Experimental investigation of the tire wear process using camera-assisted observation assessed by numerical modeling

This paper presents a novel experimental method to study the abrasion mechanism of car tires. It is based on the detection of microscopic movements associated with material damage (cracking) on the rubber tread. This is referred to as degrading layer relaxation. It correlates with the wear rate and, interestingly, the direction of the pattern's movement is opposite to the lateral forces during cornering. To measure and analyze the microscopic movements, a new camera-based method with feature point matching using video stabilization was developed. Besides extensive experimental investigation, the formation and propagation of microcracks are investigated using a simplified numerical model in which a phase field approach coupled with a viscoelastic constitutive behavior is implemented in a finite element framework

Resurrection Of Glyphohesione Friedrich, 1950, With Redescription Of G-Klatti Friedrich, 1950 And Description Of G-Longocirrata (Polychaeta, Hesionidae)

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Bidirectional cooperative motion of molecular motors

Recently, in a beautiful set of experiments, it has been shown that a Ncd mutant, NK11, which lacks directionality in its individual motion, was able to exhibit a new kind of directed motion in motility assays (Endow, S. A. & Higuchi, H. (2000) Nature (London) 406, 913–916): the filaments keep a given velocity for a while and then suddenly move in the opposite direction with similar velocity. We show that these observations nicely illustrate the concept of dynamic transitions in motor collections introduced earlier in the case of an infinite number of motors. We investigate the experimentally relevant case of a finite number of motors both when directionality is present (kinesins, myosins, Ncd) and absent (NK11). Using a symmetric two-state model, we demonstrate that bidirectional motion is the signature of a dynamic transition that results from the collective behavior of many motors acting on the same filament. For motors exhibiting directional bias individually, an asymmetric two-state model is appropriate. In that case, dynamic transitions exist for motor collections in the presence of an external force. We give predictions for the dependence of motion on ATP concentration, external forces, and the number of motors involved. In particular, we show that the reversal time grows exponentially with the number of motors per filament

Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors

The stepping behavior of single kinesin-1 motor proteins has been studied in great detail. However, in cells, these motors often do not work alone but rather function in small groups when they transport cellular cargo. Until now, the cooperative interactions between motors in such groups were poorly understood. A fundamental question is whether two or more motors that move the same cargo step in synchrony, producing the same step size as a single motor, or whether the step size of the cargo movement varies. To answer this question, we performed in vitro gliding motility assays, where microtubules coated with quantum dots were driven over a glass surface by a known number of kinesin-1 motors. The motion of individual microtubules was then tracked with nanometer precision. In the case of transport by two kinesin-1 motors, we found successive 4-nm steps, corresponding to half the step size of a single motor. Dwell-time analysis did not reveal any coordination, in the sense of alternate stepping, between the motors. When three motors interacted in collective transport, we identified distinct forward and backward jumps on the order of 10 nm. The existence of the fractional steps as well as the distinct jumps illustrate a lack of synchronization and has implications for the analysis of motor-driven organelle movement investigated in vivo