Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro

The adjuvant chemotherapy, such as cisplatin, doxorubicin, and methotrexate has significantly improved survival of osteosarcoma patients. However, the chemoresistance which arose with the chemotherapy blocks achieving favorable outcomes for some patients and finally led to relapse or metastatic disease. Studies have shown paradoxical functions of autophagy in tumor development, which has been demonstrated by microRNAs. In the present study, we determined the involvement of autophagy during the chemotherapy of osteosarcoma cell line, U-2 OS, and further determined the regulation of miR-101 on the autophagy in the U-2 OS cells. Results demonstrated that doxorubicin treatment of U-2 OS cells induced significantly high level of autophagy-characteristic acidic vesicular organelles (AVOs), and induced significant autophagy related protein expression in U-2 OS cells. While the miR-101 could significantly reduce the doxorubicin-induced AVOs and block the autophagy related protein expression in U-2 OS cells. Moreover, the autophagy blockage by miR-101 sensitized the U-2 OS cells to doxorubicin treatment. In summary, miR-101 blocks autophagy during the chemotherapy in osteosarcoma cells and enhances chemosensitivity in vitro

Dry Sliding Tribological Behaviors of Electrodeposited Ni-GO/SiC Composite Coating on the 2218 Aluminum Alloy

Electrodeposition has attracted tremendous interest in functional coatings due to its advantages of high efficiency, inexpensiveness and ease of implementation. In this work, nickel graphene oxide (Ni-GO), nickel silicon carbide (Ni-SiC) and nickel graphene oxide/silicon carbide (Ni-GO/SiC) composite coatings were electrodeposited on the 2218 aluminum alloy (2218AlA) substrate. The microstructure, microhardness, bonding strength and tribological behaviors of the composite coatings were carried out. According to the results obtained, the composite coatings were dense and compact, with no visible defects and microcracks, and well bonded to 2218AlA substrate. The microhardness of composite coatings was significantly increased compared to that of the 2218AlA substrate. The microhardness of Ni-SiC composite coating was the highest, reaching 3.14 times that of the 2218AlA substrate. The friction response time, friction coefficient and wear rate of the composite coatings were obviously lower. For the Ni-GO composite coating, the average friction coefficient is the smallest at 45.35% of the 2218AlA substrate, while the wear rate is the smallest at 46.97% of the 2218AlA substrate. However, the comprehensive tribological performances of the Ni-GO/SiC composite coating were superior. The abrasive and adhesive wear were the main wear mechanisms of composite coatings, but the degree of damage was different

Dry Sliding Tribological Behaviors of Electrodeposited Ni-GO/SiC Composite Coating on the 2218 Aluminum Alloy

Electrodeposition has attracted tremendous interest in functional coatings due to its advantages of high efficiency, inexpensiveness and ease of implementation. In this work, nickel graphene oxide (Ni-GO), nickel silicon carbide (Ni-SiC) and nickel graphene oxide/silicon carbide (Ni-GO/SiC) composite coatings were electrodeposited on the 2218 aluminum alloy (2218AlA) substrate. The microstructure, microhardness, bonding strength and tribological behaviors of the composite coatings were carried out. According to the results obtained, the composite coatings were dense and compact, with no visible defects and microcracks, and well bonded to 2218AlA substrate. The microhardness of composite coatings was significantly increased compared to that of the 2218AlA substrate. The microhardness of Ni-SiC composite coating was the highest, reaching 3.14 times that of the 2218AlA substrate. The friction response time, friction coefficient and wear rate of the composite coatings were obviously lower. For the Ni-GO composite coating, the average friction coefficient is the smallest at 45.35% of the 2218AlA substrate, while the wear rate is the smallest at 46.97% of the 2218AlA substrate. However, the comprehensive tribological performances of the Ni-GO/SiC composite coating were superior. The abrasive and adhesive wear were the main wear mechanisms of composite coatings, but the degree of damage was different

HIF-1α had Pivotal Effects on Downregulation of miR-210 Decreasing Viability and Inducing Apoptosis in Hypoxic Chondrocytes

Hypoxia-inducible factor 1-alpha (HIF-1α) and some microRNA (miRNAs) play pivotal roles in response to hypoxia-related physiologic and pathophysiologic responses. Up to date, the regulatory mechanisms of these molecules were largely unknown in chondrocytes. In this study, to study the mechanisms of degradation and homeostasis of chondrocytes, the effects of miRNAs and HIF-1α on chondrocytes in physiologic environment were investigated. We found that the overexpression of miR-210 and HIF-1α was present on hypoxia in C28/I2 human chondrocytes significantly by qRT-PCR and western plot. Further study displayed that miR-210 played positive role as a promoter in regulation and its regulated molecules (bcl-xl and PHD-2) in C28/I2 cells on hypoxia by silenced miR-210, silenced HIF-1α, and adding miR-210. Moreover, downregulated miR-210 could significantly repress the viability and increase the apoptosis in C28/I2 cells on hypoxia, compared to those on normoxia. Furthermore, miR-210 could not modulate viability and apoptosis in C28/I2 cells with the HIF-1α knockdown on hypoxia and normoxia. Taken together, this study demonstrated that the MiR-210 was involved in an HIF-1α-dependent way in C28/I2 human chondrocytes for the first time. It also suggested that miR-210 downregulation decreased viability and induced apoptosis in hypoxic chondrocytes depending on HIF-1α

Characterization and Wear Behaviors of Electrodeposited Ni-MoS2/SiC Composite Coating

Among the preparation methods of functional coatings, the electrodeposition technique has attracted much attention due to its advantages of economy, high efficiency and good structural adaptability. The application of aluminum alloy materials is greatly limited due to their poor friction reduction and wear resistance. Therefore, to enhance the tribological behaviors of aluminum alloy materials, the Ni-MoS2, Ni-SiC and Ni-MoS2/SiC composite coatings were prepared on the 2218 aluminum alloy by an electrodeposition technique. The prepared composite coating samples exhibited a compact and dense microstructure, which was consistent with the result of their high microhardness. No obvious microcracks and defects appeared at the interfaces, indicating that the composite coating samples had good adhesion to the substrates and can effectively improve the frictional shear resistance. The results of wear experiment showed that the wear rate, friction coefficient and friction response time of all composite coating samples were lower than that of the substrate sample. However, the friction reduction and wear resistance of the same composite coating sample were not consistent. The friction coefficient of the Ni-MoS2 composite coating sample was the lowest, and the wear rate of the Ni-SiC composite coating sample was the lowest. According to the worn surface observations, the wear mechanism of composite coating samples was mainly characterized by the mild abrasive wear, flake spalling, tearing and pits caused by particle shedding, and the substrate sample showed a severe adhesive wear and abrasive wear

The Ionic Organic Cage: An Effective and Recyclable Testbed for Catalytic CO2 Transformation

Porous organic cages (POC) are a class of relatively new molecular porous materials, whose concept was raised in 2009 by Cooper’s group and has rarely been directly used in the area of organic catalysis. In this contribution, a novel ionic quasi-porous organic cage (denoted as Iq-POC), a quaternary phosphonium salt, was easily synthesized through dynamic covalent chemistry and a subsequent nucleophilic addition reaction. Iq-POC was applied as an effective nucleophilic catalyst for the cycloaddition reaction of CO2 and epoxides. Owing to the combined effect of the relatively large molecular weight (compared with PPh3+I−) and the strong polarity of Iq-POC, the molecular catalyst Iq-POC displayed favorable heterogeneous nature (i.e., insolubility) in this catalytic system. Therefore, the Iq-POC catalyst could be easily separated and recycled by simple centrifugation method, and the catalyst could be reused five times without obvious loss of activity. The molecular weight augmentation route in this study (from PPh3+I− to Iq-POC) provided us a “cage strategy” of designing separable and recyclable molecular catalysts

Infertility-Related Stress and Life Satisfaction among Chinese Infertile Women: A Moderated Mediation Model of Marital Satisfaction and Resilience

The present study investigated the effects of infertility on Chinese women&#39;s life satisfaction. Infertile women (n = 466) who were seeking medical help completed a survey that included the Fertility Problem Inventory (FPI), the Dyadic Adjustment Questionnaire (DAS), the Connor-Davidson Resilience Scale (CD-RISC), the Satisfaction with Life Scale, and demographic variables. We used a moderated mediation model to test our hypotheses, with life satisfaction as the dependent variable, representations about the importance of parenthood as the independent variable, the impact of infertility on life domains as a mediator, and marital satisfaction and resilience as moderators. Results showed that representations about the importance of parenthood and the impact of infertility on life domains are two main types of infertility-related stress, which could play independent roles in predicting life satisfaction. Representations about the importance of parenthood had a negative indirect effect on life satisfaction through the impact of infertility on life domains, and the indirect effect of the impact of infertility on life domains was moderated by marital satisfaction and resilience. Specially, representations about the importance of parenthood had a weaker indirect effect (through the impact of infertility on life domains) on life satisfaction in individuals with higher marital satisfaction or resilience. Therefore, the type of infertility-related stress and both marital satisfaction and resilience should be addressed in psychological interventions for women coping with infertility in mainland China.</p

The Displacement Analysis and Prediction of a Creeping Ancient Landslide at Suoertou, Zhouqu County, China

The ancient Suoertou landslide seriously threatens the surrounding population&#x0027;s lives and property. Monitoring and predicting this landslide is crucial to ensure the affected areas&#x2019; safety. The previous research on the landslide&#x0027;s displacement characteristics and mechanisms has lacked detailed analyses. In addition, its future development trends must be understood. Therefore, we conducted a detailed analysis of the ancient Suoertou landslide&#x0027;s displacement characteristics and mechanisms using small baseline subset interferometric synthetic aperture radar monitoring results from 2018 to 2023. Furthermore, by applying a gated recurrent unit prediction mode that incorporates the refined displacement characteristics and mechanisms, we forecasted the landslide&#x0027;s displacement trends. The results show that this landslide is currently undergoing overall slow displacement with violent fluctuations in localized areas. Tectonic movement, precipitation, human activities, river erosion, and other factors interact, forming a vicious development displacement mechanism. According to our prediction, the displacement of this landslide will be in a trend of fluctuating increase from June 2023 to June 2024. In particular, the local area will undergo abnormal displacement acceleration. The results of the current research provide a scientific basis upon which to monitor landslides, promote their management, and reduce the risk of losses due to landslide disasters

Effects of Fructus Psoraleae Extract on the Intestinal Absorption Kinetics of Geniposide and Geniposidic Acid in Rat

Cortex Eucommia has been used as a kidney-tonifying herbal medicine with a long history of compatibility with Fructus Psoraleae. Geniposide (GP) and geniposidic acid (GPA) are the two main chemical components in Cortex Eucommia. In the present study, the effects of Fructus Psoraleae extract (FPE) on intestinal absorption kinetics of GP and GPA in rat were investigated. Twenty four male Sprague-Dawley rats were randomly assigned into four groups which were treated with GP, GPA, GP mixed with FPE and GPA mixed with FPE, respectively, by in situ intestinal perfusion for 3 h. The samples of intestinal perfusion solutions were collected every 30 min, and analyzed by ultra high performance liquid chromatography (UPLC). The curves of time and residual quantities of GP and GPA (lnx) in the intestinal perfusion solution and the cumulative absorption rate were obtained. The results showed that FPE exhibited different effects on the intestinal absorption of GP and GPA in rat: it increased the intestinal absorption of GP (p &lt; 0.05), while demonstrated no significant effect on the absorption of GPA