Research on the ZigBee Network and Equipment Design Based on the CC2530

It is known to us all that embedded systems designed on the 51 chip microcomputer are mature. This research finished to plant the system from 51 chip microcomputer to the CC2530 chip of TI. After analyzing and mastering the ZigBee 2007 PRO protocol stack, a new set of wireless sensor network (WSN) products has been developed. In the system, the coordinator can establish the Mesh network on its own initiative and the child nodes can join the network. The coordinator can be connected to the personal computer or the embedded gateway. The gateway had the Ethernet port and can send the data from the sensors to the cable or wireless network. The operator can issue instructions automatically and manually. The information of the sensor nodes can be returned and displayed on the serial debugging assistant. The equipment has passed the test with different environments in which there are obstacles among the communicating sensors and can be applied in the traffic, environmental monitoring and other fields widely

Data_Sheet_1_Self-esteem mediated relations between loneliness and social anxiety in Chinese adolescents with left-behind experience.CSV

Although research examining loneliness and social anxiety has been conducted, few studies have explored pathways from loneliness at home to social anxiety at school in Chinese left-behind children. The study attempts to explore associations between loneliness at home and social anxiety at school and to examine roles of self-esteem in those relationships among a sample of Chinese left-behind children. Date were collected from 303 left-behind children, aged 10–14 years, and the Chinese versions of Children’s Loneliness Scale, Social Anxiety Scale, and Rosenberg Self-esteem Scale were used to measure loneliness at home, social anxiety at school, and self-esteem, respectively. Results showed that loneliness at home was positively associated with social anxiety at school; self-esteem played a partial mediation role in associations between loneliness at home and social anxiety at school. Findings suggest that high levels of self-esteem may influence pathways from loneliness at home to social anxiety at school in Chinese left-behind children, and increasing levels of self-esteem may be used in preventions for loneliness of Chinese left-behind children.</p

Flavor explore: rapid prototyping and evaluation of user interfaces

Expert-evaluation methods, such as cognitive walkthrough and heuristic evaluation, are widely used in user experience studies due to the reason that it can identify usability problems early and fast and find areas for improvements. In this paper, we present evaluation of Flavor Explore, a high-fidelity prototype, aiming at searching for delicious food and nearby restaurants. A task-based evaluation was conducted to evaluate primary search tasks of the prototype, which included five experts with the background in interaction design and two potential users. Twenty two usability problems were then identified. The findings were helpful for improving the design and for further research

Toxic effects of combined exposure to homoyessotoxin and nitrite on the survival, antioxidative responses, and apoptosis of the abalone Haliotis discus hannai

Homoyessotoxin (homo-YTX) and nitrite (NO2-N), released during harmful dinoflagellate cell lysis adversely affect abalones. However, their toxicity mechanisms in shellfish remain unclear. This study investigated the economic abalone species Haliotis discus hannai exposed to varying concentrations of homo-YTX (0, 2, 5, and 10 µg L−1) and NO2-N (0, 3, and 6 mg L−1) on the basis of their 12 h LC50 values (5.05 µg L−1 and 4.25 mg L−1, respectively) and the environmentally relevant dissolved concentrations during severe dinoflagellate blooms, including mixtures. The test abalones were exposed to homo-YTX and NO2-N for 12 h. The mortality rate (D), reactive oxygen species (ROS) levels, antioxidant defense capabilities, and expression levels of antioxidant-related, Hsp-related, and apoptosis-related genes in abalone gills were assessed. Results showed that the combined exposure to homo-YTX and NO2-N increased the D and ROS levels and upregulated B-cell lymphoma-2 (BCL2)-associated X (BAX) and caspase3 (CASP3) expression levels while reducing glutathione peroxidase (GPx) activity and GPx, CuZnSOD, and BCL2 expression levels. High concentrations of homo-YTX (10 µg L−1) and NO2-N (6 mg L−1) solutions and the combinations of these toxicants inhibited the activities of superoxide dismutase (SOD) and catalase (CAT) and downregulated the expression levels of MnSOD, CAT, Hsp70, and Hsp90. The ROS levels were negatively correlated with the activities of SOD, CAT, and GPx and the expression levels of MnSOD, CuZnSOD, CAT, GPx, Hsp70, Hsp90, and BCL2. These results suggest that homo-YTX, in conjunction with NO2-N, induces oxidative stress, disrupts antioxidant defense systems, and triggers caspase-dependent apoptosis in the gills of abalone. ROS-mediated antioxidative and heat-shock responses and apoptosis emerge as potential toxicity mechanisms affecting the survival of H. discus hannai due to homo-YTX and NO2-N exposure

Osteopontin, Bone Morphogenetic Protein-4, and Vitamin D Receptor Gene Polymorphisms in the Susceptibility and Clinical Severity of Spinal Tuberculosis

Background/Aims: Spinal tuberculosis (TB) is a common and dangerous form of extrapulmonary TB with unclear mechanisms in its occurrence and progression. This study investigated the clinical significances of bone morphogenetic protein-4 (BMP-4), osteopontin (OPN), and vitamin D receptor (VDR) gene polymorphism, mRNA and protein expression in spinal TB patients. Methods: BMP-4 and OPN gene polymorphisms were detected by direct DNA sequencing, while VDR-FokI polymorphisms were analyzed using PCR-RFLP. mRNA and protein expression was measured using real-time PCR and Western blot, respectively. Results: A significant lower frequency of TT genotype and T allele at 6007C&#x3e;T polymorphism in BMP-4 gene; higher frequency of GG genotype and G allele at -66T&#x3e;G polymorphism in OPN gene, and higher frequency of the ff genotype and f allele at the VDR-FokI polymorphism were observed in patients with spinal TB compared to controls. TT genotype of 6007C&#x3e;T polymorphism correlated with a lower BMP-4 mRNA and protein expression, -66GG genotype correlated with a high OPN mRNA and protein expression, and ff genotype correlated with the lower VDR mRNA and protein levels in the intervertebral disc tissues. The TT genotype and low BMP-4 gene expression; the -66GG genotype and high OPN gene expression; and the ff genotype and low VDR gene expression significantly correlated with the clinical severity of spinal TB. Conclusion: The 6007C&#x3e;T polymorphism of BMP-4, -66T&#x3e;G polymorphism of OPN, and VDR-FokI polymorphism are the susceptible factors of spinal TB and indicators of the clinical severity. These three genes may collaborate in the development of spinal TB

Mesenchymal Migration on Adhesive–Nonadhesive Alternate Surfaces in Macrophages

Abstract Mesenchymal migration usually happens on adhesive substrates, while cells adopt amoeboid migration on low/nonadhesive surfaces. Protein‐repelling reagents, e.g., poly(ethylene) glycol (PEG), are routinely employed to resist cell adhering and migrating. Contrary to these perceptions, this work discovers a unique locomotion of macrophages on adhesive–nonadhesive alternate substrates in vitro that they can overcome nonadhesive PEG gaps to reach adhesive regions in the mesenchymal mode. Adhering to extracellular matrix regions is a prerequisite for macrophages to perform further locomotion on the PEG regions. Podosomes are found highly enriched on the PEG region in macrophages and support their migration across the nonadhesive regions. Increasing podosome density through myosin IIA inhibition facilitates cell motility on adhesive–nonadhesive alternate substrates. Moreover, a developed cellular Potts model reproduces this mesenchymal migration. These findings together uncover a new migratory behavior on adhesive–nonadhesive alternate substrates in macrophages

G-protein-coupled receptor kinase 4 causes renal angiotensin II type 2 receptor dysfunction by increasing its phosphorylation

Activation of the angiotensin II type 2 receptor (AT2R) induces diuresis and natriuresis. Increased expression or/and activity of G-protein-coupled receptor kinase 4 (GRK4) or genetic variants (e.g., GRK4γ142V) cause sodium retention and hypertension. Whether GRK4 plays a role in the regulation of AT2R in the kidney remains unknown. In the present study, we found that spontaneously hypertensive rats (SHRs) had increased AT2R phosphorylation and impaired AT2R-mediated diuretic and natriuretic effects, as compared with normotensive Wistar-Kyoto (WKY) rats. The regulation by GRK4 of renal AT2R phosphorylation and function was studied in human (h) GRK4γ transgenic mice. hGRK4γ142V transgenic mice had increased renal AT2R phosphorylation and impaired AT2R-mediated natriuresis, relative to hGRK4γ wild-type (WT) littermates. These were confirmed in vitro; AT2R phosphorylation was increased and AT2R-mediated inhibition of Na+-K+-ATPase activity was decreased in hGRK4γ142V, relative to hGRK4γ WT-transfected renal proximal tubule (RPT) cells. There was a direct physical interaction between renal GRK4 and AT2R that was increased in SHRs, relative to WKY rats. Ultrasound-targeted microbubble destruction of renal GRK4 decreased the renal AT2R phosphorylation and restored the impaired AT2R-mediated diuresis and natriuresis in SHRs. In vitro studies showed that GRK4 siRNA reduced AT2R phosphorylation and reversed the impaired AT2R-mediated inhibition of Na+-K+-ATPase activity in SHR RPT cells. Our present study shows that GRK4, at least in part, impairs renal AT2R-mediated diuresis and natriuresis by increasing its phosphorylation; inhibition of GRK4 expression and/or activity may be a potential strategy to improve the renal function of AT2R