Chromosome analysis in Pseudopaludicola (Anura, Leiuperidae), with description of sex chromosomes XX/XY in P-saltica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Taxonomic changes have frequently occurred in the anuran genus Pseudopaludicola as a consequence of high morphological similarity among its species. The present work reports karyotypic analysis of three Pseudopaludicola species sampled in their type locality and four Pseudopaludicola populations from distinct localities, aiming at contributing to the systematics of this genus. Chromosomes were stained with Giemsa or submitted to the silver staining (Ag-NOR) and C-banding techniques. The karyotype was 2n=22 in P. mineira, Pseudopaludicola sp. and two populations of P. saltica. The chromosome pair 8 was heteromorphic in P. saltica, characterizing a XX/XY sex-determination system with telocentric X and submetacentric Y. Highly similar karyotypes with 2n=18 chromosomes were observed in P. canga, P. aff. canga from Barreirinhas, State of Maranhao, Uberlandia, State of Minas Gerais and Icem, State Sao Paulo. The high similarity among the karyotypes 2n=18 suggested that the populations of P. aff. canga belong to the group 'pusilla', the same group of P. canga. The data demonstrated also that P. aff. canga from Barreirinhas (northeast region) is cytogenetically identical to P. canga with regarding the NOR site position in pair 3 and the presence of a heterochromatic block in the pair 2, whereas P. aff. canga from Uberlandia and Icem (southeast) had the NOR in the pair 9. Moreover, the cytogenetic data discriminated P. mineira and Pseudopaludicola sp. from the previously analyzed species with 22 chromosomes, and suggested that Pseudopaludicola sp. is an undescribed species. Sexual heteromorphic chromosomes are firstly reported in Pseudopaludicola and the data indicated the need of an extensive taxonomic review in this genus.14724352Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundacao de Ensino de Sao Paulo (FUNDESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP [6/60055-0

Satisfação dos usuários assistidos nos Centros de Especialidades Odontológicas do Município do Recife, Pernambuco, Brasil Patient satisfaction at Specialized Dental Clinics in Recife, Pernambuco State, Brazil

O estudo objetivou avaliar a satisfação dos usuários assistidos nos Centros de Especialidades Odontológicas do Município do Recife, Pernambuco, Brasil. Realizou-se entrevista pessoal padronizada com 272 pacientes utilizando o Questionário de Avaliação da Qualidade dos Serviços de Saúde Bucal (QASSaB). Os resultados indicaram uma avaliação positiva para a maioria das dimensões no geral. A acessibilidade foi um ponto crítico destacado. Houve diferença significante de 5% na satisfação dos usuários, entre as unidades de serviço, para: relações humanas/cirurgião-dentista (p = 0,001); relações humanas/auxiliar (p = 0, 007); eficácia (p = 0,014); acessibilidade/tempo de espera (p = 0,018); ambiente físico/limpeza da recepção (p = 0,004); aceitabilidade (p = 0,029); efetividade/aparência dos dentes (p = 0,029) e efetividade/capacidade de mastigação (p < 0,001). Concluiu-se que a maioria dos usuários está satisfeita com a assistência prestada nos centros e que a satisfação difere em cada centro.<br>This study evaluated patient satisfaction at four specialized dental clinics in Recife, Pernambuco State, Brazil. Data were collected through a standardized self-applied individual questionnaire with 272 patients, to evaluate user satisfaction with public dental services. The findings indicated a positive evaluation on most dimensions, except for accessibility. Significant differences were observed between the services on the following dimensions: patient-dentist relationship (p = 0.001); patient-dental assistant relationship (p = 0.007); effectiveness (p = 0.014) accessibility/waiting time (p = 0.018); physical environment/reception room cleanness (p = 0.004); acceptability (p = 0.029); and effectiveness/tooth appearance (p = 0.029), effectiveness/chewing capacity (p < 0.001). In conclusion, most users were satisfied with the dental care, and satisfaction differed between services