Comparing the Effects of Conventional and Pastured Poultry Production Systems on the Stress Levels of Broilers

Abstract The objective of this study was to measure stress levels in broilers raised on a pasture production system (PPS) and a conventional production system (CPS) by evaluating lymphoid organ weights, white blood cell profiles (WBCP), total white blood cell counts (TWBC), and heterophil to lymphocyte ratios (HLR). Broilers were brooded indoors for 3 weeks then divided into 2 groups; one placed on pasture while the other remained indoors. Blood was collected at weeks 3, 5, and 7 via brachial venipuncture. After 49 days, birds were slaughtered and lymphoid organs harvested. Results showed no differences between treatments for lymphoid organs, TWBC, or WBCP. Eosinophils were higher (P \u3c 0.05) in CPS (333.33) compared to PPS birds (148.00) at week 5. The HLR was lower (P \u3c 0.05) in PPS broilers at week 5 (0.40) than weeks 3 (0.44) and 7 (0.43). The PPS broilers experienced less stress overall than CPS birds. Keywords: Conventional Production System, Broilers, Pasture Production System, Stres

Evaluating the Economic Feasibility of Producing Broilers on Pasture and in a Conventional Production System

The demand for pasture raised poultry is increasing because of the perception that it is healthier and tastier than conventionally raised poultry. In order to meet this demand, some producers are considering switching from the conventional to the pasture system to take advantage of this niche market. Therefore, the objective of the study was to determine the economic viability of rearing broilers in a pastured poultry system (PPS) versus a conventional poultry system (CPS). Each of these systems was replicated three times with 60 birds per treatment in a study lasting seven weeks. Data were collected on costs of inputs, and others estimated. The mean net returns for PPS and CPS were, respectively, $56.44 and -$92.02. The break-even prices were, respectively, $10.89 for PPS and$11.87 for CPS. Also, the benefit/cost ratio was 1.103 for PPS and 0.845 for CPS. The paired t-test for the benefit/cost ratios indicated a significant difference (p< 0.05) between PPS and CPS. PPS was deemed more feasible than CPS. Hence, it was recommended that the former system is more appropriate for small and limited resource producers, and that technical assistance should be provided to them regarding PPS. Keywords: Pastured poultry, Conventional poultry, Economic feasibility, Broiler

Heliophysics and Amateur Radio:Citizen Science Collaborations for Atmospheric, Ionospheric, and Space Physics Research and Operations

The amateur radio community is a global, highly engaged, and technical community with an intense interest in space weather, its underlying physics, and how it impacts radio communications. The large-scale observational capabilities of distributed instrumentation fielded by amateur radio operators and radio science enthusiasts offers a tremendous opportunity to advance the fields of heliophysics, radio science, and space weather. Well-established amateur radio networks like the RBN, WSPRNet, and PSKReporter already provide rich, ever-growing, long-term data of bottomside ionospheric observations. Up-and-coming purpose-built citizen science networks, and their associated novel instruments, offer opportunities for citizen scientists, professional researchers, and industry to field networks for specific science questions and operational needs. Here, we discuss the scientific and technical capabilities of the global amateur radio community, review methods of collaboration between the amateur radio and professional scientific community, and review recent peer-reviewed studies that have made use of amateur radio data and methods. Finally, we present recommendations submitted to the U.S. National Academy of Science Decadal Survey for Solar and Space Physics (Heliophysics) 2024–2033 for using amateur radio to further advance heliophysics and for fostering deeper collaborations between the professional science and amateur radio communities. Technical recommendations include increasing support for distributed instrumentation fielded by amateur radio operators and citizen scientists, developing novel transmissions of RF signals that can be used in citizen science experiments, developing new amateur radio modes that simultaneously allow for communications and ionospheric sounding, and formally incorporating the amateur radio community and its observational assets into the Space Weather R2O2R framework. Collaborative recommendations include allocating resources for amateur radio citizen science research projects and activities, developing amateur radio research and educational activities in collaboration with leading organizations within the amateur radio community, facilitating communication and collegiality between professional researchers and amateurs, ensuring that proposed projects are of a mutual benefit to both the professional research and amateur radio communities, and working towards diverse, equitable, and inclusive communities

COMPARING THE EFFECTS OF CONVENTIONAL AND PASTURED POULTRY PRODUCTION SYSTEMS ON THE STRESS LEVELS OF BROILERS

The objective of this study was to measure stress levels in broilers raised on a pasture production system (PPS) and a conventional production system (CPS) by evaluating lymphoid organ weights, white blood cell profiles (WBCP), total white blood cell counts (TWBC), and heterophil to lymphocyte ratios (HLR). Broilers were brooded indoors for 3 weeks then divided into 2 groups; one placed on pasture while the other remained indoors. Blood was collected at weeks 3, 5, and 7 via brachial venipuncture. After 49 days, birds were slaughtered and lymphoid organs harvested. Results showed no differences between treatments for lymphoid organs, TWBC, or WBCP. Eosinophils were higher (P < 0.05) in CPS (333.33) compared to PPS birds (148.00) at week 5. The HLR was lower (P < 0.05) in PPS broilers at week 5 (0.40) than weeks 3 (0.44) and 7 (0.43). The PPS broilers experienced less stress overall than CPS birds

Soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) Is Elevated in Bronchoalveolar Lavage Fluid of Patients with Acute Respiratory Distress Syndrome.

INTRODUCTION:Pulmonary vascular endothelial activation has been implicated in acute respiratory distress syndrome (ARDS), yet little is known about the presence and role of endothelial activation markers in the alveolar space in ARDS. We hypothesized that endothelial activation biomarkers would be differentially expressed in bronchoalveolar lavage fluid from patients with ARDS compared with healthy volunteers, and that biomarker concentrations would be associated with ARDS severity. METHODS:We performed a cross-sectional analysis of data from 26 intubated patients with ARDS undergoing evaluation for clinically suspected ventilator-associated pneumonia and five healthy volunteers. Patients underwent bronchoalveolar lavage a median of five days after intubation. Healthy volunteers also underwent bronchoalveolar lavage. Endothelial activation biomarkers (soluble vascular cell adhesion molecule-1 [sVCAM-1], soluble endothelial selectin [sESEL], angiopoietin-1 [Ang-1] and angiopoietin-2 [Ang-2]) were measured in bronchoalveolar lavage fluid. Clinically suspected ventilator-associated pneumonia was confirmed with microbiologic culture data. RESULTS:Patients with ARDS had significantly higher median sVCAM-1 concentrations in the bronchoalveolar lavage fluid compared with healthy volunteers (985 vs 119 pg/mL, p = 0.03). Additionally, there was a trend toward greater bronchoalveolar lavage fluid sVCAM-1 concentrations among patients with moderate/severe compared to mild ARDS (1395 vs 209 pg/mL, p = 0.06). We did not detect significant differences in bronchoalveolar lavage fluid levels of sESEL, Ang-1 or Ang-2 between patients with ARDS and healthy volunteers. Median bronchoalveolar lavage fluid biomarker levels did not differ between patients with and without microbiologically-confirmed ventilator-associated pneumonia. CONCLUSIONS:sVCAM-1 concentrations were significantly higher in the bronchoalveolar lavage fluid of patients with ARDS compared to healthy controls, and tended to be higher in moderate/severe ARDS compared to mild ARDS. Our findings add to the growing evidence supporting the concept that endothelial activation plays an important mechanistic role in the pathogenesis of ARDS. Further studies are necessary to characterize the role and/or clinical significance of sVCAM-1 and other endothelial activation markers present in the alveolar space in ARDS

Levels of endothelial activation biomarkers in bronchoalveolar lavage fluid, by ARDS severity and microbiologically-confirmed ventilator-associated pneumonia.

<p><u><b>Panel A</b></u>: Bronchalveolar lavage fluid (BALF) biomarker levels stratified by presence and severity of acute respiratory distress syndrome (ARDS) (**<i>Control vs</i>. <i>ARDS</i>, <i>p = 0</i>.<i>03</i>, <i>Mild vs</i>. <i>Moderate/Severe ARDS p = 0</i>.<i>06</i>). <u><b>Panel B</b></u>: BALF biomarker levels stratified by presence of microbiologically-confirmed ventilator-associated pneumonia (VAP).</p

Levels of endothelial activation biomarkers (pg/mL) in bronchoalveolar lavage fluid of ARDS patients and controls.

<p>Levels of endothelial activation biomarkers (pg/mL) in bronchoalveolar lavage fluid of ARDS patients and controls.</p

Levels of endothelial activation biomarkers (pg/mL) in bronchoalveolar lavage fluid restricted to patients <i>without</i> microbiologically-confirmed VAP and controls.

<p>Levels of endothelial activation biomarkers (pg/mL) in bronchoalveolar lavage fluid restricted to patients <i>without</i> microbiologically-confirmed VAP and controls.</p

Baseline characteristics of ARDS patients stratified by ARDS severity.

<p>Baseline characteristics of ARDS patients stratified by ARDS severity.</p