Amplifying Frequency Up-Converted Infrared Signals with a Molecular Optomechanical Cavity

Frequency up-conversion, enabled by molecular optomechanical coupling, has recently emerged as a promising approach for converting infrared signals into the visible range through quantum coherent conversion of signals. However, detecting these converted signals poses a significant challenge due to their inherently weak signal intensity. In this work, we propose an amplification mechanism capable of enhancing the signal intensity by a factor of 1000 or more in a molecular-cavity system consisting $10^{7}$ molecules. The mechanism takes advantage of the strong coupling enhancement with molecular collective mode and Stokes sideband pump. Our work demonstrates a feasible approach for up-converting infrared signals to the visible range.Comment: 5 pages, 4 figures for the main text; 6 pages, 2 figures for Supplementary Materia

BCL9 enhances the development of cervical carcinoma by deactivating CPEB3/EGFR axis

Purpose: To investigate the differential expression of BCL9 in cervical carcinoma samples, analyze its biological functions in regulating malignant phenotypes of cervical carcinoma cells, and to explore its potential molecular mechanism.Methods: Expression levels of BCL9 in 58 pairs of cervical carcinoma tissues and paracancerous tissues were determined using quantitative real time-polymerase chain reaction (qRT-PCR). Kaplan- Meier curves were used to analyze the prognostic potential of BCL9 in cervical carcinoma. After knockdown using BCL9 by lentivirus transfection, proliferative and migratory changes in Siha and HeLa cells were determined by CCK-8, colony formation and Transwell assays. Cytoplasmic polyadenylation element binding protein 3 (CPEB3), the potential downstream target of BCL9, was confirmed via dualluciferase reporter assay. Western blot analyses were conducted to determine the protein levels of CPEB3, EGFR, AKT and p21 in Siha and HeLa cells with BCL9 knockdown. The co-regulation of BCL9 and CPEB3 on phenotypes of cervical carcinoma cell was investigated.Results: BCL9 was upregulated in cervical carcinoma tissues. The high level of BCL9 was predicted by the tumor size, advanced stage and poor prognosis. The knockdown of BCL9 significantly weakened proliferative and migratory abilities of Siha and HeLa cells (p < 0.05). CPEB3 was the downstream target of BCL9, and was lowly expressed in cervical carcinoma tissues. The knockdown of BCL9 upregulated CPEB3, and downregulated EGFR, AKT and p21 (p < 0.05). The knockdown of CPEB3 also reversed the influence of silenced BCL9 in regulating its proliferative and migratory abilities in cervical carcinoma cells (p < 0.05).Conclusion: BCL9 drives the deterioration of cervical carcinoma by inhibiting the CPEB3/EGFR axis.Thus, BCL9 may be a novel molecular target for cervical carcinoma treatment

The Roles of PI3K/AKT/mTOR and MAPK/ERK Signaling Pathways in Human Pheochromocytomas

Objectives. The roles of PI3K/AKT/mTOR and MAPK/ERK pathways involved in the pathogenesis of pheochromocytoma and paraganglioma (PPGL) were demonstrated mostly by in vitro studies with rat or mouse cells and were mainly studied at transcriptional level. This study aimed to investigate the effect of these pathways on the proliferation of human PPGL cells and the activation of these pathways in PPGLs. Methods. Human PPGL cells were treated with sunitinib and inhibitors of PI3K (LY294002), MEK1/2 (U0126), and mTORC1/2 (AZD8055). Cell proliferation was detected by MTT assay. Protein phosphorylation was detected by Western blotting. Results. In most PPGLs, AKT, ERK1/2, and mTOR were activated. LY294002 (10 μM), U0126 (10 μM), AZD8055 (1 μM), and sunitinib (1 μM) inhibited PPGL cell proliferation in ten primary cultures of tissues, including four from patients with gene mutations. MEK1/2 inhibitor decreased mTOR phosphorylation. Inhibition of mTOR reduced phosphorylation of AKT and ERK1/2. Sunitinib inhibited phospho-ERK1/2 and phospho-mTOR. Conclusion. Our study suggested that PI3K/AKT/mTOR and MAPK/ERK signaling pathways play vital roles in human PPGL and are activated in most PPGLs. Inhibiting multiple pathways might be a novel therapeutic approach for PPGLs

Design of double band Beidou navigation antenna with wide axial ratio beam

ObjectivesIn order to effectively broaden the beam width of the 3 dB axis ratio of a Beidou antenna, this paper proposes a slotted laminated microstrip patch antenna loaded with parasitic elements. MethodsThe Beidou dual frequency band feature is realized through a two-layer substrate structure. The bandwidth is widened by slotting. Circular polarized radiation is achieved by cutting corners at the edge of the patch. At the same time, L-shaped parasitic elements are loaded around the patch to broaden the 3 dB axial ratio beam width of the antenna.ResultsThe measured and simulated results are in good agreement. The relative bandwidths of the antenna in the Beidou B1 and B2 bands are 3% and 7.5%. The beam width of the 3 dB axis ratio can reach 180° and 176°, and the maximum gain can reach 6 dBi. In the Beidou receiver test, the number of antenna satellites can reach more than 17, and the positioning accuracy is within meters. ConclusionsThis paper proposes and validates a high-precision positioning dual band Beidou navigation antenna with wide axis ratio beam width. The results can provide references for the design of small Beidou navigation antennas

Immune-related ureteritis and cystitis induced by immune checkpoint inhibitors: Case report and literature review

Immune checkpoint inhibitors (ICIs), including anti-cytotoxic T lymphocyte-associated protein 4 (anti-CTLA4) and anti-programmed death cell protein 1 (anti-PD-1), are increasingly prescribed in metastatic carcinoma therapy. ICI-related kidney injury is gradually recognized by clinicians. However, immune-related ureteritis and cystitis easily go undiagnosed. We report three cases of PD-1 monoclonal antibody (mAb)-related ureteritis and cystitis. We further carried out a review of the literature about ICI-related ureteritis and cystitis. The cases in our reports manifest urinary irritation, sterile pyuria, gross hematuria, hydronephrosis, dilation of the ureters, and acute kidney injury. Urinary irritation improved effectively; urinalysis and renal function returned to normal after glucocorticoid therapy. During ICI therapy, urinalysis and renal function and urinary imaging examination are recommended to be monitored regularly. It contributes to identify immune-related ureteritis/cystitis earlier to efficiently alleviate urinary symptoms and immunologic urinary tract injury through glucocorticoid therapy while avoiding the abuse of antibiotics

Stellar Atmospheric Parameters for Cool Dwarfs in Gaia DR3

We provide a catalogue of atmospheric parameters for 1,806,921 cool dwarfs from Gaia DR3 which lie within the range covered by LAMOST cool dwarf spectroscopic parameters: 3200 K < T_{eff}< 4300 K, -0.8 < [M/H] < 0.2 dex, and 4.5 <log{g} < 5.5 dex. Our values are derived based on Machine Learning models trained with multi-band photometry corrected for dust. The photometric data comprises of optical from SDSS r, i, z bands, near-infrared from 2MASS J, H, K and mid-infrared from ALLWISE W1, W2. We used both random forest and LightGBM machine learning models and found similar results from both with an error dispersion of 68 K, 0.22 dex, and 0.05 dex for T_{eff}, [M/H], and log {g}, respectively. Assessment of the relative feature importance of different photometric colors indicated W1 -- W2 as most sensitive to both T_{eff} and log{g}, with J -- H most sensitive to [M/H]. We find that our values show a good agreement with APOGEE, but are significantly different to those provided as part of Gaia DR3.Comment: 14 pages, 12 figures, accepted by ApJ

Genetic Polymorphisms In Estrogen-related Genes And The Risk Of Breast Cancer Among Han Chinese Women

Exposure to high levels of estrogen is considered an important risk factor for susceptibility to breast cancer. Common polymorphisms in genes that affect estrogen levels may be associated with breast cancer risk, but no comprehensive study has been performed among Han Chinese women. In the present study, 32 single-nucleotide polymorphisms (SNPs) in estrogen-related genes were genotyped using the MassARRAY IPLEX platform in 1076 Han Chinese women. Genotypic and allelic frequencies were compared between case and control groups. Unconditional logistic regression was used to assess the effects of SNPs on breast cancer risk. Associations were also evaluated for breast cancer subtypes stratified by estrogen receptor (ER) and progesterone receptor (PR) status. Case-control analysis showed a significant relation between heterozygous genotypes of rs700519 and rs2069522 and breast cancer risk (OR = 0.723, 95% CI = 0.541-0.965, p = 0.028 and OR = 1.500, 95% CI = 1.078-2.087, p = 0.016, respectively). Subgroup comparisons revealed that rs2446405 and rs17268974 were related to ER status, and rs130021 was associated with PR status. Our findings suggest that rs700519 and rs2069522 are associated with susceptibility to breast cancer among the Han Chinese population and have a cumulative effect with three other identified SNPs. Further genetic and functional studies are needed to identify additional SNPs, and to elucidate the underlying molecular mechanisms

Stellar Atmospheric Parameters for Cool Dwarfs in Gaia Data Release 3

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/We provide a catalog of atmospheric parameters for 1,806,921 cool dwarfs from Gaia Data Release 3 (DR3) that lie within the range covered by LAMOST cool dwarf spectroscopic parameters: 3200 K < T eff < 4300 K, −0.8 < [M/H] < 0.2 dex, and 4.5 < log g < 5.5 dex. Our values are derived based on machine-learning models trained with multiband photometry corrected for dust. The photometric data comprise optical data from the Sloan Digital Sky Survey r, i, and z bands, near-infrared data from the Two Micron All Sky Survey J, H, and K bands, and mid-infrared data from the ALLWISE W1 and W2 bands. We used both random forest and light gradient boosting machine machine-learning models and found similar results from both, with an error dispersion of 68 K, 0.22 dex, and 0.05 dex for T eff, [M/H], and log g, respectively. Assessment of the relative feature importance of different photometric colors indicated W1 − W2 as most sensitive to both T eff and log g, with J − H being most sensitive to [M/H]. We find that our values show a good agreement with the Apache Point Observatory Galactic Evolution Experiment, but are significantly different to those provided as part of Gaia DR3.Peer reviewe

Alternative O-GlcNAcylation/O-Phosphorylation of Ser16 Induce Different Conformational Disturbances to the N Terminus of Murine Estrogen Receptor β

SummarySerine and threonine residues in many proteins can be modified by either phosphorylation or GlcNAcylation. To investigate the mechanism of O-GlcNAc and O-phosphate's reciprocal roles in modulating the degradation and activity of murine estrogen receptor β (mER-β), the conformational changes induced by O-GlcNAcylation and O-phosphorylation of Ser16 in 17-mer model peptides corresponding to the N-terminal intrinsically disordered (ID) region of mER-β were studied by NMR techniques, circular dichroism (CD), and molecular dynamics simulations. Our results suggest that O-phosphorylation discourages the turn formation in the S15STG18 fragment. In contrast, O-GlcNAcylation promotes turn formation in this region. Thus, we postulate that the different changes of the local structure in the N-terminal S15STG18 fragment of mER-β caused by O-phosphate or O-GlcNAc modification might lead to the disturbances to the dynamic ensembles of the ID region of mER-β, which is related to its modulatory activity