatigue is a process that results in a decrease in welfare, capacity or performance as a result of work activities caused by several factors, namely work shifts and work situations will cause fatigue. This study aims to determine whether there is a relationship between work shifts and work situations with fatigue in security at PT XYZ Balikpapan. The research method used a quantitative research using a cross sectional study design. The research instrument used the Industrial Fatigue Research Committee (IFRC) questionnaire. The results of this study were 20 security who worked in a shift system who had low fatigue as many as 20 people (40%), who had moderate fatigue as many as 15 people (30%), who had heavy fatigue as many as 11 people (22%), while who had very severe fatigue as many as 4 people (8%). For comfortable work situations as many as 45 people (90%), less comfortable as many as 5 people (10%), very uncomfortable none. The results of the analysis using the Spearman Rho test obtained a p value = 0.000 where p <0.05 which means there is a relationship between work shifts and fatigue and work situations obtained p value = 0,018 where p <0.05 which means there is a relationship between work situations with fatigue