On the medication distribution system for home health care through convenience stores, lockers, and home delivery.

Medication distribution service can be delivered based on a combination of home delivery and customer pickup. That is, medications are delivered either to customers' homes directly or to the pickup facilities (e.g. lockers) close to customers' homes. In Taiwan, there are more than 11,000 convenience stores that provide a 24-h service for customers to pick up the ordered items from e-commerce, which is unique to the world. In the medication distribution system, convenience stores can provide a unique opportunity for customers to more conveniently collect medications at stores, and also can reduce the operating cost for a logistics company providing the medication delivery service. Therefore, this work proposes a medication distribution system through convenience stores, lockers, and home delivery. Under this system, this work investigates how to simultaneously determine employment of convenience store chains, the convenience store locations to be visited, locations of lockers, vehicle routes for convenience stores and lockers, and vehicle routes for customers' homes, so that the total operating cost is minimized. This work further proposes a genetic algorithm to solve the medication distribution problem. Through simulation, the experimental results show that the proposed algorithm is able to solve the problem efficiently

Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei

Streptomyces species are highly abundant soil bacteria that possess linear chromosomes (and linear plasmids). The 5′ ends of these molecules are covalently bound by terminal proteins (TPs), that are important for integrity and replication of the telomeres. There are at least two types of TPs, both of which contain a DNA-binding domain and a classical eukaryotic nuclear localization signal (NLS). Here we show that the NLS motifs on these TPs are highly efficient in targeting the proteins along with covalently bound plasmid DNA into the nuclei of human cells. The TP-mediated nuclear targeting resembles the inter-kingdom gene transfer mediated by Ti plasmids of Agrobacterium tumefaciens, in which a piece of the Ti plasmid DNA is targeted to the plant nuclei by a covalently bound NLS-containing protein. The discovery of the nuclear localization functions of the Streptomyces TPs not only suggests possible inter-kingdom gene exchanges between Streptomyces and eukaryotes in soil but also provides a novel strategy for gene delivery in humans and other eukaryotes

A coordinated approach for supply-chain tracking in the liquefied natural gas industry

With the increased size and complexity of liquefied natural gas (LNG) projects, supplychain management has become a challenging process due to involvements of the remote location of the project site and the multiple stakeholders. The transparency and traceability of the supply-chain are critical as any surpluses or shortages of materials will put the project at risk. Currently, limited research has been conducted on LNG projects considering the total supply-chain perspective, which refers to all stages of materials tracking in off-site manufacturing, transportation, and site logistics. The purpose of this research is to propose a framework of a coordinated approach for supply-chain tracking in the LNG industry. Two focus group studies were organized to develop the proposed framework: One for LNG construction supply chain process development, and another for alternative tracking technologies selection. In addition, two experiments, namely off-site fabrication tracking and site logistics tracking, were conducted in a field to evaluate the feasibility of the proposed framework. Technology limitations were also discussed in terms of field implementation

Quantum rotor theory of spinor condensates in tight traps

In this work, we theoretically construct exact mappings of many-particle bosonic systems onto quantum rotor models. In particular, we analyze the rotor representation of spinor Bose-Einstein condensates. In a previous work it was shown that there is an exact mapping of a spin-one condensate of fixed particle number with quadratic Zeeman interaction onto a quantum rotor model. Since the rotor model has an unbounded spectrum from above, it has many more eigenstates than the original bosonic model. Here we show that for each subset of states with fixed spin F_z, the physical rotor eigenstates are always those with lowest energy. We classify three distinct physical limits of the rotor model: the Rabi, Josephson, and Fock regimes. The last regime corresponds to a fragmented condensate and is thus not captured by the Bogoliubov theory. We next consider the semiclassical limit of the rotor problem and make connections with the quantum wave functions through use of the Husimi distribution function. Finally, we describe how to extend the analysis to higher-spin systems and derive a rotor model for the spin-two condensate. Theoretical details of the rotor mapping are also provided here.Comment: 10 pages, 2 figure

A Simple, Secure and Universal Pancreaticojejunostomy following Pancreaticoduodenectomy

Although the operative mortality of pancreaticoduodenal resection has decreased recently, the operative morbidity resulting from a leaking pancreatic anastomosis remains high. We described our experience in 50 consecutive cases with a simple, secure end to side pancreaticojejunostomy. We used a paediatric nasogastric tube in the pancreatic remnant duct as a temporary external pancreatic drain. There were 29 men and 21 women ranging from 12 to 84 years with a median age of 61 years. Forty-two patients underwent a standard Whipple procedure and eight a pylorus preserving pancreaticoduodenectomy. Average operating time was 270 minutes with a range of 170 to 480 minutes. The pancreaticojejunostomy could be constructed in a mean of 8 minutes. Intraoperative blood loss ranged from 150 to 3500 mL with a mean of 910mL. Twenty-five patients (50 %) received no blood transfusion. The consistency of the pancreatic remnant was hard in 12 patients (24 %) and normal in 38 patients (76 %). The pancreatic duct was dilated (>4mm) in 15 patients (30 %). There was no operative mortality and only three (6.0 %) minor leaks from the pancreatic anastomosis which healed spontaneously. It was difficult to determine if the leaks were related to the consistency of the pancreatic remnant, the size of the pancreatic duct, the amount of intraoperative blood loss, operating time, sex of the patient or experience of the surgeon, as there were only three leaks. We concluded that our technique for pancreaticojejunal anastomosis following pancreaticoduodenectomy was safe and applicable to, standard Whipple or pylorus preserving pancreaticoduodenectomy, small or dilated pancreatic ducts, normal or fibrotic pancreas

A role of ygfZ in the Escherichia coli response to plumbagin challenge

Plumbagin is found in many herbal plants and inhibits the growth of various bacteria. Escherichia coli strains are relatively resistant to this drug. The mechanism of resistance is not clear. Previous findings showed that plumbagin treatment triggered up-regulation of many genes in E. coli including ahpC, mdaB, nfnB, nfo, sodA, yggX and ygfZ. By analyzing minimal inhibition concentration and inhibition zones of plumbagin in various gene-disruption mutants, ygfZ and sodA were found critical for the bacteria to resist plumbagin toxicity. We also found that the roles of YgfZ and SodA in detoxifying plumbagin are independent of each other. This is because of the fact that ectopically expressed SodA reduced the superoxide stress but not restore the resistance of bacteria when encountering plumbagin at the absence of ygfZ. On the other hand, an ectopically expressed YgfZ was unable to complement and failed to rescue the plumbagin resistance when sodA was perturbed. Furthermore, mutagenesis analysis showed that residue Cys228 within YgfZ fingerprint region was critical for the resistance of E. coli to plumbagin. By solvent extraction and HPLC analysis to follow the fate of the chemical, it was found that plumbagin vanished apparently from the culture of YgfZ-expressing E. coli. A less toxic form, methylated plumbagin, which may represent one of the YgfZ-dependent metabolites, was found in the culture supernatant of the wild type E. coli but not in the ΔygfZ mutant. Our results showed that the presence of ygfZ is not only critical for the E coli resistance to plumbagin but also facilitates the plumbagin degradation