20 research outputs found
Identification of Spanish isolates of Rhizoctonia solani from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
Anastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including
the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia
solani collected from Spain and Finland. There was a high similarity between the results obtained with the three
techniques. RAMS markers revealed more genetic variation among isolates of R. solani than RFLP. The anastomosis
group (AG)–3 isolates were clearly separated from isolates belonging to other AGs by RAMS, RFLPs and anastomosis
grouping. Almost all the isolates sampled from potato belonged to AG–3. No differences were observed between
Spanish and Finnish AG–3 isolates
Reactivation of latent bovine herpesvirus type 5 in cattle with polioencephalomalacia induced by ammonium sulphate
Identification of a uninucleate Rhizoctonia sp. by pathogenicity hyphal anastomosis and RAPD analysis
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Anamorphic and teleomorphic characteristics of a uninucleate Rhizoctonia sp. isolated from the roots of nursery grown conifer seedlings
Identification of Spanish isolates of Rhizoctonia solani from potato by anastomosis grouping, ITS-RLFP and RAMS-fingerprinting
Expression of Active, Secreted Human Prostate-specific Antigen by Recombinant Baculovirus-infected Insect Cells on a Pilot-scale
Potential role of multiple members of the kallikrein-related peptidase family of serine proteases in activating latent TGFβ1 in semen
Induced Liver Fibrosis Is Accompanied in Young and Old Animals by Age-Dependent Changes in Bone Marrow Cells
Oxygen targets and 6-month outcome after out of hospital cardiac arrest: a pre-planned sub-analysis of the targeted hypothermia versus targeted normothermia after Out-of-Hospital Cardiac Arrest (TTM2) trial.
Optimal oxygen targets in patients resuscitated after cardiac arrest are uncertain. The primary aim of this study was to describe the values of partial pressure of oxygen values (PaO <sub>2</sub> ) and the episodes of hypoxemia and hyperoxemia occurring within the first 72 h of mechanical ventilation in out of hospital cardiac arrest (OHCA) patients. The secondary aim was to evaluate the association of PaO <sub>2</sub> with patients' outcome.
Preplanned secondary analysis of the targeted hypothermia versus targeted normothermia after OHCA (TTM2) trial. Arterial blood gases values were collected from randomization every 4 h for the first 32 h, and then, every 8 h until day 3. Hypoxemia was defined as PaO <sub>2</sub> < 60 mmHg and severe hyperoxemia as PaO <sub>2</sub> > 300 mmHg. Mortality and poor neurological outcome (defined according to modified Rankin scale) were collected at 6 months.
1418 patients were included in the analysis. The mean age was 64 ± 14 years, and 292 patients (20.6%) were female. 24.9% of patients had at least one episode of hypoxemia, and 7.6% of patients had at least one episode of severe hyperoxemia. Both hypoxemia and hyperoxemia were independently associated with 6-month mortality, but not with poor neurological outcome. The best cutoff point associated with 6-month mortality for hypoxemia was 69 mmHg (Risk Ratio, RR = 1.009, 95% CI 0.93-1.09), and for hyperoxemia was 195 mmHg (RR = 1.006, 95% CI 0.95-1.06). The time exposure, i.e., the area under the curve (PaO <sub>2</sub> -AUC), for hyperoxemia was significantly associated with mortality (p = 0.003).
In OHCA patients, both hypoxemia and hyperoxemia are associated with 6-months mortality, with an effect mediated by the timing exposure to high values of oxygen. Precise titration of oxygen levels should be considered in this group of patients.
clinicaltrials.gov NCT02908308 , Registered September 20, 2016