Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer.

Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis

Elastoplastic Large Deflection Analysis of Cold-formed Members Using Spline Finite Strip Method

The elastoplastic large deflection behaviour of cold-formed members is analysed by a nonlinear spline finite strip method. The method is developed using the principle of virtual work, based on the total Lagrangian description. It is used to deal with problems of geometric and material nonlinearity. The displacement function of a strip is expressed as the product of transverse interpolation polynomials and longitudinal B3-splines. The effect of arbitrary initial imperfections is taken into consideration. The influence of cold-bending residual stress on the local and overall behaviour of cold-formed lipped angle columns is investigated especially. The numeric examples show that the method possesses such advantages as fewer degrees of freedom, fine continuity, good boundary adaptation, quick computation speed and high accuracy etc

N-(3,4-Dichloro­phen­yl)thio­urea

In the title compound, C7H6Cl2N2S, the benzene ring and the mean plane of the thio­urea fragment [—N—C(=S)—N] make a dihedral angle of 66.77 (3)°. Inter­molecular N—H⋯S and N—H⋯Cl hydrogen bonds link the mol­ecules into a three-dimensional network

Analytic solutions of relativistic dissipative spin hydrodynamics with radial expansion in Gubser flow

We have derived the analytic solutions of dissipative relativistic spin hydrodynamics with Gubser expansion. Following the standard strategy of deriving the solutions in a Gubser flow, we take the Weyl rescaling and obtain the energy-momentum and angular momentum conversation equations in the $dS_{3}\times\mathbb{R}$ space-time. We then derive the analytic solutions of spin density, spin potential and other thermodynamic in $dS_{3}\times\mathbb{R}$ space-time and transform them back into Minkowski space-time $\mathbb{R}^{3,1}$. In the Minkowski space-time, the spin density and spin potential including the information of radial expansion decay as $\sim L^{-2}\tau^{-1}$ and $\sim L^{-2}\tau^{-1/3}$ in large $L$ limit, with $\tau$ being proper time and $L$ being the characteristic length of the system, respectively. Moreover, we observe the non-vanishing spin corrections to the energy density and other dissipative terms in the Belinfante form of dissipative spin hydrodynamics. Our results can also be used as test beds for future simulations of relativistic dissipative spin hydrodynamics.Comment: 28 pages; 1 table and 1 figure are adde

Features-Based Deisotoping Method for Tandem Mass Spectra

For high-resolution tandem mass spectra, the determination of monoisotopic masses of fragment ions plays a key role in the subsequent peptide and protein identification. In this paper, we present a new algorithm for deisotoping the bottom-up spectra. Isotopic-cluster graphs are constructed to describe the relationship between all possible isotopic clusters. Based on the relationship in isotopic-cluster graphs, each possible isotopic cluster is assessed with a score function, which is built by combining nonintensity and intensity features of fragment ions. The non-intensity features are used to prevent fragment ions with low intensity from being removed. Dynamic programming is adopted to find the highest score path with the most reliable isotopic clusters. The experimental results have shown that the average Mascot scores and F-scores of identified peptides from spectra processed by our deisotoping method are greater than those by YADA and MS-Deconv software