87 research outputs found
A makro-TSH diagnosztikus és terápiás jelentősége Hashimoto-thyreoiditises betegekben | Diagnostic and therapeutical significance of macro-TSH in patients with Hashimoto’s thyroiditis
Absztrakt:
Bevezetés: A makro-TSH szerkezete, incidenciája és klinikai
szerepe pajzsmirigybetegekben nem tisztázott. Célkitűzés: A
makro-TSH előfordulási gyakoriságának, tulajdonságainak meghatározása
Hashimoto-thyreoiditises betegek savójában. Módszer: A
Hashimoto-thyreoiditises betegek vérmintáiban a makro-TSH-t meghatározták
polietilén-glikol precipitációs módszerrel és protein G agaróz abszorpciós,
illetve gélfiltrációs kromatográfiával. A makro-TSH biológiai aktivitását
TSH-receptorral transzfektált CHO bioassay módszerével mérték. A betegek
L-tiroxin-kezelésben részesültek (átlagosan 66,5 µg/nap), a betegek fele pedig
szelént is kapott (átlagosan 60 µg/nap). Eredmények: 880
Hashimoto-thyreoiditises beteget (728 nő, átlagéletkor 44,8 év) vontak be a
vizsgálatba. A makro-TSH-t 41 betegben (4,6%) mutatták ki, az átlagos TSH-szint
a PEG-precipitáció előtt 185,4 ± 35 IU/l volt, a precipitáció után pedig 5,55 ±
1,8 IU/l. Az anti-TPO-szint 445 ± 51 IU/l volt és fokozatosan csökkent 212 ± 51
IU/l-re egyéves tiroxin- és szelénkezelés után. Mind a PEG-precipitációs, mind a
protein G abszorpciós módszerrel, illetve gélkromatográfiás eljárással a TSH
elleni antitest jelenlétét mutatták ki a makro-TSH-immunkomplexben. A makro-TSH
biolĂłgialag inaktĂvnak bizonyult, mivel a CHO-sejteket nem stimulálta. A
makro-TSH a szelénnel nem kezelt csoportban 18 ± 3,2 hónapig, a szelénnel
kezeltben 12 ± 1,9 hónapig volt kimutatható. Következtetés: A
TSH elleni antitestek fő komponensei a makro-TSH-nak és diagnosztikus, illetve
terápiás nehézségeket okozhatnak. A PEG-precipitációs eljárás alkalmas
szűrĹ‘mĂłdszer a makro-TSH bizonyĂtására. A szelĂ©n kĂ©pes nemcsak az anti-TPO-,
hanem a makro-TSH-szint csökkentésére egyaránt. Amikor a TSH-szint 40,0 IU/l
feletti a hypothyreosis jelei nélkül, gondolnunk kell a makro-TSH jelenlétére.
Orv Hetil. 2017; 158(34): 1346–1350.
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Abstract:
Introduction: Structure, importance and incidence and clinical
role of macro-TSH not clarified in thyroid diseases. Aim: This
study was undertaken to determine the incidence and biological role of macro-TSH
in patients with Hashimoto’s thyroiditis. Method: Blood samples
taken from patients with Hashimoto’s thyroiditis were screened for the presence
of macro-TSH with the polyethylene glycol method and confirmed with protein G
agarose absorption test and gel filtration chromatography. Stimulatory capacity
of macro-TSH was measured by CHO cells bio-assay. Patients were treated with
L-thyroxine (mean 66.5 µg/day) and half of them with selenium (mean 60 µg/day),
respectively. Results: 880 patients (728 female, aged 44.8
yr) with Hashimoto’s thyroiditis was involved in the study. Macro-TSH was found
in the serum of 41 patients (4.6%), the mean TSH 185.4 ± 35 IU/l was before PEG
precipitations and after 5.55 ± 1.8 IU/l. Titre of anti-TPO proved to be 445 ±
51 IU/l and gradulally decreased to 212 ± 51 IU/l after one year therapy. Both
the precipitation, protein G absorption and gel chromatography supported the
presence of anti-TSH antibody in the macro-TSH complex. Stimulatory capacity of
macro-TSH on CHO bio-assay was not proved. The macro-TSH was detected in the
selenium not treated group for 18 ± 3.2 months, selenium-treated for 12 ± 1.9
months. Conclusion: It is concluded that anti-human TSH
autoantibodies are a major components of macro-TSH and may cause diagnostic and
therapeutical difficulties. The PEG precipitation is a suitable screening method
for detection of macro-TSH. Selenium is able to decrease of anti-TPO antibodies
and macro-TSH, respectively. When the TSH level is greater than 40.0 IU/l,
without the signs of hypothyroidism, the presence of macro-TSH is to be
considered. Orv Hetil. 2017; 158(34): 1346–1350
Model Curves of Root Tensile Resistance with Root Diameter for All Moisture Content Stages.
<p><i>D</i>: Diameter/mm.</p
Effect of Root Moisture Content and Diameter on Root Tensile Properties
<div><p>The stabilization of slopes by vegetation has been a topical issue for many years. Root mechanical characteristics significantly influence soil reinforcement; therefore it is necessary to research into the indicators of root tensile properties. In this study, we explored the influence of root moisture content on tensile resistance and strength with different root diameters and for different tree species. <i>Betula platyphylla</i>, <i>Quercus mongolica</i>, <i>Pinus tabulaeformis</i>, and <i>Larix gmelinii</i>, the most popular tree species used for slope stabilization in the rocky mountainous areas of northern China, were used in this study. A tensile test was conducted after root samples were grouped by diameter and moisture content. The results showedthat:1) root moisture content had a significant influence on tensile properties; 2) slightly loss of root moisture content could enhance tensile strength, but too much loss of water resulted in weaker capacity for root elongation, and consequently reduced tensile strength; 3) root diameter had a strong positive correlation with tensile resistance; and4) the roots of <i>Betula platyphylla</i> had the best tensile properties when both diameter and moisture content being controlled. These findings improve our understanding of root tensile properties with root size and moisture, and could be useful for slope stabilization using vegetation.</p></div
Photo of the universal tester used for tensile test.
<p>Photo of the universal tester used for tensile test.</p
Four Examples of Stress-Strain Curves.
<p><i>Quercus mongolica D2H1</i>: one <i>Quercus mongolica</i>sample with diameter in <i>D2</i> class and moisture content in <i>H1</i>stage; <i>Pinus tabulaeformis D3H1</i>: one <i>Pinus tabulaeformis</i> sample with diameter in <i>D3</i> class and moisture content in <i>H1</i> stage; <i>Quercus mongolica D3H6</i>: one <i>Quercus mongolica</i> sample with diameter in <i>D3</i> class and moisture content in <i>H6</i>stage; <i>Pinus tabulaeformis D4H6</i>: one <i>Pinus tabulaeformis</i> sample with diameter in <i>D4</i> class and moisture content in <i>H6</i>stage.</p
Trend Graphs of Root Tensile Strength with Moisture Content for All Diameter Classes.
<p><i>H</i>: Root moisture content stage.</p
Summary of sample’s physical features and statistics.
<p>Summary of sample’s physical features and statistics.</p
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