The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility

Cyclin-dependent kinases (Cdks) fulfill key functions in many cellular processes, including cell cycle progression and cytoskeletal dynamics. A limited number of Cdk substrates have been identified with few demonstrated to be regulated by Cdk-dependent phosphorylation. We identify on protein expression arrays novel cyclin E–Cdk2 substrates, including SIRT2, a member of the Sirtuin family of NAD+-dependent deacetylases that targets α-tubulin. We define Ser-331 as the site phosphorylated by cyclin E–Cdk2, cyclin A–Cdk2, and p35–Cdk5 both in vitro and in cells. Importantly, phosphorylation at Ser-331 inhibits the catalytic activity of SIRT2. Gain- and loss-of-function studies demonstrate that SIRT2 interfered with cell adhesion and cell migration. In postmitotic hippocampal neurons, neurite outgrowth and growth cone collapse are inhibited by SIRT2. The effects provoked by SIRT2, but not those of a nonphosphorylatable mutant, are antagonized by Cdk-dependent phosphorylation. Collectively, our findings identify a posttranslational mechanism that controls SIRT2 function, and they provide evidence for a novel regulatory circuitry involving Cdks, SIRT2, and microtubules

Практический опыт развития комплексной системы экологического просвещения в образовательной организации

В статье представлены разработанные и апробированные в образовательной организации новые подходы, механизмы и инструменты по одному из приоритетных направлений развития страны в части формирования экологического культуры вузовской молодежи с целью повышения познавательной активности, уровня экологических знаний в области гармоничного развития человека и природы, устойчивого интереса к экологическим проблемам современности, воспитания и привития бережного отношения к окружающей природе.The article presents new approaches, mechanisms and tools developed and tested in the educational organization in one of the priority directions of the country's development in terms of forming the ecological culture of university youth in order to increase cognitive activity, the level of ecological knowledge in the field of harmonious development of man and nature, and a steady interest in environmental problems of modernity, upbringing and inculcation of respect for nature

Mögliche Ermittlung der Messunsicherheit bei mikrobiologischen Analysen in Wasserproben

Die Anforderungen an die Qualität der Messergebnisse steigen stetig. Insbesondere die Bestimmung der Messunsicherheit mikrobiologischer Analyseergebnisse, die von einigen Labors durchgeführt wird, aber noch nicht vorgeschrieben ist, stellt viele Mikrobiologielabors vor eine große Herausforderung. Ziel ist eine Vorgehensweise, die sowohl möglichst genau als auch zeit- und kosteneffizient ist