Repetibilidade dos parâmetros de adaptabilidade e estabilidade do feijoeiro em ambientes imprevisíveis

The objective of this work was to estimate the repeatability of adaptability and stability parameters of common bean between years, within each biennium from 2003 to 2012, in Minas Gerais state, Brazil. Grain yield data from trials of value for cultivation and use common bean were analyzed. Grain yield, ecovalence, regression coefficient, and coefficient of determination were estimated considering location and sowing season per year, within each biennium. Subsequently, a analysis of variance these estimates was carried out, and repeatability was estimated in the biennia. Repeatability estimate for grain yield in most of the biennia was relatively high, but for ecovalence and regression coefficient it was null or of small magnitude, which indicates that confidence on identification of common bean lines for recommendation is greater when using means of yield, instead of stability parameters.O objetivo deste trabalho foi estimar a repetibilidade dos parâmetros de adaptabilidade e estabilidade do feijoeiro entre anos, dentro de cada biênio de 2003 a 2012, em Minas Gerais, Brasil. Os dados de produtividade de grãos provenientes de ensaios de valor de cultivo e uso de feijoeiro‑comum foram analisados. A produtividade de grãos, a ecovalência, o coeficiente de regressão e o coeficiente de determinação foram estimados quanto a local e época de semeadura por ano, dentro de cada biênio. Posteriormente, realizou-se análise de variância destas estimativas, e a repetibilidade foi estimada nos biênios. A estimativa de repetibilidade quanto à produtividade de grãos, na maioria dos biênios, foi relativamente alta, mas, quanto à ecovalência, ao coeficiente de determinação e ao coeficiente de regressão, foi nula ou de pequena magnitude, o que indica maior confiança na identificação de linhagens de feijão a serem recomendadas, quando se usam as medidas de produtividade, em vez dos parâmetros de estabilidade

Selection of common bean inbred lines with tolerance to high moisture at harvest Seleção de linhagens de feijão com tolerância a alta umidade no momento da colheita

The occurrence of precipitation / rain in harvest bean causes damage to the product and reduces the yield. The main alternative to mitigate this problem is to obtain cultivars presenting low germination of beans in the pods under conditions of high moisture. The purpose of this study was to verify if there is genetic variability among the common bean inbred lines that are in the phase of recommendation for the South of Minas Gerais, Brazil in regard to tolerance to moisture after harvest, and identify traits that may be routinely used in selection of tolerant lines to these conditions. Ninety-five lines in the phase of recommendation by the Genetic Breeding Program from UFLA were used. After harvest, a sample of plants from each plot was removed for assessments of seed germination in the pods in a moist chamber and water absorption by pod and seed. Eight days after harvest, another sample was removed to assess seed appearance using a scoring scale. The data were submitted to analysis of variance and estimates of the Pearson phenotypic correlations were obtained among traits. Lines differ in relation to tolerance to moisture at the time of harvest, with those of higher tolerance having lighter colored seeds. The main difficulty in selection of common bean lines for tolerance to high moisture at harvest is the repeatability of the environmental conditions among crop seasons. The alternative is assessing the amount of water absorbed by pods.<br>A ocorrência de precipitação / chuva na colheita do feijão comum provoca danos ao produto e reduz o rendimento. A principal alternativa para mitigar este problema é a obtenção de cultivares com baixa germinação de grãos nas vagens em condições de alta umidade. Neste trabalho, objetivou-se vrificar se há variabilidade genética entre as linhagens do feijoeiro que estão em fase de recomendação para o Sul de Minas Gerais quanto à tolerância à umidade após a colheita e identificar caracteres que possam ser utilizados rotineiramente na seleção de linhagens tolerantes a essas condições. Utilizaram-se 95 linhagens em fase de recomendação pelo Programa de Melhoramento Genético da UFLA. Após a colheita, uma amostra de plantas de cada parcela foi retirada para avaliações de germinação dos grãos nas vagens em câmara de nebulização e absorção de água pela vagem e pelo grão. Oito dias após o arranquio foi retirada outra amostra para avaliar o aspecto do grão utilizando escala de notas. Os dados foram submetidos à análise de variância e obtidas as estimativas das correlações fenotípicas de Pearson entre os caracteres dois a dois. As linhagens diferem com relação à tolerância à umidade no momento da colheita, sendo que as de maior tolerância apresentam grãos mais claros. A principal dificuldade na seleção de linhagens de feijoeiro comum para a tolerância a alta umidade na colheita é a repetibilidade das condições ambientais entre as safras. A alternativa é avaliar a quantidade de água absorvida pelas vagens

Repeatability of adaptability and stability parameters of common bean in unpredictable environments

The objective of this work was to estimate the repeatability of adaptability and stability parameters of common bean between years, within each biennium from 2003 to 2012, in Minas Gerais state, Brazil. Grain yield data from trials of value for cultivation and use common bean were analyzed. Grain yield, ecovalence, regression coefficient, and coefficient of determination were estimated considering location and sowing season per year, within each biennium. Subsequently, a analysis of variance these estimates was carried out, and repeatability was estimated in the biennia. Repeatability estimate for grain yield in most of the biennia was relatively high, but for ecovalence and regression coefficient it was null or of small magnitude, which indicates that confidence on identification of common bean lines for recommendation is greater when using means of yield, instead of stability parameters

Implications of predictable and unpredictable environmental factors in common bean VCU trials in Minas Gerais

The aim of this study was to estimate the relative contribution of predictable and unpredictable environmental variations to the lines x environments interaction and verify if it is possible to reduce the number of evaluation environments of the Value for Cultivation and Use Trials (VCU) conducted in Minas Gerais, Brazil. We used grain yield data from 166 VCU trials of common bean conducted in the state from 2002 to 2012. Individual and joint analyses of variance of the environments were carried out for each two-year period and the contribution of each source of variation to total variation was estimated. Subsequently, ecovalence was used, and joint analyses of variance were made considering different numbers of environments by means of resampling. The source of variation that most contributes to the interaction is location. Reduction in the number of environments in the VCU trials is not a good strategy for recommendation of cultivars in Minas Gerais

Isolation of Infective Zika Virus from urine and saliva of patients in Brazil

Submitted by Sandra Infurna ([email protected]) on 2017-04-07T13:08:57Z No. of bitstreams: 1 mirna2_bonaldo_etal_IOC_2016.pdf: 2722947 bytes, checksum: 03f3dfbc2216beffa94b5996752a540d (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-04-07T13:26:32Z (GMT) No. of bitstreams: 1 mirna2_bonaldo_etal_IOC_2016.pdf: 2722947 bytes, checksum: 03f3dfbc2216beffa94b5996752a540d (MD5)Made available in DSpace on 2017-04-07T13:26:32Z (GMT). No. of bitstreams: 1 mirna2_bonaldo_etal_IOC_2016.pdf: 2722947 bytes, checksum: 03f3dfbc2216beffa94b5996752a540d (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica, Petrópolis, RJ, Brasil.Laboratório Nacional de Computação Científica, Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica, Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas.Rio de Janeiro, RJ, Brasil.Zika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission efficiency of its main putative vector, Ae. aegypti, in conjunction with apparent sexual transmission notifications, prompted the investigation of other potential sources of viral dissemination. Urine and saliva have been previously established as useful tools in ZIKV diagnosis. Here, we described the presence and isolation of infectious ZIKV particles from saliva and urine of acute phase patients in the Rio de Janeiro state, Brazil

ZIKV RNA detection and quantitation.

<p>ZIKV RNA detection and quantitation.</p

Isolation of Zika virus in Vero cell from the saliva of patient 6.

<p>Phase contrast optical microscopy of culture flasks containing (A) Mock-infected Vero cells and (B) saliva-infected Vero cells presenting a clear visible cytopathic effect. Viral plaque detection in saliva (C) and urine (D). The white arrow shows the unique viral plaque detected in the urine sample.</p

Differences in amino acid residues in ZIKV polyproteins of Rio-S1 and Rio-U1 isolates.

<p>Differences in amino acid residues in ZIKV polyproteins of Rio-S1 and Rio-U1 isolates.</p