2 nd Brazilian Consensus on Chagas Disease, 2015

Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research

Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Cichla monoculus is an endemic species from Amazon basin and one of the main predators in the Amazon Rivers. It is widely commercialized as food, becoming one of the main items in Amazonian fisheries. Despite its economic importance, there are few genetic population studies in its natural environment. The present contribution describes 12 microsatellite loci used to analyze 27 individuals of C. monoculus. The number of alleles for each locus ranged from 2 to 13. The observed (H(O)) and expected (H(E)) heterozygosity values ranged from 0.45 to 0.94 and 0.45 to 0.90, respectively. Out of 12 polymorphic loci, nine did not deviate from Hardy-Weinberg Equilibrium after Bonferroni correction. A cross-amplification test with five different species suggests potential transferability of all analyzed loci.21215218Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPEAMConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT ADAPTACoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq