Loção capilar a base de compostos bioativos extraídos de Ganoderma lucidum

Orientador: Prof. Dr. Carlos Ricardo SoccolCoorientadora: Dra. Andrea Thomaz Soccol ScholzTese (doutorado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Defesa : Curitiba, 23/03/2018Inclui referências: p.101-106Resumo: A alopecia, conhecida popularmente como queda capilar, atinge tanto homens quanto mulheres, em todas as faixas etárias. Sua incidência tem aumentado com o ritmo de vida cada vez mais estressante. É a doença com maior impacto psicossocial, levando a casos extremos até mesmo ao suicídio. A alopecia pode ocorrer devido a diversos fatores, sendo que a ação hormonal andrógena de diidrotestosterona (DHT) é a causa mais frequente. Esse hormônio é responsável pela miniaturização do folículo capilar e consequente queda dos fios de cabelo. O fungo Ganoderma lucidum é amplamente utilizado na medicina chinesa por suas propriedades anticancerígenas. No câncer de próstata, ele atua inibindo a enzima 5-a-redutase, responsável pela conversão de testosterona em DHT. Com base nessas informações, esporos de G. lucidum foram rompidos e seu material foi extraído em solução aquosa. Essa solução foi utilizada no desenvolvimento de uma loção capilar para tratamento de alopecia. A avaliação da loção capilar foi feita em duas etapas. Na primeira testou-se a loção em modelo experimental usando camundongos C57BL/6. Foram avaliados crescimento, desenvolvimento dos folículos e atividade hormonal em machos e fêmeas dos animais.macro e microscopicamente. Neste modelo constatou-se aumento superior a 50% na quantidade de folículos, e de até 10 vezes o diâmetro dos mesmos. Em machos as análises hormonais indicaram alterações não significativas (p>0,05) dos níveis de testosterona, decréscimo significativo (p<0,05) para DHT e aumento dos níveis de estradiol (p<0,05). Para as fêmeas, a única resposta hormonal diferente foi a queda dos níveis de testosterona (p<0,05). A segunda etapa consistiu em avaliar a loção em homens e mulheres. Os mesmos parâmetros realizados no modelo experimental foram aqui avaliados. Pôde-se observar o surgimento de novos folículos capilares, melhora da queda capilar, aumento da haste dos fios de cabelo, tornando-os mais fortes e resistentes, brilhosos e hidratados, com aceitação da fórmula por 99% dos pacientes. A loção capilar formulada a partir do extrato de esporos de G. lucidum mostrou ser um cosmético de alto potencial tecnológico e industrial. Palavras-chave: Alopecia. Folículos capilares. Loção capilar. Ganoderma lucidum. 5-a-redutase. Testosterona. Diidrotestosterona.Abstract: Alopecia, popularly known as hair loss, affects both men and women in all age groups. Its incidence has increased with the increasingly stressful of lifestyle. It is the disease with the greatest psychosocial impact, leading to extreme cases even to suicide. Alopecia can occur due to several factors, and the androgenic hormone action of dihydrotestosterone (DHT) is the most frequent cause. This hormone is responsible for the miniaturization of the hair follicle and consequent fall of hair strands. The fungus Ganoderma lucidum is widely used in Chinese medicine for its anticancer properties. In prostate cancer, it works by inhibiting the enzyme 5-a-reductase, responsible for the conversion of testosterone to DHT. Based on this information, spores of G. lucidum were disrupted and their material was extracted in aqueous solution. This solution was used in the development of a hair lotion for the treatment of alopecia. The evaluation of capillary lotion was done in two stages. In the first experiment the lotion was tested in an experimental model using C57BL/6 mice. Growth, follicle development and hormonal activity were evaluated in males and females of the animals, machro and microscopically. In this model, an increase of more than 50% in the number of follicles and up to 10 times the diameter of the follicles was observed. In males, hormonal analyzes indicated non-significant changes (p> 0.05) in testosterone levels, a significant decrease (p <0.05) for DHT and an increase in estradiol levels (p <0.05). For females, the only different hormonal response was the decrease in testosterone levels (p <0.05). The second stage consisted in evaluating lotion in men and women. The same parameters performed in the experimental model were evaluated here. It was observed the growth of new hair follicles, improved hair loss, hair shaft enlargement, making them stronger and resistant, shiny and hydrated, with acceptance of the formula by 99% of the patients. The hair lotion formulated from the spore extract of G. lucidum showed to be a cosmetic of high technological and industrial potential. Key words: Alopecia. Hair follicles. Hair lotion. Ganoderma lucidum. 5-a-reductase. Testosterone. Dihydrotestosterone

Biotechnological Production of Carotenoids and Their Applications in Food and Pharmaceutical Products

Pigments can be divided into four categories: natural, nature-identical, synthetic, and inorganic colors. Artificial colorants are the most used in food and pharmaceutical industries because of their advantages related to color range, price, resistance to oxygen degradation, and solubility. However, many natural pigments present health-promoting activities that make them an interesting option for human use and consumption. Natural colorants are derived from sources such as plants, insects, and microorganisms. Carotenoids are natural pigments with important biological activities, such as antioxidant and pro-vitamin A activity, that can be either extracted from plants and algae or synthesized by various microorganisms, including bacteria, yeasts, filamentous fungi, and microalgae. Advantages of microbial production include the ability of microorganisms to use a wide variety of low cost substrates, the better control of cultivation, and the minimized production time. After fermentation, carotenoids are usually recovered by cell disruption, solvent extraction, and concentration. Subsequent purification steps are followed depending on the application. The most prominent industrial applications of carotenoids, considering their health benefits, are in the food, feed, and pharmaceutical industries

Loção capilar a base de compostos bioativos extraídos de Ganoderma lucidum

Orientador: Prof. Dr. Carlos Ricardo SoccolCoorientadora: Dra. Andrea Thomaz Soccol ScholzTese (doutorado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Defesa : Curitiba, 23/03/2018Inclui referências: p.101-106Resumo: A alopecia, conhecida popularmente como queda capilar, atinge tanto homens quanto mulheres, em todas as faixas etárias. Sua incidência tem aumentado com o ritmo de vida cada vez mais estressante. É a doença com maior impacto psicossocial, levando a casos extremos até mesmo ao suicídio. A alopecia pode ocorrer devido a diversos fatores, sendo que a ação hormonal andrógena de diidrotestosterona (DHT) é a causa mais frequente. Esse hormônio é responsável pela miniaturização do folículo capilar e consequente queda dos fios de cabelo. O fungo Ganoderma lucidum é amplamente utilizado na medicina chinesa por suas propriedades anticancerígenas. No câncer de próstata, ele atua inibindo a enzima 5-a-redutase, responsável pela conversão de testosterona em DHT. Com base nessas informações, esporos de G. lucidum foram rompidos e seu material foi extraído em solução aquosa. Essa solução foi utilizada no desenvolvimento de uma loção capilar para tratamento de alopecia. A avaliação da loção capilar foi feita em duas etapas. Na primeira testou-se a loção em modelo experimental usando camundongos C57BL/6. Foram avaliados crescimento, desenvolvimento dos folículos e atividade hormonal em machos e fêmeas dos animais.macro e microscopicamente. Neste modelo constatou-se aumento superior a 50% na quantidade de folículos, e de até 10 vezes o diâmetro dos mesmos. Em machos as análises hormonais indicaram alterações não significativas (p>0,05) dos níveis de testosterona, decréscimo significativo (p<0,05) para DHT e aumento dos níveis de estradiol (p<0,05). Para as fêmeas, a única resposta hormonal diferente foi a queda dos níveis de testosterona (p<0,05). A segunda etapa consistiu em avaliar a loção em homens e mulheres. Os mesmos parâmetros realizados no modelo experimental foram aqui avaliados. Pôde-se observar o surgimento de novos folículos capilares, melhora da queda capilar, aumento da haste dos fios de cabelo, tornando-os mais fortes e resistentes, brilhosos e hidratados, com aceitação da fórmula por 99% dos pacientes. A loção capilar formulada a partir do extrato de esporos de G. lucidum mostrou ser um cosmético de alto potencial tecnológico e industrial. Palavras-chave: Alopecia. Folículos capilares. Loção capilar. Ganoderma lucidum. 5-a-redutase. Testosterona. Diidrotestosterona.Abstract: Alopecia, popularly known as hair loss, affects both men and women in all age groups. Its incidence has increased with the increasingly stressful of lifestyle. It is the disease with the greatest psychosocial impact, leading to extreme cases even to suicide. Alopecia can occur due to several factors, and the androgenic hormone action of dihydrotestosterone (DHT) is the most frequent cause. This hormone is responsible for the miniaturization of the hair follicle and consequent fall of hair strands. The fungus Ganoderma lucidum is widely used in Chinese medicine for its anticancer properties. In prostate cancer, it works by inhibiting the enzyme 5-a-reductase, responsible for the conversion of testosterone to DHT. Based on this information, spores of G. lucidum were disrupted and their material was extracted in aqueous solution. This solution was used in the development of a hair lotion for the treatment of alopecia. The evaluation of capillary lotion was done in two stages. In the first experiment the lotion was tested in an experimental model using C57BL/6 mice. Growth, follicle development and hormonal activity were evaluated in males and females of the animals, machro and microscopically. In this model, an increase of more than 50% in the number of follicles and up to 10 times the diameter of the follicles was observed. In males, hormonal analyzes indicated non-significant changes (p> 0.05) in testosterone levels, a significant decrease (p <0.05) for DHT and an increase in estradiol levels (p <0.05). For females, the only different hormonal response was the decrease in testosterone levels (p <0.05). The second stage consisted in evaluating lotion in men and women. The same parameters performed in the experimental model were evaluated here. It was observed the growth of new hair follicles, improved hair loss, hair shaft enlargement, making them stronger and resistant, shiny and hydrated, with acceptance of the formula by 99% of the patients. The hair lotion formulated from the spore extract of G. lucidum showed to be a cosmetic of high technological and industrial potential. Key words: Alopecia. Hair follicles. Hair lotion. Ganoderma lucidum. 5-a-reductase. Testosterone. Dihydrotestosterone

Torularhodin and Torulene: Bioproduction, Properties and Prospective Applications in Food and Cosmetics - a Review

Torularhodin and torulene are two widespread microbial carotenoids with relatively few studies, as compared to other nutraceutical carotenoids such as β-carotene, lycopene and astaxanthin. Several genera of microorganisms produce it in high concentration (up to 0.1% of the cell dry weight), probably as a protection against photooxidation and free radicals. These pigments, which differ by a terminal carboxylic group, have provitamin-A activity and, being red, have potential use as food and cosmetic color additives. Several factors affect the biosynthesis of these substances, including: the composition of culture media, light irradiation, which may enhance the carotenoid production up to 25% of the non-irradiated cultures, and temperature, which changes the carotenoid balance towards more of the acidic carotenoid (torularhodin) or the hydrocarbon (torulene). The biomass may be directly extracted using non polar solvents such as hexane or a hexane-acetone mixture, without need of cell disruption. Extensive purification is not needed for using the pigments as food or cosmetic additives, but it is still necessary to evaluate the bioactivity of the pigments in humans