Impact of Pulp on the Chemical Profile of Mango Wine

The aim of this study was to assess the effect of mango pulp inclusion (which mimicked the maceration stepin grape wine fermentation) on the fermentation dynamics and chemical profile of mango wine, especiallythe volatiles. The growth of Saccharomyces cerevisiae MERIT.ferm was slower in the mango juice with pulp(uncentrifuged juice), with a corresponding slower reduction in °Brix, relative to the juice without pulp(centrifuged juice). The utilisation of glucose, fructose and sucrose was similar in both uncentrifuged andcentrifuged juices, with almost complete consumption. Citric, tartaric, malic, pyruvic and succinic acidwere the major organic acids in the wines fermented from both the uncentrifuged and centrifuged juices.Citric acid decreased slightly in the macerated wine. Tartaric and malic acid decreased in both wines.Pyruvic acid increased slightly and succinic acid remained almost constant in both wines. Monoterpenes,as one of the signature aroma compounds in mango juice, decreased dramatically in both wines, but wereten times higher in the macerated wine. Terpenols were at least four times higher in the macerated wine.The macerated wine also produced higher levels of fusel alcohols and acetate esters compared to the nonmaceratedwine. On the other hand, the non-macerated wine possessed a higher concentration of mediumchainfatty acids and corresponding ethyl esters. This study indicates that the inclusion of pulp in mangowine fermentation would contribute to the aroma complexity

Upconversion Nanoparticle-Based Cell Membrane-Coated cRGD Peptide Bioorthogonally Labeled Nanoplatform for Glioblastoma Treatment

Glioblastoma is hard to be eradicated partly because of the obstructive blood-brain barrier (BBB) and the dynamic autophagy activities of glioblastoma. Here, hydroxychloroquine (HDX)-loaded yolk-shell upconversion nanoparticle (UCNP)@Zn0.5Cd0.5S nanoparticle coating with the cyclic Arg-Gly-Asp (cRGD)-grafted glioblastoma cell membrane for near-infrared (NIR)-triggered treatment of glioblastoma is prepared for the first time. [email protected] (abbreviated as YSN, yolk-shell nanoparticle) under NIR radiation will generate reactive oxygen species for imposing cytotoxicity. HDX, the only available autophagy inhibitor in clinical studies, can enhance cytotoxicity by preventing damaged organelles from being recycled. The cRGD-decorated cell membrane allowed the HDX-loaded nanoparticles to efficiently bypass the BBB and specifically target glioblastoma cells. Exceptional treatment efficacy of the NIR-triggered chemotherapy and photodynamic therapy was achieved in U87 cells and in the mouse glioblastoma model as well. Our results provided proof-of-concept evidence that HDX@YSN@CCM@cRGD could overcome the delivery barriers and achieve targeted treatment of glioblastoma

Protective Enterotoxigenic Escherichia coli Antigens in a Murine Intranasal Challenge Model

Citation: Kumar, A., Hays, M., Lim, F., Foster, L. J., Zhou, M. X., Zhu, G. Q., . . . Hardwidge, P. R. (2015). Protective Enterotoxigenic Escherichia coli Antigens in a Murine Intranasal Challenge Model. Plos Neglected Tropical Diseases, 9(8), 16. doi:10.1371/journal.pntd.0003924Enterotoxigenic Escherichia coli (ETEC) is an endemic health threat in underdeveloped nations. Despite the significant effort extended to vaccine trials using ETEC colonization factors, these approaches have generally not been especially effective in mediating cross-protective immunity. We used quantitative proteomics to identify 24 proteins that differed in abundance in membrane protein preparations derived from wild-type vs. a type II secretion system mutant of ETEC. We expressed and purified a subset of these proteins and identified nine antigens that generated significant immune responses in mice. Sera from mice immunized with either the MltA-interacting protein MipA, the periplasmic chaperone seventeen kilodalton protein, Skp, or a long-chain fatty acid outer membrane transporter, ETEC_2479, reduced the adherence of multiple ETEC strains differing in colonization factor expression to human intestinal epithelial cells. In intranasal challenge assays of mice, immunization with ETEC_ 2479 protected 88% of mice from an otherwise lethal challenge with ETEC H10407. Immunization with either Skp or MipA provided an intermediate degree of protection, 68 and 64%, respectively. Protection was significantly correlated with the induction of a secretory immunoglobulin A response. This study has identified several proteins that are conserved among heterologous ETEC strains and may thus potentially improve cross- protective efficacy if incorporated into future vaccine designs

Influence of Arctic sea ice on European summer precipitation

Copyright © 2013 IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0/). Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.Open Access journalThe six summers from 2007 to 2012 were all wetter than average over northern Europe. Although none of these individual events are unprecedented in historical records, the sequence of six consecutive wet summers is extraordinary. Composite analysis reveals that observed wet summer months in northern Europe tend to occur when the jet stream is displaced to the south of its climatological position, whereas dry summer months tend to occur when the jet stream is located further north. Highly similar mechanisms are shown to drive simulated precipitation anomalies in an atmospheric model. The model is used to explore the influence of Arctic sea ice on European summer climate, by prescribing different sea ice conditions, but holding other forcings constant. In the simulations, Arctic sea ice loss induces a southward shift of the summer jet stream over Europe and increased northern European precipitation. The simulated precipitation response is relatively small compared to year-to-year variability, but is statistically significant and closely resembles the spatial pattern of precipitation anomalies in recent summers. The results suggest a causal link between observed sea ice anomalies, large-scale atmospheric circulation and increased summer rainfall over northern Europe. Thus, diminished Arctic sea ice may have been a contributing driver of recent wet summers.UK Natural Environment Research Council (NERC

Duality Cascade in Brane Inflation

We show that brane inflation is very sensitive to tiny sharp features in extra dimensions, including those in the potential and in the warp factor. This can show up as observational signatures in the power spectrum and/or non-Gaussianities of the cosmic microwave background radiation (CMBR). One general example of such sharp features is a succession of small steps in a warped throat, caused by Seiberg duality cascade using gauge/gravity duality. We study the cosmological observational consequences of these steps in brane inflation. Since the steps come in a series, the prediction of other steps and their properties can be tested by future data and analysis. It is also possible that the steps are too close to be resolved in the power spectrum, in which case they may show up only in the non-Gaussianity of the CMB temperature fluctuations and/or EE polarization. We study two cases. In the slow-roll scenario where steps appear in the inflaton potential, the sensitivity of brane inflation to the height and width of the steps is increased by several orders of magnitude comparing to that in previously studied large field models. In the IR DBI scenario where steps appear in the warp factor, we find that the glitches in the power spectrum caused by these sharp features are generally small or even unobservable, but associated distinctive non-Gaussianity can be large. Together with its large negative running of the power spectrum index, this scenario clearly illustrates how rich and different a brane inflationary scenario can be when compared to generic slow-roll inflation. Such distinctive stringy features may provide a powerful probe of superstring theory.Comment: Corrections in Eq.(5.47), Eq (5.48), Eq(5.49) and Fig

Reversible room-temperature ferromagnetism in Nb-doped SrTiO3 single crystals

The search for oxide-based room-temperature ferromagnetism has been one of the holy grails in condensed matter physics. Room-temperature ferromagnetism observed in Nb-doped SrTiO3 single crystals is reported in this Rapid Communication. The ferromagnetism can be eliminated by air annealing (making the samples predominantly diamagnetic) and can be recovered by subsequent vacuum annealing. The temperature dependence of magnetic moment resembles the temperature dependence of carrier density, indicating that the magnetism is closely related to the free carriers. Our results suggest that the ferromagnetism is induced by oxygen vacancies. In addition, hysteretic magnetoresistance was observed for magnetic field parallel to current, indicating that the magnetic moments are in the plane of the samples. The x-ray photoemission spectroscopy, the static time-of-flight and the dynamic secondary ion mass spectroscopy and proton induced x-ray emission measurements were performed to examine magnetic impurities, showing that the observed ferromagnetism is unlikely due to any magnetic contaminant.Comment: 6 pages, 6 figure

Cyclic cosmology from Lagrange-multiplier modified gravity

We investigate cyclic and singularity-free evolutions in a universe governed by Lagrange-multiplier modified gravity, either in scalar-field cosmology, as well as in $f(R)$ one. In the scalar case, cyclicity can be induced by a suitably reconstructed simple potential, and the matter content of the universe can be successfully incorporated. In the case of $f(R)$-gravity, cyclicity can be induced by a suitable reconstructed second function $f_2(R)$ of a very simple form, however the matter evolution cannot be analytically handled. Furthermore, we study the evolution of cosmological perturbations for the two scenarios. For the scalar case the system possesses no wavelike modes due to a dust-like sound speed, while for the $f(R)$ case there exist an oscillation mode of perturbations which indicates a dynamical degree of freedom. Both scenarios allow for stable parameter spaces of cosmological perturbations through the bouncing point.Comment: 8 pages, 3 figures, references added, accepted for publicatio

Comparing Brane Inflation to WMAP

We compare the simplest realistic brane inflationary model to recent cosmological data, including WMAP 3-year cosmic microwave background (CMB) results, Sloan Digital Sky Survey luminous red galaxies (SDSS LRG) power spectrum data and Supernovae Legacy Survey (SNLS) Type 1a supernovae distance measures. Here, the inflaton is simply the position of a $D3$-brane which is moving towards a $\bar{D}3$-brane sitting at the bottom of a throat (a warped, deformed conifold) in the flux compactified bulk in Type IIB string theory. The analysis includes both the usual slow-roll scenario and the Dirac-Born-Infeld scenario of slow but relativistic rolling. Requiring that the throat is inside the bulk greatly restricts the allowed parameter space. We discuss possible scenarios in which large tensor mode and/or non-Gaussianity may emerge. Here, the properties of a large tensor mode deviate from that in the usual slow-roll scenario, providing a possible stringy signature. Overall, within the brane inflationary scenario, the cosmological data is providing information about the properties of the compactification of the extra dimensions.Comment: 45 pages 11 figure