Soft tissue grafts for dural reconstruction after meningioma surgery

The meninges are involved in various pathologies and are often directly or indirectly severed during surgical procedures, especially the dura mater. This can pose a real challenge for the surgeon, as a proper reconstruction of the meninges is important to prevent complications such as cerebrospinal fluid leak (CSF). A variety of techniques for dural reconstruction have been described, employing natural and artificial materials. A novel technique for dural reconstruction involves soft tissue grafts in the form of fibrous or fibromuscular flaps, which are placed on the dural defects to seal the gaps. These soft tissue grafts represent an appropriate scaffold for cell ingrowth and fibrosis, thus preventing CSF. In this pilot study, we described the application of soft tissue grafts for dural reconstruction in 10 patients who underwent convexity meningioma surgery

A novel polymerase chain reaction (PCR) based assay for authentication of cell lines or tissues from human, pig and chicken origin

A polymerase chain reaction based assay was developed for authentication of cell lines or tissues from human, pig and chicken origin. Specificity was achieved by species specific primer design targeting the mitochondrial D-loop sequence. Amplicon sizes were 114 bp, 169 bp and 645-648 bp for chicken, human and pig derived cell lines, respectively. Primers were tested for species specificity and non-specificity between haplogroups of the same organisms using BLAST tool and subsequently for cross amplification DNA extracted from human, chicken and pig venous blood as a positive control. Primers were also amplifying specific products in DNA extracted from individual cell line in both functional cell models and intentionally mixed cell lines consisting functional cell models. The PCR assay developed in this study represents a low-cost species specific end-point PCR based assay of the mitochondrial D-loop for the authentication of the cell line origin

Clinical Applications of Poly-Methyl-Methacrylate in Neurosurgery: The In Vivo Cranial Bone Reconstruction

Background: Biomaterials and biotechnology are becoming increasingly important fields in modern medicine. For cranial bone defects of various aetiologies, artificial materials, such as poly-methyl-methacrylate, are often used. We report our clinical experience with poly-methyl-methacrylate for a novel in vivo bone defect closure and artificial bone flap development in various neurosurgical operations. Methods: The experimental study included 12 patients at a single centre in 2018. They presented with cranial bone defects after various neurosurgical procedures, including tumour, traumatic brain injury and vascular pathologies. The patients underwent an in vivo bone reconstruction from poly-methyl-methacrylate, which was performed immediately after the tumour removal in the tumour group, whereas the trauma and vascular patients required a second surgery for cranial bone reconstruction due to the bone decompression. The artificial bone flap was modelled in vivo just before the skin closure. Clinical and surgical data were reviewed. Results: All patients had significant bony destruction or unusable bone flap. The tumour group included five patients with meningiomas destruction and the trauma group comprised four patients, all with severe traumatic brain injury. In the vascular group, there were three patients. The average modelling time for the artificial flap modelling was approximately 10 min. The convenient location of the bone defect enabled a relatively straightforward and fast reconstruction procedure. No deformations of flaps or other complications were encountered, except in one patient, who suffered a postoperative infection. Conclusions: Poly-methyl-methacrylate can be used as a suitable material to deliver good cranioplasty cosmesis. It offers an optimal dural covering and brain protection and allows fast intraoperative reconstruction with excellent cosmetic effect during the one-stage procedure. The observations of our study support the use of poly-methyl-methacrylate for the ad hoc reconstruction of cranial bone defects

A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation.

The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are

Novel and established intestinal cell line models - An indispensable tool in food science and nutrition

The aim of this review is to present the recent developments and examine the future potential of functional cell models as indispensable in vitro tools in current food and nutritional science fields. Cell models provide the platform for preliminary research and contribute in the substantiation of health claims in foods by elucidating the biological plausibility through the involved mechanism of action. Applications include research on bioavailability and adsorption/transport in nutritional or toxicological settings, studies on the allergic effects of food components, host-pathogen gut interactions as well as risk assessment and modelling of potential hazardous substances in the food chain.JRC.I.5-Systems Toxicolog

MTT assay.

<p>Three independent tests were performed and the results were expressed by the mean ratios (%, ± SD) of absorbance in treated wells to those in control wells. ANOVA, *p<0.001, **p<0.05 compared to control.</p

NF-kB p65 and STAT1 translocations in TLT macrophages.

<p>a) Percentage of TLT macrophage cells in basolateral co-culture with H4-1 cells in which translocation of STAT1 and NF-kB occurred after treatment with different bacterial strains and positive controls. b) Acquired images of H4-1 cells with cytoplasmic or translocating probes. * indicates significant differences from control cells (p<0.05).</p

Myoblast proliferation rate.

<p>The figure shows the proliferation of myoblasts after 7 days of incubation with DMEM supplemented with decorin 10 ng/ml, decorin 25 ng/ml, 10% PRP exudate and 20% PRP exudate. Three independent tests were performed and the results are expressed by the absolute absorbance values (in blue) and mean ratios (%, ± SD) of absorbance in treated wells to those in control wells (in grey). ANOVA, *p<0.001 compared with control.</p

NF-kB p65 and STAT1 translocations in small intestinal epithelial cells.

<p>a) Percentage of H4-1 small intestinal epithelial cells in co-culture with TLT macrophages in which translocation of STAT1 and NF-kB occurred after treatment with different bacterial strains and positive controls. b) Acquired images of H4-1 cells with cytoplasmic or translocating probes. * indicates significant differences from control cells (p<0.05).</p

TGF-β and MSTN expression (ELISA).

<p>Three independent tests were performed for each cytokine and the results are expressed by the mean ratios (%) of absorbance in treated wells to those in control wells. ANOVA, p<0.001 compared to control.</p