Research on the Application Strategy of Virtual Reality Technology under the Background of Media Integration -- Take Chinese Minnan Culture Short Video as an Example

In this study, an experiment was designed to verify the communication effects and impacts of virtual reality technology on the viewers of a short video on Minnan cultural content. A control experiment and a questionnaire survey were chosen as the main research methods. According to the requirements of the experiment, the research subjects meeting the requirements were selected and divided into the control group and the experimental group. At the end of the experiment, a questionnaire survey was conducted on all the participants. The results and data of the questionnaire were analyzed at the end.By analyzing the data from the results of the questionnaire and with almost similar other variables in the control group, the minor effects of some subjective and objective factors on the experiment were excluded. The samples of the two groups showed significant differences in all eight items of the questionnaire, including the degree of interest in the content of the short videos, the richness of the content of the short videos, the rating of the degree of integration of the traditional culture and the short videos, the rating of the sense of visual experience and satisfaction with the content of this part of the short videos, the willingness to create this kind of short videos, the likelihood of recommending this kind of short videos to your family members or friends, the complete explanation of the connotation of traditional culture by using the short videos, and whether or not it will attract you to watch the similar content the next time, and the question of whether the traditional culture is fully explained through the short videos.It was found by analyzing the result data of this experiment and the questionnaire survey conducted after the experiment. Compared with Group B who watched the short video of Minnan culture supported by virtual reality technology, the audience of Group A had a better sense of video viewing and content experience, and gained a better viewing and using experience. This method makes traditional culture better disseminated and presented with the help of virtual reality technology. Therefore, we believe that combining virtual reality technology with short videos of Minnan culture can better spread the traditional culture of Minnan. And virtual reality technology has the characteristics of low cost and easy to operate. It is convenient for short video creators to choose and create

Using primary care records to evaluate the National Herpes Zoster Immunisation Program in Australia

Background: The National Herpes Zoster Immunisation Program using the one-dose live-attenuated zoster vaccine commenced in Australia in November 2016 for 70-year-olds with a catch-up program for 71–79-year-olds. As surveillance data to monitor the program effects was limited, this thesis presents four studies examining aspects of program evaluation including vaccine coverage, the impact of vaccination on zoster incidence, and vaccine effectiveness. Methods: MedicineInsight consists of de-identified electronic medical records extracted from participating general practices across Australia. Vaccination data from MedicineInsight records to December 2018 was used to estimate vaccine coverage and factors associated with vaccine receipt using a logistic regression model. The additional effects of a structured older persons health assessment and co-administration of seasonal influenza vaccines on zoster vaccination uptake were analysed using generalized estimating equations. An interrupted time-series model was used to investigate the impact of the program on zoster incidence and Cox proportional hazards models were used to estimate real-world vaccine effectiveness. Results: Twenty-six months following commencement of the national program, 46.9% (25,791/55,034) of those aged 70–79 years old had received a zoster vaccine. Factors associated with vaccine receipt included female sex, area of residence, and socioeconomic status. Both the structured older persons health assessment and co-administration of seasonal influenza vaccines were associated with higher zoster vaccine uptake (adjusted OR = 3.0 for both). The program was also significantly associated with reduced zoster incidence in those aged 70–79 years old, with an estimated annual decrease of 2.3 (95% CI: 1.3–3.2) per 1000 persons in the two years after the program was launched; an estimated 7000 zoster cases were prevented through the program. In the first year of the program, vaccine effectiveness against incident zoster was 63.5% (95% CI: 47.5–74.6) but this fell to 48.2% (95% CI: 30.0–61.7) in the second year. Conclusions: This thesis uses electronic general practice records in Australia to provide estimates of zoster vaccine coverage and adds to the evidence on the impact of vaccination on zoster disease and vaccine effectiveness. I found moderate levels of coverage and a level of effectiveness and reductions in disease consistent with international data

Research on the External Communication of Chinese Culture Empowered by Short Videos in Rural China -- Take the Short Video Content of YouTube Platform as an Example

Using YouTube as the platform and rural short videos as the entry point, this study investigates how Chinese rural short videos empower the external dissemination of Chinese culture on the YouTube platform. By using comparative analysis and content analysis research methods to select samples and construct categories, the aim is to compare rural and non rural themed Chinese short videos, as well as the similarities and differences in text structure and symbol types between Chinese rural short videos and foreign rural short videos, to analyze the empowerment of rural elements combined with different themes, and to summarize the dissemination content characteristics of Chinese rural short videos that have successfully gained popularity on the YouTube platform

Hyaluronan synthase mediates dye translocation across liposomal membranes

<p>Abstract</p> <p>Background</p> <p>Hyaluronan (HA) is made at the plasma membrane and secreted into the extracellular medium or matrix by phospolipid-dependent hyaluronan synthase (HAS), which is active as a monomer. Since the mechanism by which HA is translocated across membranes is still unresolved, we assessed the presence of an intraprotein pore within HAS by adding purified <it>Streptococcus equisimilis </it>HAS (SeHAS) to liposomes preloaded with the fluorophore Cascade Blue (CB).</p> <p>Results</p> <p>CB translocation (efflux) was not observed with mock-purified material from empty vector control <it>E. coli </it>membranes, but was induced by SeHAS, purified from membranes, in a time- and dose-dependent manner. CB efflux was eliminated or greatly reduced when purified SeHAS was first treated under conditions that inhibit enzyme activity: heating, oxidization or cysteine modification with N-ethylmaleimide. Reduced CB efflux also occurred with SeHAS K48E or K48F mutants, in which alteration of K48 within membrane domain 2 causes decreased activity and HA product size. The above results used liposomes containing bovine cardiolipin (BCL). An earlier study testing many synthetic lipids found that the best activating lipid for SeHAS is tetraoleoyl cardiolipin (TO-CL) and that, in contrast, tetramyristoyl cardiolipin (TM-CL) is an inactivating lipid (Weigel et al, J. <it>Biol. Chem</it>. <b>281</b>, 36542, 2006). Consistent with the effects of these CL species on SeHAS activity, CB efflux was more than 2-fold greater in liposomes made with TO-CL compared to TM-CL.</p> <p>Conclusions</p> <p>The results indicate the presence of an intraprotein pore in HAS and support a model in which HA is translocated to the exterior by HAS itself.</p

Maternal-Infant Correlation of Multidrug-Resistant Staphylococcus aureus Carriage: A Prospective Cohort Study

Objectives: We aim to assess the correlation of multidrug-resistant Staphylococcus aureus (MDR S. aureus) carriage between mothers and their newborn infants.Materials and Methods: We conducted a prospective cohort study of mothers and their newborn infants in two hospitals in Shenzhen, China, from August to November 2015. We collected demographic and clinical information from mothers and newborn infants by face-to-face questionnaires and medical datasets. Serial swabs were collected from mothers and their newborn infants for further experiments. Maternal-infant correlation was assessed using the Poisson regression model.Results: The prevalence of MDR S. aureus vaginal carriage in mothers was 4.7% (86/1834). The incidence of MDR S. aureus carriage in newborn infants was 1.3% (23/1834). The adjusted relative risk and 95% confidence interval of maternal-infant MDR S. aureus carriage was 7.63 (2.99–19.49). Six MDR S. aureus maternal-infant pairs were concordant. The phenotypic and molecular characteristics of MDR S. aureus isolates were similar between mothers and their newborn infants.Conclusion: MDR S. aureus vaginal carriage in mothers was associated with an increased risk for MDR S. aureus carriage in their newborn infants

The global landscape of approved antibody therapies

Antibody therapies have become an important class of therapeutics in recent years as they have exhibited outstanding efficacy and safety in the treatment of several major diseases including cancers, immune-related diseases, infectious disease and hematological disease. There has been significant progress in the global research and development landscape of antibody therapies in the past decade. In this review, we have collected available data from the Umabs Antibody Therapies Database (Umabs-DB, https://umabs.com) as of 30 June 2022. The Umabs-DB shows that 162 antibody therapies have been approved by at least one regulatory agency in the world, including 122 approvals in the US, followed by 114 in Europe, 82 in Japan and 73 in China, whereas biosimilar, diagnostic and veterinary antibodies are not included in our statistics. Although the US and Europe have been at the leading position for decades, rapid advancement has been witnessed in Japan and China in the past decade. The approved antibody therapies include 115 canonical antibodies, 14 antibody-drug conjugates, 7 bispecific antibodies, 8 antibody fragments, 3 radiolabeled antibodies, 1 antibody-conjugate immunotoxin, 2 immunoconjugates and 12 Fc-Fusion proteins. They have been developed against 91 drug targets, of which PD-1 is the most popular, with 14 approved antibody-based blockades for cancer treatment in the world. This review outlined the global landscape of the approved antibody therapies with respect to the regulation agencies, therapeutic targets and indications, aiming to provide an insight into the trends of the global development of antibody therapies