Application of Biophysical Techniques to Cellular and Molecular Oncology.

Dysregulated cellular processes drive malignant transformation, tumor progression, and metastasis, and affect responses to therapies [...]

Mast cell synapses and exosomes: membrane contacts for information exchange.

Contains fulltext : 108620.pdf (publisher's version ) (Open Access)In addition to their central role in allergy, mast cells are involved in a wide variety of cellular interactions during homeostasis and disease. In this review, we discuss the ability of mast cells to extend their mechanisms for intercellular communication beyond the release of soluble mediators. These include formation of mast cell synapses on antigen presenting surfaces, as well as cell-cell contacts with dendritic cells and T cells. Release of membrane bound exosomes also provide for the transfer of antigen, mast cell proteins, and RNA to other leukocytes. With the recognition of the extended role mast cells have during immune modulation, further investigation of the processes in which mast cells are involved is necessary. This reopens mast cell research to exciting possibilities, demonstrating it to be an immunological frontier

Visualizing signaling nanoplatforms at a higher spatiotemporal resolution

The International Symposium entitled ‘Visualizing signaling nanoplatforms at a higher spatiotemporal resolution’ sponsored by the Institució Catalana de Recerca i Estudis Avançats (ICREA) was held on 29–31 May 2013 at the ICFO-Institute of Photonic Sciences, in Barcelona, Spain. The meeting brought together a multidisciplinary group of international leaders in the fields of super-resolution imaging (nanoscopy) and cell membrane biology, and served as a forum to further our understanding of the fundamental mechanisms that govern nanostructures and protein–function relationships at the cell membran

Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)\ud

We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular proteins in vivo. They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases involved in growth-factor-mediated signal transduction.\ud \u

Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells.

Contains fulltext : 52013.pdf (publisher's version ) (Closed access)The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo

Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation

Mast cells (MCs) produce soluble mediators such as histamine and prostaglandins that are known to influence dendritic cell (DC) function by stimulating maturation and antigen processing. Whether direct cell-cell interactions are important in modulating MC/DC function is unclear. In this paper, we show that direct contact between MCs and DCs occurs and plays an important role in modulating the immune response. Activation of MCs through FcepsilonRI cross-linking triggers the formation of stable cell-cell interactions with immature DCs that are reminiscent of the immunological synapse. Direct cellular contact differentially regulates the secreted cytokine profile, indicating that MC modulation of DC populations is influenced by the nature of their interaction. Synapse formation requires integrin engagement and facilitates the transfer of internalized MC-specific antigen from MCs to DCs. The transferred material is ultimately processed and presented by DCs and can activate T cells. The physiological outcomes of the MC-DC synapse suggest a new role for intercellular crosstalk in defining the immune response